Horticulture, Environment, and Biotechnology : HEB

Korean Society of Horticultural Science (한국원예학회)
권호 표지

Development of an Efficient Screening System for Anthracnose Resistance in Grapes

온실 내에서의 효율적인 포도 새눈무늬병 저항성 검정법 개발

Yun, Hae-Keun;Park, Kyo-Sun;Rho, Jeong-Ho;Kwon, Byung-Oh;Jeong, Sang-Bouk
윤해근;박교선;노정호;권병오;정상복

  • Published : 20030000
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Abstract

Anthracnose (Elsinoe ampelina) occurs severely in grapevines grown in warm and humid climate regions such as Korea. Hence, resistance of some cultivars to anthracnose is one of the big advantages in grapevine cultivation. Breeding of cultivars resistant to anthracnose is one of the most important grape breeding goals in Korea. The objective of this study is to develop a rapid screening system by using very young seedlings in selecting the resistant cultivars and progenies against anthracnose. Among three different concentrations of pathogens, spore suspension of 1105 spores/mL was optimum for symptom development by spray inoculation. After inoculation, the plants were incubated in a moisture chamber for 48 to 72 h, which later resulted in good symptom development. The most effective plant materials for screening were seedlings or cuttings in the five or seven true-leaf stage. Lesion scoring in the third or fourth leaves was efficient in distinguishing resistant cultivars from susceptible ones to anthracnose. Some cultivars such as Rizamat and Kaiji were susceptible, while Kyoho and Benifuji were moderately susceptible and Campbell Early and Niagara were found to be resistant to the pathogen inoculation of anthracnose in a greenhouse. This pattern was consistent with that from field screening. This greenhouse procedure for selecting grapevine cultivars with resistance to anthracnose was found to be accurate, economical, and labor-saving.

최근 국내에서 포도 재배 시 포도 새눈무늬병의 발생이 증가하면서 주요한 포도 병해 중의 하나로 부각되어 이에 대한 효율적인 포도 품종 간 저항성 검정 시스템을 개발하고자 하였다. 품종 저항성 검정 및 포도 신품종 육성을 위한 교배 실생의 유묘를 시험 재료로 이용하였다. 인위적인 병원균 포자 현탁액 분무 접종에는 105spores/mL의 병원균 농도가 효율적으로 병반 형성을 유도하였으며, 병원균 접종 후 48시간 또는 72시간의 포화 습도의 유지 기간이 품종 간 저항성 검정에 효율적이었다. 인공 접종에 의한 포도 새눈무늬병 저항성 검정에는 5-7개 내외의 본엽을 유지한 교배 실생 또는 삽목 묘가 효율적이며 3엽과 4엽이 병반 형성에 가장 뚜렷한 차이를 나타내었다. 병원균의 인공 접종 결과 ‘Rizamat’와 ‘Kaiji’는 감수성, ‘Kyoho’와 ‘Benifuji’는 중도 감수성, ‘Campbell Early’와 ‘Niagara’는 저항성 품종이었으며 이 결과는 포장 상태에서의 저항성 차이와 일치하는 경향을 나타내었다. 본 연구에서 개발한 온실 내에서의 병원균 인공 접종법을 이용하여 포도 새눈무늬병에 대한 품종 간 저항성 검정을 효율적으로 수행할 수 있었다.

Keywords

References

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