Food Science and Biotechnology

Korean Society of Food Science and Technology (한국식품과학회)
권호 표지

Anti-cariogenic Activity of a Bacteriocin Produced by Lactococcus sp. HY 449

Oh, Se-Jong;Lee, Jung-Hee;Kim, Gyung-Tae;Shin, Jung-Gul;Baek, Young-Jin

  • Published : 20030200
⟨ Previous Next ⟩

Abstract

The purpose of this study was to evaluate bacteriocin activity against cariogenic bacteria including Streptococcus mutans. The spot-on-lawn method was used to detennine the antimicrobial activity against indicator strains. A bacteriocin produced by Lactococcus sp. HY 449 inhibited the growth of S. mutans 3065, S. mutans MT 8148, S. mutans CNU, S. sorbrinus ATCC 27607, S. mitis KCTC 3556, and S. vestibularis KCTC 3650. The bacteriocin was partially purified by ammonium sulfate precipitation and hydrophobic interaction chromatography (HIC). Using chromatography with octyl-sepharose CL-4B, higher molecular weight and undesirable molecules were eluted with ammonium sulfate and $H_2O, $ while the bacteriocin was eluted with ethanol. Purified bacteriocin was added to S. mutans MT 8148 cells during logarithmic growth. After 5 hr of incubation, the optical density (O.D. 660 nm) of cells without bacteriocin increased. Whereas the O.D. of cell suspensions decreased in the presence of bacteriocin, indicating that bacteriocin appears to cause cell lysis of tested strains. The addition of bacteriocin statistically showed decrease of plaque formation (P<0.05). The mean weight of plaque in the culture of S. mutans MT 8148 exhibited 82.8 mg/20 mL of M17-5% sucrose broth, whereas the mean weight of plaque was 11.8 mg/20 mL of M 17-5% sucrose broth in the presence of 100 AU/mL bacteriocin. These results suggest that bacteriocin produced by Lactococcus sp. HY 449 is potentially useful in controlling cariogenic bacteria.

Keywords

References

  1. Caries Research v.9 no.1 Metabolism of the polysaccharides of human dental plaque. I. Dextranase activity of streptococci, and the extracellular polysaccharides synthesized from sucrose Dewar, M.D.;Walker, G.J. https://doi.org/10.1159/000260139
  2. J. Am. Dent. Ass. v.87 The potential of antibiotics as caries-control agents Fitzgerald, R.J.
  3. Yakhak Hoeji v.34 The Isolation and evaluation of bioactive components from crude drugs against a cariogenic bacterium, Streptococcus mustans OMZ 176(1) Bae, K.H.;Kim, R.H.;Myung, P.K.;Chung, K.S.;Baek, J.H.
  4. Appl. Environ. Microbiol. v.59 Inhibitory effect of Oolong tea polyphenols on glucosyltransferases of mutans Streptococci Nakahara, K.;Kawabata, S.;Ono, H.;Ogura, K,;Tanaka, T.;Ooshlma, T.;Hamada, S.
  5. Crit. Rev. Food Sci. Nutr. v.38 Probiotic spectra of lactic acid bacteria Naidu, A.S.;Bidlack, W.R.;Clemens, R.A.
  6. Biopolymers v.55 no.1 Posttranslationally modified bacteriocins- The lantibiotics Guder, A.;Wiedemann, I.;Shal, H.G. https://doi.org/10.1002/1097-0282(2000)55:1<62::AID-BIP60>3.0.CO;2-Y
  7. Microbiol. Rev. v.59 Bacteriocins of Grampositive bacteria Jack, R.W.;Tagg, J.R.;Ray, B.
  8. Antimicrobial potential of lactic acid bactena, Bacteriocins of Lactic Acid Bacteria De Vuyst, L.;Vandamme, E.J.
  9. Bacteriol. Rev. v.40 Bacteriocins of gram-positive bacteria Tagg, J.R.;Dajani, A.S.;Wannamaker, L.W.
  10. J. Appl. Bactreiol. v.69 Antibacterial activity of lactic acid bacteria isolated from vacuum-packaged meats Ahn, C.;Stiles, M.E. https://doi.org/10.1111/j.1365-2672.1990.tb01520.x
  11. FEMS Microbiol. Rev. v.12 Genetics of bacteriocins produced by lactic acid bacteria Klaenhammer, T.R.
  12. J. Korean Dairy Technol. Sci. v.19 Characteristlcs of class II bacteriocins produced by lactic acid bacteria Oh, S.
  13. Korean J. Appl. Microbiol Biotechnol. v.22 Isolation of bacteriocin-producing Lactococcus sp. HY 449 and its antimicrobial characteristics Kim, S.K.;Lee, S.J.;Baek, Y.J.;Park, Y.H.
  14. Korean J. Appl. Microbiol. Biotechnol. v.29 Purification and partial amino acid sequence of a bacteriocin produced by Lactococcus sp. HY 449 Oh, S.;Lee, S.J.;Kim, G.T.;Kim, S.K.;Park, Y.H.;Baek, Y.J.
  15. J. Dairy Sci. v.83 Characterization and purification of a bacteriocin produced by Lac.1obacilb.ls addophilus 30SC.: Human isolate for potential use as a probiotic strains Oh, S.;Kim, S.;Worobo, R.W. https://doi.org/10.3168/jds.S0022-0302(00)75169-1
  16. Analytical Biochemistry v.166 no.2 Tricine-sodium dodecyl sulfatepolyacrylannde gel electrophoresis for the separation of proteins III the range from 1 to 100 kDa Schagger, H.;Jagow, G.V. https://doi.org/10.1016/0003-2697(87)90587-2
  17. Archives of Oral Biology v.12 no.12 An in vitro method for assessing the plaque forming ability of oral bacteria McCabe, R.M.;Keyes, P.H.;Howell, A. Jr. https://doi.org/10.1016/0003-9969(67)90200-2
  18. J. Korean Soc. Microbiol. v.35 Effect of Lactococcus factis 1370 on the formation of artificial plaque Chung, J.;Yim, S.Y.;Oh, J.S.