Food Science and Biotechnology

Korean Society of Food Science and Technology (한국식품과학회)
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Isoflavone Content and Estrogen Activity in Arrowroot Puerariae Radix

Kim, Hee-Yun;Hong, Jin-Hwan;Kim, Dong-Sul;Kang, Kil-Jin;Han, Sang-Bae;Lee, Eun-Ju;Chung, Hyung-Wook;Song, Kyung-Hee;Sho, Kyung-A;Kwack, Seung-Jun;Kim, Soon-Sun;Park, Kui-Lea;Lee, Si-Kyung;Kim, Myung-Chul;Kim, Chang-Min;Song, In-Sang

  • Published : 20030200
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Abstract

The content of isoflavone and the estrogenic activity in Puerariae Radix were investigated. HPLC using a dual column system was used to determine the content of isoflavone in Puerariae Radix. The simultaneous analytical time of isoflavones (daidzin, genistin, daidzein, glycitein, genistein) required less than 25 min per sample. The content of daidzin $(5,026-18,547\;{\mu}g/g) $ genistin $(488-1,201\;{\mu}g/g) $, daidzein $(316-876\;{\mu}g/g) $, genistein $(8-63\;{\mu}g/g) $, puerarin (1-10 g/l00 g) in Pueraria thunbergiana showed higher levels compared to content of daidzin $(168\;{\mu}g/g) $ genistin $(62\;{\mu}g/g) $, daidzein $(46\;{\mu}g/g) $, puerarin (0.03 g/100 g) in Pueraria mirifica. Glycitein was not detected in Puerariae Radix. In uterotrophic assay as indicator of estrogenic activity, the Pueraria mirifica exhibited strong estrogenic activity, whereas Pueraria thunbergiana and Butea superba did not. Therefore, the content of isoflavones in Puerariae Radix could not be responsible for estrogenic activity.

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References

  1. Doosan World Encyclopedia. 25
  2. Pharmacopoeia of People's Republic of China, 1 The Public Health Department of People's Republic of China
  3. An Estrogen from the plant Pueraria mirifica James, C.C.
  4. Journal of Natural Products v.63 no.2 Identification of deoxymiroestrol as the actual rejuvenating principle of 'Kwao Keur', Pueraria mirifica. The known miroestrol may be an artifact Sunee, C.;Tsutomu, I.;Hiroko, S.;Keiko, S.;Mineaki, O.;Chaiyo, C. https://doi.org/10.1021/np990547v
  5. Planta Medica v.66 no.6 Isoflavonoides from Pueraria mirifica and their estrogenic activity Chansakaow, S.;Ishikawa, T.;Sekine, K,;Okada, M.;Higuchi, Y.;Kudo, M.;Chaichantipyuth, C. https://doi.org/10.1055/s-2000-8603
  6. Lancet v.350 Exposure of infants to phyto-estrogens from soy-based infant formula Kenneth, D.R.;Setchell, L.Z.N.;Jinnan, C.;James, E.
  7. Korean J. Nutr. v.34 Response relationship of isoflavone supplementation on plasma lipid profiles and total antioxidant status in peri menopausal and postmenopimsal women Choi, Y.;Yoon, S.;Lee, M.J.;Lee, S.K.;Lee, B.S.
  8. Journal of the American Dietetic Association v.95 no.5 Urinary isoflavonoid phyroestrogen and lignan extraction after consumption of fermented and unfermented soy products Andrea, M.;Hutchins, R.D.;Joanne, L.;Slavin, R.D. https://doi.org/10.1016/S0002-8223(95)00149-2
  9. Journal of Pharmaceutical and Biomedical Analysis v.14 no.1 An investigation on the extraction and concentration of isoflavones in soy-based products Thuylinh, N.;Euphemia, W.;Andrew, P.C. https://doi.org/10.1016/0731-7085(95)01619-8
  10. Journal of Chromatography A v.923 no.1-2 Dertermination of puerarin, daidzeiri and rutin in Pueraria lobata (Wild.) Ohwi by capillary electrophoresis with electrochemical detection Gang, C.;Jianxia, Z.;Jiannong, Y. https://doi.org/10.1016/S0021-9673(01)00996-7
  11. The Korea Pharmacopoeia
  12. Yakugaku Zasshi v.104 Studies on the physical and chemical quality evaluation of crude drugs preparations, I. Analysis of pueraria radix and spices puerariae Hayakawa, J.;Noda, S.Y.;Uno, K.
  13. Korean J. Food Hyg. v.2 Studies on the content of Pueraria Radix in the tea by HPLC Kim, M.H.;Park, S.B.
  14. Korean J. Food Sci. Technol. v.22 Antioxidative components of Pueraria root Oh, M.J.;Lee, K.S.;Son, H.Y.;Kim, S.Y.
  15. Fundam. Appl. Toxicol. Pharmacol. v.25 Survey and assessment of mammalian estrogen biological assays for hazard characterization Reel, J.R.;Labb, L.V.J.;Neal, B.H.
  16. Fundam. Appl. Toxicol. Pharmacol. v.25 Paradoxical effects of the soy phytoestrogen, genistein on growth of human breast cancer cells in vitro and in vivo Helferich, W.G.
  17. Nat. Inst. Toxicol. Res. v.2 Effects of alkylphenolic compounds on estrogenic activity in vitro and in vivo Kwack, S.J.;Kwon, O.;Kim, S.S.;Kim, H.S.;Shin, J.H.;Kim, S.H.;Sohn, K.H.;Moon, K.H.;Chae, S.Y.;Lee, R.D.;Lee, H.S.;Park, C.H.;Jeung, E.B.;Han, S.Y.;Kil, K.S.;Park, K.L.
  18. Nat. Inst. Toxicol. Res. v.2 International cooperation study for evaluation on OECD screening and testing method for endocrine disruptors Han, S.Y.;Kim, H.S.;Shin, J.H.;Moon, H.J.;Kim, I.S.;Kang, I.H.;Kim, L.Y.;Suk, J.H.;Whang, I.K.;Oh, S.D.;Kim, S.H.;Lee, R.D.;Park, K.L.;Ha, K.W.
  19. Endocrine disruptors scerening and testing, Agenda Item 5, Validation protocol for the uterotrophic assay OECD;Validation Management Committe
  20. Endocrine Disruptor Screening and Testing Advisory Committee Final Report EPA
  21. Journal of Agricultural and Food Chemistry v.42 no.9 Quantitation of phytoestrogens in legumes by HPLC Franke, A.A.;Custer, L.J.;Cerna, C.M.;Narala, K.K. https://doi.org/10.1021/jf00045a015
  22. Korean J. Food Sci. Technol. v.32 High Performance liquid chromatographic analysis of isoflavones in soybean foods Kim, C.S.;Lee, Y.S.;Kim, J.S.;Hahn, Y.H.