Journal of Korean Society of Forest Science (한국산림과학회지)

Korean Society of Forest Science (한국산림과학회)
권호 표지

Spatial Genetic Structure of Korean Black Raspberry (Rubus coreanus) at Mt. chiak Using I-SSR Markers

I-SSR 標識子를 利用한 치악산 복분자딸기(Rubus coreanus)의 空間分布에 따른 遺傳構造

Kang, Bum-Yong;Hong, Kyung-Nak;Chung, Jae-Min;Hong, Yong-Pyo
강범용;홍경낙;정재민;홍용표

  • Published : 20030000
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Abstract

This study was conducted to analyze the spatial genetic structure of Korean Black Raspberry(Rubus coreanus) a shrubby perennial species, at Seunghwangrim in Mt. Chiak using I-SSR (inter-simple sequence repeats) marker system. Also, it was investigated the spatial pattern of total 60 individuals which was divided into 'Plot I (45 individuals)' within the stand and 'Plot II (15 individuals)' along the brooklet. There were 34 polymorphic and 5 monomophic markers generated from 5 primers, Percentage of polymorphic markers was 87.2%. Shannon's Index showed higher genetic diversity within Plot I (0.579) than Plot II (0.450), and the average was 0.576. Mean distance of pairs between trees was 8.0m in Plot I and 19.8m in Plot II, and maximum distance was 222.6m in Plot I and 278. 0m in Plot II. Aggregation Index in Plot I (0.647) was significant at 0.1% level, which was the clumped distribution, but that of Plot II exhibited the random distribution. The results of spatial autocorrelation analysis revealed that the individuals in Plot I was genetically homogeneous within spatial distance of 20m and the randomness of genetic distribution was from 20m to 60m. However, there was the absence of genetic patchiness in Plot II . For the ex situ conservation of Korean black raspberry in Mt. Chiak, the individuals may be collected with the spatial distance of 20 meters between trees, In directional variogram using genetic distance in Plot I, the increment of spatial distance from South-West to North-East direction was inversely proportional to genetic homogeneity and was coincided with the narrow path in the study site. The anisotrophy of distribution of R. coreanus in Plot I might be resulted from the directionality of seedling dispersal along the narrow path where high intensity of light is provided.

본 연구는 I-SSR(inter-simple sequence repeats) 표지자를 이용하여 덩굴성 낙엽활엽관목 복분자딸기(Rubus coreanus)의 공간적 분포에 따른 유전적 구조를 파악하기 위해서 치악산 성황림내 임내지역의 45개체(Plot I)와 개울가 지역의 15개체(Plot II) 총60개체에 대한 공간분포를 조사하였다. 선발된 4개 I-SSR primer에서 총 39개 I-SSR 증폭물을 얻었으며 5개의 단형성 증폭물을 제외한 34개의 증폭물을 분석에 이용하였다. 평균 다형성 표지자 비율은 87.2%였으며 I-SSR 표지자 다양성(Shannon's Index)은 평균 0.576(Plot I : 0.579, Plot II : 0.450)로 높게 나타났다. 두 개체간 쌍을 이룬 경우에 개체간의 평균거리는 각각 8.0m(Plot I)차 19.8m(Plot II)였고, 가장 멀리 떨어진 두 개체간 거리는 222.6m(Plot I)와 278.0m(Plot II)였다. 또한 조사구내 개체의 분포상태를 나타내는 군집지수는 Plot I의 복분자딸기는 0.647(0.1% 유의수준)로 집중분포를 나타냈으나 Plot II는 임의 분포를 나타냈다. 공간의 자기상관성분석에서 Plot I 지역은 20m이내에 유전적 동질성을 갖는 군락의 크기를 나타냈으며, 20-60m 구간에서는 임의분포를 나타내었다. Plot II 지역은 유전적 군락이 형성되지 않았다. 본 연구결과 현지외 유전자원보존을 위한 개체의 선정은 최소한 20m 이상의 거리를 띄어야 바람직한 것으로조사되었다. Plot I 지역의 복분자딸기의 공간적 위치에 대한 방향성을 파악하기 위해 분산도 분석을 실시한 결과, 임분내 유전적 방향성은 남서-북동 방향으로 거리의 증가에 따라 개체간 유전적 동질성이 계속 감소하는 것으로 나타났으며 임분내 小路의 방향과 일치하였다. 따라서 Plot I 지역의 복분자딸기의 공간적 방향성의 원인은 소로에서의 충분한 수광량에 따른 실생묘의 전파 방향성에 따른 것으로 생각된다.

Keywords

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