Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Diversity and Antibacterial Activity of Lactic Acid Bacteria Isolated from Kimchi

김치로부터 분리한 젖산균의 다양성과 항균성

  • Published : 2003.06.01
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Abstract

This study was carried out to investigate the isolation, identification, and antibacterial activity of lactic acid bacteria related to kimchi fermentation. Diluted kimchi soup was plated on the MRS agar media with CaCO$_3$ and incubated at $25^{\circ}C$ for 2 days. A total of 27 strains of lactic acid bacteria from various indigenous, spontaneously fermented vegetables (kimchi) were isolated. Combined methods of Bergey's manual of systematic bacteriology, BPB media analysis and 16S rDNA sequence analysis were applied for identification, however, their results did not coincide in several cases. Isolated lactic acid bacteria could be classified by the 16S rDNA sequence analysis as Leuconostoc mesenteriodes, Leu. carnosum, Lactobacillus curvatus, Lac. pentosus, Weisselia kimchi, W. cibaria, and Pediococcus pentosaceus. Leu. carnosum has not been reported in kimchi lactic acid bacteria. In addition, antibacterial activities of the isolates were tested with Bacillus subtilis, Escherichia coli, Salmonella enteritidis, S. paratyphica, S. typhi, Staphylococcus aureus, Shigella boydii, and S. sonnei. Some of isolates showed significant antibacterial activities to those pathogens.

본 연구는 김치로부터 분리한 젖산균의 다양성과 항균성을 조사하기 위하여 수행되었다. 희석한 김치 국물을 CaCO$_3$가 첨가된 MRS 고체 배지에 도말 한 후, $25^{\circ}C$에서 2일 동안 배양하여 여러 가지 김치로부터 27가지의 젖산균을 분리하였다. 이렇게 분리한 젖산균들을 Bergey 분류법과 BPB 배지를 통한 생화학적인 방법과 16S rDNA PCR을 이용한 분자 생물학적인 방법으로써 분석하였다. 그러나 이들 방법으로 분석한 결과가 일치하지 않은 경우가 많았다. 이들 분리 젖산균은 16S rDNA 부분 염기서열을 결정한 결과 Leuconostoc mesenteriodes(11균주), Leu. carnosum(3균주), Lactobacillus curvatus(8균주), Lac. pentosus(2균주), Weisselia kimchi(1균주), W. cibaria(1균주), Pediococcus pentosaceus(1균주) 등의 것과 각각 매우 유사한 것으로 분석되었다. 이들중 Leu. carnosum은 이전에 김치에서 분리되었다고 보고된 바가 없다. 그리고 이들 김치에서 분리 동정한 균들의 항균작용 활성을 검사 해 본 결과 균주에 따라서 활성의 정도는 다르게 나타났으나 Bacillus subtilis, Escherichia coli, Salmonella enteritidis, S. paratyphica, S. typhi, Staphylococcus aureus, Shigella boydii, S. sonnei 등에 대해 뚜렷한 항균효과를 나타내었다.

Keywords

References

  1. Cultured Dairy Products v.6 Antibacterial activity associated with Bifidobacterium Anand,S.K.;R.A.Srinirasan;L.K.Rao
  2. J. Bacteriol. v.62 Studies of lysogenesis. I. The mode of phage libration by lysogenic Escherichia coli Bertani,G.
  3. J. Appl. Microbiol. v.93 Characterization of a bateriocin produced by newly isolated Bacillus sp. Strain 8 A. Bizani,D.;A.Brandelli
  4. Crit. Rev. Food Sci. Nut. v.34 Biochemical, microbiological, and nutritional aspects of Kimchi Cheigh,H.C.;K.Y.Park
  5. J. Microbiol. Biotechnol. v.12 Reevaluation of the change of Leuconostoc sp. and Lactobacillus plantarum by PCR during Kimchi fermentation Choi,J.Y.;M.K.Kim;J.H.Lee
  6. J. Appl. Bacteriol. v.23 A medium for the cultivation of Lactobacilli Deman,J,C,;M.Rogosa;M.E.Sharpe
  7. Appl. Environ. Microbiol. v.64 Comparative studies of class a bacteriocin of lactic acid bacteria Eijsink,V.G.H.;M.Skeie;P.H.Middelhoven;M.B.Brurberg;I.F.Nes
  8. J. Basic Sci. Institute (Inha Univ.) v.12 Differential counts of lactic acid bacteria genera on bromphenol blue medium Han,H.U.;H.K.Park
  9. BERGEY's manual of systematic bacteriology.(9th ed.) John,G.H.;R.K.Noel;H.S.Sneath;T.S.James;T.W.Stanely
  10. Kor. J. Microbiol. Biotechnol v.23 Strain improvement of Leuconosyoc mesenteroides for Kimchi fermentation and effect of staeter Kang,S.M.;W.S.Yang;Y.C.Kim;E.Y.Jung;Y.G.Han
  11. J. Microbiol. v.39 Isolation of dextran-producing Leuconostoc lactis from Kimchi Kim,B.J.;B.H.Min;J.H.Kim;H.U.Han
  12. J. Microbiol. v.38 Identification and characterization of Leuconstoc gelidum, isolated from Kimchi, a fermented cabbage product Kim,B.J.;H.J.Lee;S.Y.Park;J.H.Kim;H.U.Han
  13. Kor. J. Microbiol. Biotechnol. v.30 Characteristics of the bacteriocin from Lactobacillus sp. Oh-B3 Kim,D.S.
  14. J. Kor. Soc. Food Sci. Nutr. v.27 Antimicrobial effect of ethanol extracts from spices against Lactobacillus plantarum and Leuconostoc mesenteriodes isolated from Kimchi Kim,O.M.;M.K.Kim;S.O.Lee;K.R.Lee;S.D.Kim
  15. Biochimie v.70 Bacteriocin of lactic acid bacteria Klaenhammer,T.R.
  16. J. Microbiol. Biotechnol. v.12 Bacteriocin produced by Pediococcus sp. in Kimchi and its characteristics Kwon,D.Y.;M.S.Koo;C.R.Ryoo;C.H.Kang;K.H.Min;W.J.Kim
  17. Kor. J. Microbiol. Biotechnol. v.20 Microfloral changes of the lactic acid bacteria during Kimchi fermentation and identification of the isolates Lee,C.W.;C.Y.Ko;D.M.Ha
  18. FEMS Microbiol. Lett. v.193 Multiplex PCR-based detection of Leuconostoc spercies Lee,H.J.;S.Y.Park;J.H.Kim
  19. J. Kor. Soc. Food Sci. Nutr. v.25 Selective media for isolation and enumeration of lactic acid bacteria from Kimchi Lee,M.K.;W.S.Park;K.H.Kang
  20. Kor. J. Microbiol. Biotechnol. v.25 Viability in Artificial gastric and bile juice and antimicrobial activity of some lactic acid bacteria isolated from Kimchi Lee,S.H.;M.J.No
  21. Kor. J. Microbiol. Biotechnol. v.30 Identification of the cell-envelope proteinase of lactic acid bacteria isolated from Kimchi Lee,Y.J.;J.Y.Choi;H.J.Lee;H.C.Jang;J.H.Kim;D.K.Chung;Y.S.Kim;S.M.Kim;J.H.Lee
  22. J. Appl. Microbiol. v.86 New types of antimicrobial produced by Lactobacillus plantarum Niku-Paavola,M.L.;A.Laitila;T.Mattila-Sandholm;A.Haikara
  23. Kor. J. Microbiol. Biotechnol. v.27 Isolation and characterization of lactic acid bacteria producing antimutagenic substance Korean Kimchi Rhee,C.H.;H.D.Park
  24. J. Dairy Sci. v.37 The effect of lactose crystallization on protein stability in frozen concentrated milk Tumerman,L.;H.Farm;K.W.Conely
  25. J. Dairy Sci. v.37 The effect of lactose crystallization on protein stability in frozen concentrated milk Tumerman,L.;H.Farm;K.W.Conely