Journal of The Korean Ophthalmological Society (대한안과학회지)

The Korean Ophthalmological Society (대한안과학회)
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Effects of Taxol and Mitomycin C on Morphologic Alteration of Cultured Rabbit Subconjunctival Fibroblasts

배양된 가토의 결막하 섬유모세포에 Taxol과 Mitomycin C가 미치는 형태학적인 변화

Baek, Yong-Woo;Moon, Jung-Il;Park, Chan-Kee
백용우;문정일;박찬기

  • Published : 20030100
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Abstract

Purpose : Scar tissue formation by cell proliferation, migration and contraction of fibroblasts may result in the failure of trabeculectomy. The author investigated effects of Mitomycin C (MMC) and Taxol on morphologic alteration of cultured rabbit subconjunctival fibroblasts. Methods : Indirect immunofluorescence microscopy, with antibodies against tubulin, was used to examine the effect of Mitomycin C and Taxol on cytoplasmic microtubules in rabbit. Tenon's fibroblast cells and Acridine orange/Ethidium bromide dyes were used to determine cell viability and apoptosis at various concentrations of MMC and Taxol. Results : Taxol treated cell showed an increased intensity of microtubule staining around the nuclear region and morphologic differences in overall cell shape, and MMC treated cells were noted more prominent apoptotic changes. Conclusions : Our result suggested that Taxol might be as potent inhibitor of fibroblast proliferation as Mitomycin C, and therefore, might be used for improving the success rate of trabeculectomy.

목적 : 녹내장 여과 수술 후 섬유모세포의 증식, 이동, 수축에 의한 반흔 조직의 형성은 여과 수술의 실패를 초래할 수 있다. 연구자는 Mitomycin C (MMC)와 Taxol이 배양된 토끼의 테논낭 섬유모세포의 형태 변화에 미치는 영향을 알아보고자 하였다. 대상과 방법 : MMC와 Taxol이 섬유모세포 이동에 관여하는 미세소관(microtubule)에 미치는 영향을 알아보기 위해 튜불린에 대한 항체를 이용한 면역 형광 현미경 관찰을 하였다. 또한 세포의 생존과 고사(apoptosis)를 형태학적으로 관찰하기 위해 Acridine orange/Ethidium bromide 염색법을 사용하였다. 결과 : Taxol을 처리한 군에서는 대조군이나 MMC를 처리한 군에 비해 세포 내에 미세소관이 증가하였고 특히 핵 주위에서 두드러지게 강하게 염색되었으며 세포 모습의 변형이 일어났다. 그리고 MMC를 처리한 군에서 더 현저한 세포의 고사가 관찰되었다. 결론 : 이상의 결과로 Taxol은 MMC와 같은 강력한 섬유모세포 증식 억제 능력을 가지고 있음을 확인하였으며 향후 녹내장 여과 수술에서 또 하나의 효과적인 보조 약제로 사용될 수 있을 것으로 생각된다.

Keywords

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