Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Pharmacological Effect of Hawangyeonhaedoktang on Experimental Triglyceride Accumulated HepG2 Cells

실험적 중성지질 축적 HepG2세포에 미치는 황련해독탕의 약리적 효과

  • Published : 2003.06.01
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Abstract

The pharmacological effect of Korean-Chinese traditional herbal medicine, Hawangyeonhaedoktang (HT) on experimentally induced-triglyceride accumulation in cultured human hepatocyte HepG2 cells was studied. HePG2 cells were cultured in the Dulbecco's modified Eagle's (DME) medium without (Control medium) or with HT (0.5 mg/mL and 5.0 mg/mL) containing 1 mM oleate, 0.2% bovine serum albumin (BSA), and glucose 4.5 mg/mL for 6 and 24 hours in experiment I and 2 mM oleate, 0.5% BSA, and glucose 4.5 mg/mL for 6, 24 and in hours in Experiment II or 1 and 3 hours in Experiment III. Oleate ［$^{14}$ C］(0.5 $\mu$Ci/mL medium) added as a radioactive lipid precursor in the experiment I. In the experiment I, the intracellular triglyceride concentration was decreased remarkably during incubation for 6 and 24 hours, in a dose-dependent manner. At the same time, HT caused a decrease in the incorporation of ［$^{14}$ C］ oleate into intracellular triglyceride fraction and the secretion of triglyceride labeled with ［$^{14}$ C］ oleate into medium. In the experiment II and III compared to experiment I, the triglyceride accumulation in HepG2 cells was occurred, and HT prevented the accumulation of triglyceride during incubation for 24 and 48 hours. This result suggest that HT prevent the triglyceride accumulation in human hepatocytes by its inhibiting action on the intercellular triglyceride biosynthesis.

사람 간 배양세포 유래 HePG2 세포에 영양성분을 이용하여 실험적으로 중성지질을 축적시킨 상태에서 한방 황련해독탕(HT)의 약리적 효과를 검토하였다. 간세포는 1 mM oleate, 0.2% BSA, glucose 4.5 mg/mL 및 HT 무첨가(대조구) 또는 첨가한(0.5 mg/mL 및 5.0 mg/mL) DME배지에서 6시간 및 24시간 배양하였으며(실험 I), 이때 황련해독탕을 첨가한 배양액에는 ［$^{14}$ C］-oleate (0.5 $\mu$Ci/mL medium)를 동시에 첨가하여 중성지질 획분에의 동위원소 추적실험을 하였다. 또한, 간세포에 2 mM oleate, 0.5% BSA, glucose 4.2mg/mL 및 HT 무첨가(대조구) 또는 첨가한(0.5 mg/mL 및 5.0 mg/mL) DME배지에서 6, 24및 48시간 배양(실험 II) 또는 1 및 3시간 배양(실험 III)하였다. 실험 I에서 세포내 중성지질 농도는 HT첨가 6 및 24시간 배양에서 약물농도첨가 의존적으로 감소하였다. 이때 HT첨가시 ［$^{14}$ C］-oleate 동위원소는 세포내 중성지질 획분의 추적량과 세포외 분비량이 현저하게 감소하였다. 실험 I조건에 비해 고농도의 영양성분을 첨가시킨 실험 II 및 III 조건에서 세포내 중성지질 축적이 확인되었으며, 이 때 HT 첨가 24 및 48시간 배양에서는 세포내 중성지질 축적이 현저히 억제되는 것으로 나타났다. 이상의 결과에서, 본 실험에 사용된 한방 황련해독탕은 사람 간배 양 HePG2 세포내 중성 지질 합성을 저하시켜 중성지질 축적을 억제시킴으로써 지방간 개선효과를 발휘한 것으로 시사되었다.

Keywords

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