KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Catalase Production by Membrane Process for Treatment of Industrial Wastewater Containing Hydrogen Peroxide

막분리 공정을 이용한 과산화수소 함유 폐액처리용 카탈라제 생산

  • Published : 2003.06.01
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Abstract

This study aims to develop an economic process for the treatment of industrial wastewater containing hydrogen peroxide by using catalase. Core process is characterized by two membranes; microfiltration membrane and ultrafiltration membrane with different molecular cut-offs. Optimum dilution ratio of Aspergillus niger molds 개 buffer solution is 1:5. The final recovery yield of the enzyme is over 90% using the process. The enzyme solution shows the optimum temperature of 4$0^{\circ}C$ and pH range of 5-8.

본 연구는 Aspergillus niger 폐포자를 이용하여 과산화수소를 함유한 폐액처리용 효소제조 공정개발에 그 목표가 있다. 본 카탈라제 (catalase) 생산 공정은 미세여과(0.2 $\mu\textrm{m}$ microfiltration)와 한외여과 (300 KDa, 50 KDa, ultrafiltration)의 순차적인 막 여과 방식을 통하여 효소액을 회수하는 것을 특징으로 한다. 초기 폐포자와 완충액의 희석비는 1 : 5였으며 막분리 공정의 수율은 90% 이상이었다. 얻어진 카탈라제 용액의 최적 온도는 4$0^{\circ}C$, 최적 PH는 5-8 영역이었다.

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References

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