Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Identification of Lactic Acid Bacteria in Kimchi Using SDS-PAGE Profiles of Whole Cell Proteins

  • Kim, Tae-Woon (Institute of Life Science & Biotechnology and Plant Metabolism Research Center, Kyung Hee University) ;
  • Jung, Sang-Hoon (Institute of Life Science & Biotechnology and Plant Metabolism Research Center, Kyung Hee University) ;
  • Lee, Ji-Yeon (Institute of Life Science & Biotechnology and Plant Metabolism Research Center, Kyung Hee University) ;
  • Choi, Sun-Kyu (Institute of Life Science & Biotechnology and Plant Metabolism Research Center, Kyung Hee University) ;
  • SUN-HEE-PARK (Food Microbiology Division, Korea Food and Drug Administration) ;
  • JAE-SUN-JO (Institute of Life Science & Biotechnology and Plant Metabolism Research Center, Kyung Hee University)
  • Published : 2003.02.01
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Abstract

This study was conducted to evaluate the practical usefulness of the sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PACE) fingerprinting of whole cell proteins far the identification of lactic acid bacteria in Kimchi. SDS- PACE of whole cell proteins of the reference strains and lactic acid bacteria isolated from Kimchi yielded differential banding patterns that were highly specific fingerprints, thus making it possible to identify. Identification of the isolates from Kimchi was achieved by comparing the SDS-PAGE fingerprints of isolates to those of reference strains. In addition, the reliability of SDS-PAGE was examined by comparing the results with those of the APL 50 CHL system assay and 16S rRNA gene sequence. SDS-PACE assay showed a different identity to reference strains, while the APL 50 CHL system and 16S rRNA gene sequence could not distinguish a few strains. Therefore, SDS-PAGE of the whole cell proteins is a specific and a reliable method that will be useful for the identification of lactic acid bacteria in Kimchi to the species level, and can be used as an alternative or complementary identification method.

Keywords

References

  1. System Appl. Microbiol. v.17 Comparison of the Lactococcus lactis differential medium (DCL) and SDS-PAGE of whole cell proteins for the identification of Lactococci to subspecies level Descheemaeker P.;B. Pot;A. M. Ledeboer;T. Verrips;K. Kersters https://doi.org/10.1016/S0723-2020(11)80063-9
  2. J. Clin. Microbiol. v.31 Identification of Leuconostoc spp. by analysis of soluble whole-cell protein patterns Elliott J. A.;R. R. Facklam
  3. Kor. J. Appl. Microbiol. Biotechnol. v.27 Dextransucrase activity of Leuconostoc sp. strains isolated from Kimchi Hahn Y. S.;J. Y. Oh
  4. Biotech. Lett. v.22 Use of SDS-PAGE of cell-wall proteins for rapid differentiation of Lactobacillus delbrueckii subsp. lactis and Lactobacillus helveticus Hebert E. M.;R. R. Raya;G. S. de Giori https://doi.org/10.1023/A:1005645422298
  5. J. Microbiol. v.38 Identification and characterization of Leuconostoc gelidum, isolated from kimchi, a fermented cabbage product Kim B. J.;H. J. Lee;S. Y. Park;J. H. Kim;H. U. Han
  6. J. Microbiol. Biotechnol. v.12 Identification and distribution of predominant lactic acid bacteria in Kimchi, a Korean traditional fermented food Kim T. W.;J. Y. Lee;S. H. Jung;Y. M. Kim;J. S. Jo;D. K. Chung;H. J. Lee;H. Y. Kim
  7. Appl. Environ. Microbiol. v.66 Characterization and determination of origin of lactic acid bacteria from a sorghum-based fermented weaning food by analysis of soluble proteins and amplified fragment length polymorphism fingerprinting Kunene N. F.;I. Geornaras;A. Holy;J. W. Hastings https://doi.org/10.1128/AEM.66.3.1084-1092.2000
  8. Kor. J. Appl. Microbiol. Biotechnol. v.20 Microfloral changes of the lactic acid bacteria during Kimchi fermentation and identification of the isolates Lee C. W.;C. Y. Ko;D. M. Ha
  9. Kor. J. Food Sci. Technol. v.30 Acid tolerance of Lactobacillus brevis isolated from Kimchi Lee K. S.;Y. S. Shin;C. H. Lee
  10. Kor. J. Microbiol. v.27 Reevaluation of isolation and identification of gram-positive bacteria in Kimchi Lim C. R.;H. K. Park;H. U. Han
  11. Kor. J. Food Sci. Technol. v.16 Effect of temperature and salt concentration on Kimchi fermentation Mheen T. I.;T. W. Kwon
  12. J. Gen. Microbiol. v.139 Identification and classification of Lactobacillus acidophilus, L. gasseri and L. Johnsonii strains by SDS-PAGE and rRNA-targeted oligonucleotide probe hybridization Pot. B. C.;Hertel W. Ludwig;P. Descheemaeker;K. Kersters;K. H. Schleifer https://doi.org/10.1099/00221287-139-3-513
  13. Chemical Methods in Bacterial Systematics Analysis of electrophoretic whole-organisms protein fingerprints Pot B.;P. Vandamme;K. Kersters
  14. Kor. J. Food Sci. Technol. v.22 Lactic acid bacteria isolated from fermenting Kimchi and their fermentation of Chinese cabbage juice Shim S. T.;K. H. Kyung;Y. J. Yang
  15. Kor. J. Food Sci. Technol. v.28 Changes of chemical composition and microflora in commercial Kimchi Shin D. H.;M. S. Kim;J. S. Han;D. K. Lim;W. S. Bak
  16. Mycolog. Res. v.93 Restriction fragment length polymorphism in mitochondrial DNAs of Armillaria Smith M. L.;J. B. Anderson https://doi.org/10.1016/S0953-7562(89)80151-0
  17. Kor. J. Food Sci. Technol. v.27 Identification of psychrotrophic lactic acid bacteria isolated from Kimchi So M. H.;Y. B. Kim
  18. Int. J. Food Microbiol. v.36 Lactic acid bacteria of food and their current taxonomy Stiles M. E.;W. H. Holzapfel https://doi.org/10.1016/S0168-1605(96)01233-0
  19. System Appl. Microbiol. v.17 The combined use of whole cell protein extracts for the identification (SDS-PAGE) and enzyme activity screening of lactic acid bacteria isolated from traditional Greek dairy products Tsakalidou E.;E. Manolopoulou;E. Kabaraki;E. Zoidou;B. Pot;K. Kersters;G. Kalantzopoulos https://doi.org/10.1016/S0723-2020(11)80062-7