Annals of Surgical Treatment and Research

The Korean Surgical Society (대한외과학회)
권호 표지

Interactions of Nitric Oxide and Antioxidants in Hypoxia-Reoxygenation Injury

저산소-재산소화 손상에 미치는 Nitric Oxide에 대한 항산화제의 상호작용

Son, Young-Gil;Kim, Kwang-Il;Jo, Hae-Chang;Rhee, Jung-Ahn;Bae, Byung-Jo;Choi, Hyung-Chul;Lee, Kwang-Yoon;Kim, Won-Jun
손영길;김광일;조해창;이정안;배병조;최형철;이광윤;김원준

  • Published : 20030400
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Abstract

Purpose: An ischemia-reperfusion injury leads to profound functional and structural alterations of vascular smooth muscle cells (VSMC). It is still not clear whether hypoxiareoxygenation and antioxidants affect the nitric oxide (NO) synthesis of VSMC. This study tried to investigate the effects of antioxidants on NO production, inducible nitric oxide synthase (iNOS) and the expression of $NF_{\kappa}B$ p65, during the hypoxia-reoxygenation of VSMC cultures. Methods: The VSMCs were primarily cultured from rat aortae, and confirmed by immunoreaction with the antismooth muscle myosin antibody. The condition of the hypoxia was verified by measuring the $PO_2$ and $PCO_2$ of the culture media. The concentrations of nitrite in the culture media were measured by the Griess reaction. Western blottings for the iNOS and $NF_{\kappa}B$ p65 proteins were performed. L-NAME was used as an NOS inhibitor. Vitamins C and E, Glutathione (GSH), lipoic acid and dihydrolipoic acid (DHLA) were used as antioxidants. Results: The iNOS protein was induced in the VSMC by 24 hours of hypoxia, which increased the nitrite in the VSMC culture medium. The reoxygenation profoundly increased the iNOS protein expression and nitrite concentration. The LNAME, vitamins C and E, GSH, lipoic acid and DHLA decreased the nitrite productions during hypoxia and the hypoxia-reoxygenation, whereas, the expressions of the iNOS and $NF_{\kappa}B$ p65 proteins were not influenced. Conclusion: We concluded that hypoxia-reoxygenation induced the iNOS protein, and the subsequent production of NO in the VSMC. The antioxidants and the NOS inhibitor decreased the NO production during the hypoxia-reoxygenation, but did not affect the expressions of the iNOS and $NF_{\kappa}B$ p65 proteins.

Keywords

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