Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Isolation of Nitrogen-Fixing Bacteria from Gramineous Crops and Measurement of Nitrogenase Activity

벼과식물로부터 질소고정균의 분리와 Nitrogenase 활성 측정

  • 최은화 (경북대학교 미생물학과) ;
  • 이상은 (경북대학교 미생물학과) ;
  • 윤기순 (경북대학교 생물공학과) ;
  • 권덕기 (경북대학교 생물공학과) ;
  • 손재근 (경북대학교 농학과) ;
  • 박승환 (한국생명공학연구원 유전체연구센터) ;
  • 한명숙 (경북대학교 미생물학과) ;
  • 김사열 (경북대학교 미생물학과)
  • Published : 2003.03.01
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Abstract

For researching nitrogen-fixing bacteria associated with gramineous crops, we collected growing roots of rices, wheats, oats, barleys, ryes, and maizes at 19 sites of southern Korean peninsula. Endophytes and free living bacteria were isolated from those crop roots. Sixty-three isolates were classified on the basis of different morphology, size, color, host of colony, and the 16S rDNAs sequence. The analyses of PCR amplification for nifH gene and nitrogenase activity assay, revealed that all isolates contained nitrogen-fixing abilities. In addition, most of them have cellulase activity which is one of the common features of endophytic bacteria from plant.

벼과식물과 관련된 질소고정균을 분리하기 위하여 한반도 남부지방의 19지역에서 쌀, 밀, 귀리, 보리, 호밀, 옥수수 등을 채집하여 이들의 뿌리로부터 free living bacteria와 endophyte를 분류하였다. Colony의 형태, 크기, 색깔 등과 16S rDNA 염기서열을 근거로 63종으로 분류하였다. nifH 유전자 염기서열 결정과 nitrogenase 활성 측정을 통하여 분리균주 모두 질소고정 능력이 있음을 확인하였다. 그리고 식물에서 분리한 내생균의 특징 중 하나인 cellulase활성이 대부분의 분리 균주에서 확인되었다.

Keywords

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