Journal of Veterinary Science

The Korean Society of Veterinary Science (대한수의학회)
권호 표지

Protective Effects of N-acetylcysteine and Selenium against Doxorubicin Toxicity in Rats

Park, Eun-Sung;Kim, Sun-Don;Lee, Min-Hye;Lee, Heung-Shik S.;Lee, In-Se;Sung, Je-Kyung;Yoon, Yeo-Sung

  • Published : 20030000
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Abstract

To investigate the neutralizing effect of N-acetylcysteine (NAC) and selenium (Se) aganist doxorubicin (DOX) toxicity in rats, NAC (140 mg/kg, p.o.) and Se(0.5 mg/kg, p.o.) were administered for 2 days before DOX injection and then 3 times a week. Cell viability and the level of lipid peroxidation were examined in cultured-rat astrocytes, Severe morphologic changes in the kidney of DOX group; thickening of Bowmans capsule, presence of multifocal tubular casts were observed, but not in the other treated groups. Vacuoles in some hepatic cells and focal aggregation of stellate macrophages were also detected in DOX group, but not in the other treated groups. However, the severe inhibition of spermatogenesis was found in all treated groups. The cell viability of DOX (10 mg/ml) treated group and NAC (5 mM) or Se (0.001 mg/ml) combined-treated group was 52.5${\pm}$2.0 %, 85.3${\pm}$4.5 % and 75.5${\pm}$1.6 % respectively. In MDA (malondialdehyde) assay, the level of lipid peroxidation on DOX (10 mg/ml), NAC (5 mM) and Se (0.001 mg/ml) was 0.77${\pm}$0.06, 0.35${\pm}$0.06 and 0.54${\pm}$

Keywords

References

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