Horticultural Science & Technology (원예과학기술지)

Korean Society of Horticultural Science (한국원예학회)
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Virus-Infected Status in Imported Bulbs of Lilium Oriental Hybrids

오리엔탈 나리 수입 구근의 바이러스 감염 정도

Park, Kyeung-Il;Choi, Jeong-Doo;Park, In-Sook;Eum, Sun-Jung;Kim, Kiu-Weon
박경일;최정두;박인숙;엄선정;김규원

  • Published : 20030000
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Abstract

The study was performed to investigate the virus infection status of imported Lilium Oriental Hybrids bulbs. Many virus particles were observed through direct negative staining methods (DN) in virus-infected leaves of the lily ‘Casa Blanca’. Among those, filamentous virus of about 650 nm in length was identified as lily symptomless virus (LSV), and virus of about 750 nm was presumed to be as TBV lily stain (TBV-L). This may not be a tulip breaking virus (TBV), as examined by an immunosorbent electron microscope (ISEM). An unknown virus in Lilium was observed, and the virus was enormous filamentous particles of about 2,000 nm in length. In detecting the virus by enzyme-linked immunosorbent assay (ELISA) and direct tissue blotting immunoassay (DTBIA), it was confirmed that ‘Casa Blanca’ was infected with LSV in the range of 50.0󰠏77.5%, cucumber mosaic virus (CMV) 10.0󰠏37.5% and TBV 75.0󰠏97.5%. Twenty percenty of ‘Acapulco’ was infected with LSV, 2.5󰠏7.5% with CMV and 15.0󰠏35.0% with TMV. In ‘Casa Blanca’ and ‘Acapulco’, the rates of plants infected with one virus were 7.5% and 25.0%, with two viruses were 70.0% and 22.5%, and with three viruses were 22.5% and 5%, respectively. Sugar contents in leaves and scales were higher in healthy plants than virus-infected plants.

수입 나리구근의 품질에 대한 이해를 바르게 하고 절화재배 및 구근생산에 필요한 기초자료를 얻고자 오리엔탈 나리의 수입 구근을 재료로 Enzyme-linked immuno-sorbent assay와 direct tissue blotting immunoassay에 의해 바이러스 감염 실태를 조사한 결과, 'Casa Blanca'는 100%가 바이러스에 감염되어 있었으며 LSV 50.0 - 77.5%, CMV 10.0 - 37.5%, TBV 75.0 - 97.5%의 범위였다. 'Acapulco'는 LSV 20.0%, CMV 2.5 - 7.5%, TBV 15.0 - 35.0%의 범위로 감염되어 있었다. 또한 한 종에 의한 단독 감염주는 'Casa Blanca' 7.5%, 'Acapulco' 25.0%이었고, 두 종의 바이러스에 의한 복합 감염주는 'Casa Blanca' 70.0%, 'Acapulco' 20.0%, 그리고 세 종의 바이러스에 의한 복합 감염주는 'Casa Blanca' 22.5%, 'Acapulco' 5%이었다. 'Casa Blanca'의 이병주 잎으로부터 direct negative staining에 의해 다량의 사상형 바이러스 입자가 관찰되었다. 이들 가운데 650nm의 사상형 바이러스는 immunosorbent electron microscopy법에 의해 LSV로 동정되었으며, 750nm의 바이러스는 TBV-L일 것으로 추정되었다. 그리고 나리에서는 보고된 바 없는 약 2,000nm 크기의 긴 바이러스도 관찰되었다. 잎과 인편의 당 함량은 바이러스 이병주에 비해 무병주에서 높았다.

Keywords

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