Journal of Nutrition and Health

The Korean Nutrition Society (한국영양학회)
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Effects of Liquid Culture of Agaricus blazei Murill on Lipid Metabolism and Enzyme Activities in Rats Fed High Fat Diet

신령버섯 균사체 액체배양액이 고지방 식이를 급여한 흰쥐의 지질대사 및 효소활성에 미치는 영향

  • 이현정 (신라대학교 생명과학과) ;
  • 고진복 (신라대학교 생명과학과)
  • Published : 2003.05.01
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Abstract

This study was performed to investigate the effects of liquid culture of Agaricus blazei on the lipid metabolism and enzyme activities in growing male rats. Sprague-Dawley rats were given four different types of diets for a succeeding period of 5 weeks, respectively: a normal diet group (7% corn oil), a high fat diet group (7% com oil + 15% lard), a 20 or 30% Agaricus diet groups (high fat diet + 20 or 30% Agaricus in water) according to the levels of Agaricus supplementation. The body weight gains, food intake, food efficiency ratios, and hepatic, kidney, spleen and pancreas weights of the rats fed 20 or 30% Agaricus diets were similar to those of the rats fed high fat diet. The epididymal fat pad weight of the rats fed high fat diet and 20 or 30% Agaricus diets were significantly higher than that of the rats fed normal diet. The concentrations of serum triglyceride, total cholesterol, LDL-cholesterol and HDL-cholesterol, and the activity of glutamic pyruvic transaminase in the rats fed 30% Agaricus diet were significantly lower than those in the rats 114 high fat diet. But the concentrations of hepatic total cholesterol and triglyceride of rats fed the 20 or 30% Agaricus diets were similar to those of rats fed the high fat diet. The HDL-oholesterol/total-cholesterol ratio of the rat fed 30% Agaricus diet was significantly higher than that of the rats fed high fat diet, The activity of glutamic oxaloacetic transaminase in the rats fed 20 or 30% Agaricus diets were similar to those in the rats fed high fat diet. There were no differences in the concentrations of total protein, hemoglobin and glucose, and the activities of alkaline phosphatase, and the atherogenic index in the serum among the experimental groups. These results showed that the 30% Agaricus diet feeding decreased the total cholesterol, the triglyceride and the LDL-cholesterol, and increased the HDL-oholesterol/total cholesterol ratio in serum of rats.

신령버섯 균사체 배양액이 고지방식이를 섭취한 숫쥐의 성장률, 장기무게, 지질 농도, 단백질 농도 및 효소활성에 미치는 영향을 조사하고자, 생후 7주령의 흰쥐 (255.3 $\pm$ 20.2 g)에 표준식이를 급여한 정상군, 표준식이에 15% 돈지를 첨가한 식이를 급여한 고지방군, 고지방 식이에 신령버섯의 균사체 배양액을 음료수에 20% 및 30%로 혼합 급여한 군 (20% 및 30% 신령버석군) 등 4군으로 나누어 5주간 사육한 결과는 다음과 같다. 실험동물의 체중증가량, 식이섭취량 및 식이효율, 그리고 간, 신장, 비장 및 췌장의 무게는 고지방군과 20% 및 30% 신령버섯군이 비슷한 수준을 유지하였다. 부고환지방은 정상군에 비하여 고지방군 및 신령버섯군들이 유의하게 증가되어 신령버섯 섭취에 따른 감소효과는 나타나지 않았다. 혈청의 총 콜레스테롤, 중성지질, LDL-콜레스테롤, HDL-콜레스테를 농도 및 glutamic pyruvic transaminase 활성은 고지방군에 비해 30%신령버섯군이 유의하게 감소되었다. 간의 콜레스테롤과 중성지질 농도는 고지방군과 20% 및 30% 신령버섯군이 비슷한 수준으로 감소효과가 나타나지 않았다. 총 콜레스테롤에 대한HDL-콜레스테롤의 비율은 고지방군에 비해 30%신령버섯군이 유의하게 증가되었다. 혈청의 단백질, 혈색소 및 혈당 농도, 동맥경화지수와 alkaline phosphatase 활성은 정상군과 각 실험군이 비슷한 수준을 유지하였다. 혈청의 glutamic oxaloacetic transaminase 활성은 고지방군과 20% 및 30% 신령버섯군이 비슷한 경향이었다. 이상의 결과로 보아 고지방식이를 급여한 흰쥐에 신령버섯 균사체 배양액을 30% 급여시 체중증가량과 장기 무게는 정상수준을 유지하였고, 혈청의 총 콜레스테롤, 중성지질 및 LDL-콜레스테를 농도를 낮추고, 총 콜레스테롤에 대한 HDL-콜레스테롤의 비율을 증가시키는 효과가 나타났다.

Keywords

References

  1. Kubo K, Nanba H. The effect of Maitake mushrooms on liver and serum lipids. Altern Ther Health Med 2: 62-69, 1996
  2. Liu F, Ooi VE, Chang ST. Free radical scanvenging activities of mushroom polysaccharide extracts. Life Sci 60: 763-766, 1997 https://doi.org/10.1016/S0024-3205(97)00004-0
  3. Sung JM, Yoo YB, Cha DY. Mushroom. Kyohaksa. Seoul, pp.3-10, 1998
  4. Mizuno T, Hagiwara T, Nakamura T, Ito H, Shimura K, Sumiya T. Antitumor activity and some properties of water soluble polysaccharides from the fruiting body of Agaricus blazei Muril, Agric Biol Chem 54: 2889-2896, 1990 https://doi.org/10.1271/bbb1961.54.2889
  5. Chang HL, Chao GR, Chen CC, Mau JL. Non-volatile taste components of Agaricus blazei, Antrodia camphorata and Cordyceps militaris mycelia. Food Chemistry 74: 203-207, 2001 https://doi.org/10.1016/S0308-8146(01)00127-3
  6. Sugiyama K, Saeki S, Ishiguro Y. Hypercholesterolemic activity of ningyotake (Polyporus confleuens) mushroom in rats. J Jpn Soc Nutr Food Sci 45: 265-270, 1992 https://doi.org/10.4327/jsnfs.45.265
  7. Yoshiaki F, Hidekazu K, Koichi O, Ryo S, Takusaburo E. Tumoricidal activity of high molecular weight polysaccharides derived from Agaricus blazei via oral administration in the mouse tumor model. Nippon Shokuhim. Kagaku Kaishi 45: 246-252, 1998 https://doi.org/10.3136/nskkk.45.246
  8. Itoh H, Amano H, Noda H. Inhibitory action of a (1→6)-β-D-glucan-protein complex isolated from Agaricus blazei Muril on metha fibrosarcoma-bearing mice and its antitumor mechanism. Jpn J Pharmacol 66: 265-271, 1994 https://doi.org/10.1254/jjp.66.265
  9. Mizuno T, Inagaki R, Kanao T, Hagiwara T. Antitumor activity and some properties of water-insoluble hetero-glucans from 'Himematsutake' the fruiting body of Agaricus blazei Muril. Agric Biol Chem 54: 2897-2905, 1990 https://doi.org/10.1271/bbb1961.54.2897
  10. Kawagish H, Inagaki R, Kanao T. Fraction and antitumor activity of the water-insoluble residue of Agaricus blazei fruiting bodies. Carbohydrate Research 186: 267-273, 1989 https://doi.org/10.1016/0008-6215(89)84040-6
  11. Menoli RCRN, Mantovani MS, Ribeiro LR, Speit G, Jordao BQ. Antimutagenic effects of the mushroom Agaricus blazei Murrill extracts on V79 cells. Mutation Research 496: 5-13, 2001 https://doi.org/10.1016/S1383-5718(01)00227-3
  12. Dong Q, Yao J, Yang XT, Fang JN. Structural characterization of af water-soluble β-D-glucan from fruiting bodies of Agaricus blazei Murr. Carbohydrate Research 337: 1417-1421, 2002 https://doi.org/10.1016/S0008-6215(02)00166-0
  13. Kawagish H, Kanao T, Inagaki R, Mizuno T, Shimura K, Ito H, Hagiwara T, Nkamura T. Formolysis of a potent antitumor (1-6)-棺-D-glucan-protein complex from Agaricus blazei fruiting bodies and antitumor activity of the resulting products. Carbohydrate Polymers 12: 393-403, 1990 https://doi.org/10.1016/0144-8617(90)90089-B
  14. Choi JM, Koo SJ. Effects of β-glucan from Agaricus blazei Murill on blood glucose and lipid composition in db/db mice. Korean J Food Sci Technol 32: 1418-1425, 2000
  15. Lee JS, Lee KH, Jeong JH. Effects of extract of Pueraria radix on lipid metabolism in rats fed high fat diet. J Korean Soc Food Sci Nutr 28: 218-224, 1999
  16. Koh JB. The effects of Cordyceps militaris on lipid metabolism, protein levels and enzyme activities in rats fed a high fat diet. Korean J Nutrition 35: 414-420, 2002
  17. Reeves PG, Nielsen FH, Fahey GC. AIN-93 purified diets for laboratory rodents final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet. J Nutr 123: 1939-1951, 1993
  18. Medical Laboratory. Technology and Clinical Pathology, 2th ed. WB. Saunders Co. philadelphia, pp.673, 1969
  19. Davidson I, Henry JB. Todd-Sanford clinical diagnosis by laboratory methods, 13th ed. WB. Saunders Co. Philadelphia. pp.73-75, 1966
  20. Warnick GR, Knoopp RH, Fitzpatrick V, Branson L. Estimating low density lipoprotein cholesterol by the Friedewald equation is adequate for classifying patient on the basis of nationally recommended cutpoints. Clin Chem 36: 15-19, 1990
  21. Schectman G, Patsches M, Sasse EA. Variability in cholesterol of measurements: comparison of calculated and direct LDL cholesterol determinations. Clin Chem 42: 732-737, 1996
  22. Friedwald WT, Levy RI, Fedreicson DS. Estimation of the concentration of low density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem 18: 499-508, 1979
  23. Haglund O, Loustarinen R, Wallin R, Wibell I, Saldeen T. The effect of fish oil on triglycerides, cholesterol, fibrinogen and malondialdehyde in humans supplemented with vitamin. Eur J Nutr 121: 165-172, 1991
  24. Folch J, Lees M, Stanley GSH. A simple method for the isolation and purification of total lipid from animal tissues. J Biol Chem 226: 497-509, 1957
  25. Frings CS, Dunn RT. A colorimetric method for determination of total serum lipid based on the sulfo-phospho-vanillin reaction. Am J Clin Path 53: 89-91, 1970
  26. Kim CO, Kang SA. Effect of high fat and high carbohydrate diet on serum leptin and lipids concentration in rat. Korean J Nutrition 34: 123-131, 2001
  27. Park YJ, Park YJ. Effect of high fat and high cholesterol diet on kidney function. Korean J Nutrition 30: 187-194, 1997
  28. Kim GJ, Kim HS, Chung SY. Effects of varied mushroom on lipid compositions in dietary hypercholesterolemic rats. J Korean Soc Food Sci Nutr 21: 131-139, 1997
  29. Song CW, Hwang HS, Han SS. Studies on the basic data of Ktc: SD rats with age. Korean J Lab Ani Sci 6: 33-43, 1990
  30. Flora SJ, Singh S, Tandon S. Combined effects of thiamin and calcium disodium versenate on lead toxicity. Life Science 38: 67-75, 1985 https://doi.org/10.1016/0024-3205(86)90276-6
  31. Chandra RK. Cell-mediated immunity in genetically obese mice. Am J Clin Nutr 33: 13-19, 1980
  32. Kim GH, Han HK. The effect of mushroom extracts on carbon tetrachloride-induced hepatotoxicity in rats. J Korean Soc Food Sci Nutr 27: 326-332, 1998
  33. Koh JB, Choi MA. Effect of Cordyceps militaris on lipid metabolism in rats fed cholesterol diet. Korean J Nutrition 34: 265-270, 2001
  34. Sugiyama K, Akachi T, Yamakawa A. Hypocholesterolemic action of eritadenine Is mediate by a modification of hepatic phospholipid metabolism in rat. J Nutr 125: 2134-2140, 1995
  35. Kim BK, Shin GG, Jeon BS, Cha JY. Cholesterol-lowering effect of mushrooms power in hyperlipidemic rats. J Korean Soc Food Sci Nutr 30: 510-515, 2001
  36. Kabir Y, Kimura S, Tamura T. Dietary effects of Ganoderma lucidum mushroom on blood pressure and lipid levels in spontaneously hypotensive rats (SHR). J Nutr Sci Vitaminol 34: 433-438, 1988 https://doi.org/10.3177/jnsv.34.433
  37. Cheung PCK. The hyporcholesterolemic effect of extracellular polysaccharide from the submerged fermentation of mushroom. Nutr Res 16: 1953-1957, 1996 https://doi.org/10.1016/S0271-5317(96)00218-7
  38. Ebihara K, Schnceman BO. Interaction of bile acids, phospholipids, cholesterol and triglycerides with dietary fiber inthe small intestine of rats. J Nutr 119: 1100-1106, 1989
  39. Kinosian B, Glick H, Preiss L, Puder KI. Cholesterol and coronary heart disease: predicting risk in men by changes in levels and ratios. J Invest Med 43: 443-450, 1995
  40. Kailash P. Reduction of serum cholesterol and hypercholesterolemic atherosclerosis in rabbits by secoisolariciresinol diglucoside isolated from flaxseed. Circulation 99: 1355-1362, 1999 https://doi.org/10.1161/01.CIR.99.10.1355
  41. Jung MY, Nam SM, Kang IJ, Kim SJ, Chung CK. Biochemical and histological effects of Phellinus linteus methanol extract on liver lipid metabolism of rats fed CCl4 and high fat. J Korean Soc Food Sci Nutr 30: 331-337, 2001
  42. Kim HS, Park YS, Kim CI. Changes of serum lipid profiles after eating Lycil fructus in rats fed high fat diet. Korean J Nutrition 31: 263-271. 1998