Horticultural Science & Technology (원예과학기술지)

Korean Society of Horticultural Science (한국원예학회)
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Pollen Germination of Hemerocallis spp. Affected by Media Type andStorage Temperature

배지종류와 저장온도에 따른 원추리속(Hemerocallis spp.)의 화분발아

An, Min-Sil;Jo, Jong-Hyeon;Choe, So-Ra;Im, Hoe-Chun;Choe, Dong-Chil;Park, Yun-Jeom
안민실;조종현;최소라;임회춘;최동칠;박윤점

  • Published : 20030000
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Abstract

The effects of media type, storage temperature on pollen germination of Hemerocallis spp. were examined. The percentage of pollen germination was higher in all of media type than in the basal medium (sucrose 15% + agar 5%). Especially, GN and GL media including such as CaCl_2, KNO_3 and Ca(NO_3)․H_2O were more effective to improve the pollen germination than the GC medium including only H_3BO_3. Irrespective of species, periodical germination percentage according to storage temperature showed a strong decline from 2days in room temperature storage. In the case of 4$\circ$C, H. dumortieri and H. thunbergii showed a rapid decline pollen viability from 30days after storage, but the pollen in -20$\circ$C storage was maintained pollen viability above 40% until 180 days after storage. Since -20$\circ$C storage for pollen viability is effective, interspecific and intergeneric cross could be possible despite differnet flowering period.

배지종류와 저장온도가 원추리속의 화분발아에 미치는 영향을 조사하였다. 무기물이 첨가된 배지 종류별 화분 발아율은 4종 모두 sucrose 15% + agar 5%보다 발아율이 높아졌으며, H3BO3 만 함유된 GC배지보다는 CaCl2, KNO3, Ca(NO3)·H2O가 첨가된 GN과 GL배지가 발아율 향상에 효과적이었다. 저장온도와 기간에 따른 발아율은 상온 저장에서는 종에 관계없이 모두 2일째부터 현격히 저하되었고, 4℃ 저장의 경우 각시원추리와 노랑원추리는 30일 후부터 급격히 화분 활력이 저하되었으나, -20℃ 저장에서는 180일 후에도 40% 이상의 화분 활력을 지니고 있었다. 그러므로 화분 활력 유지에 효과적인 -20℃ 저장으로 개화기가 상이한 종· 속간 상호 교배가 가능하리라 생각된다.

Keywords

References

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