Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Adventitious Root Cultures of Panax ginseng C.V. Meyer and Ginsenoside Production through Large-Scale Bioreactor System

  • Hahn, Eun-Joo (Research Center For the Development of Advanced Horticultural Technology, Chungbuk National University) ;
  • Kim, Yun-Soo (Research Center For the Development of Advanced Horticultural Technology, Chungbuk National University) ;
  • Yu, Kee-Won (Research Center For the Development of Advanced Horticultural Technology, Chungbuk National University) ;
  • Jeong, Cheol-Seung (Research Center For the Development of Advanced Horticultural Technology, Chungbuk National University) ;
  • Paek, Kee-Yoeup (Research Center For the Development of Advanced Horticultural Technology, Chungbuk National University)
  • Published : 2003.03.01
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Abstract

The adventitious root of Panax ginseng C.A. Meyer is regarded as an efficient alternative to cell culture or hairy root culture for biomass production due to its fast growth and stable metabolite production. To determine optimal culture conditions for the bioreactor culture of ginseng roots, experiments have been conducted on physical and chemical factors such as bioreactor type, dissolved oxygen, gas supply, aeration, medium type, macro- and micro-elements, medium supplement during culture period, sucrose concentration, osmotic agents, medium pH and light. Elicitation is a key step to increase ginsenoside accumulation in the adventitious roots but biomass growth is severely inhibited by elicitor treatment. To obtain high ginsenoside content with avoiding biomass decrease, we applied two-stage bioreactor culture system. Ginseng adventitious roots were cultured for 40 days to maximize biomass increase followed by elicitation for 7 days to enhance ginsenoside accumulation. We also experimented on types and concentrations of jasmonate to determine optimal elicitation methods. In this paper, we discussed several factors affecting the root propagation and ginsenoside accumulation. Based on the results obtained from previous experiments we have established large-scale bioreactor system (1 ton-10 ton) for the efficient production of ginseng adventitious roots and bioactive compounds including ginsenoside. Still, experiments are on going in our laboratory to determine other bioactive compounds having effects on diet, high blood pressure, DPPH elimination and increasing memories.

Keywords

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