Restorative Dentistry and Endodontics

The Korean Academy of Conservative Dentistry (대한치과보존학회)
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PCR-based Identification of Eubacteirum species in endodontic infection

감염 근관에서 중합효소연쇄반응법을 이용한 Eubacterium 균종의 동정

  • Kum, Kee-Yeon (Yonsei Dental College, Department of Conservative Dentistry) ;
  • Fouad, A.F. ( Oral Science Research Center, UCHC Endo, CT, USA)
  • 금기연 (연세대학교 치과대학 보존학교실) ;
  • Published : 2003.05.01
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Abstract

Asaccharolytic Eubacterium균종은 감염 근관에서의 높은 발생 빈도와 독성으로 인해 최근 많이 연구되어지고 있다. 본 연구는 24명의 환자의 감염 근관에서 얻은 22개의 PCR 산물로부터 Eggerthella lenta를 포함한 Eubacterium 균종의 빈도 및 환자의 임상 증상이나 당뇨와의 상관성을 조사한 후 얻은 자료를 토대로 다음과 같은 결과를 얻었다. 1. 22개의 표본 중에서 16개(73%)가 Eubacterium균종을 포함하고 있었으며 이 중 9개의 시편에서 Eubacterium infirmum이 검출되었다. 2. Eggerthella lenta는 어떤 시편에서도 발견되지 않았다. 3, Odds ratio analysis 결과 Eubacterium infirmum은 당뇨병과의 높은 상관성을 보여주었다(OR=9.6, P=0.04).

Keywords

References

  1. Wade WG. Lewis MA, Cheeseman SL, Absi EG and Bishop PA. An unclassified Eubacterium taxon in acute dento-alveolar abscess. J Med Microbiol 40: 115-117. 1994 https://doi.org/10.1099/00222615-40-2-115
  2. Wade WG, The role of Eubacterium species in periodontal disease and other oral infections. Mierob Ecol Health Dis 9:367-370, 1996 https://doi.org/10.3109/08910609609166480
  3. Sundqvist G, Associations between microbial species in dental root canal infections. Oral Microbiol Immunol 7:257-262, 1992 https://doi.org/10.1111/j.1399-302X.1992.tb00584.x
  4. Wasfy MO. McMahon KT. Minah GE and Falkler WA. Jr. Microbiological evaluation of periapical infections in Egypt. Oral Microbiol Immunol 7:100-105, 1992 https://doi.org/10.1111/j.1399-302X.1992.tb00517.x
  5. Yoshida M. Fukushima H. Yamamoto K. Ogawa K. Toda T and Sagawa H. Correlation between clinical symptoms and microorganisms isolated from root canals of teeth with periapical pathosis. J Endod 13:24-28. 1987 https://doi.org/10.1016/S0099-2399(87)80088-2
  6. Sundqvist G, Johansson E and Sjogren U. Prevalence of black-pigmented bacteroides species in root canal infections. J Endod 15: 13-19. 1989 https://doi.org/10.1016/S0099-2399(89)80092-5
  7. Sjogren U, Figdor D, Persson Sand Sundqvist G. Influence of infection at the time of root filling on the outcome of endodontic treatment of teeth with apical periodontitis. Int Endod J 30:297-306. 1997 https://doi.org/10.1046/j.1365-2591.1997.00092.x
  8. Kageyama A, Benno Y and Nakase T. Phylogenetic evidence for the transfer of Eubacterium lentum to the genus Eggerthella as Eggerthella lenta gen. nov .. comb. nov. Int J Syst. Bacieriol 49: 1725-1732. 1999 https://doi.org/10.1099/00207713-49-4-1725
  9. Fouad AF. Barry J. Caimano M. Clawson C. Zhu Q. Carver R. Hazlett K and D. RJ. PCR-based identification of bacteria in endodontic infections. J Clin Micro 40:1491-1501. 2002 https://doi.org/10.1128/JCM.40.9.3223-3231.2002.
  10. Rolph HJ. Lennon A, Riggio MP. Saunders WP. MacKenzie D. Coldero Land Bagg J. Molecular identification of microorganisms from endodontic infections. J Clin Microbiol 39: 3282-3289. 2001 https://doi.org/10.1128/JCM.39.9.3282-3289.2001
  11. Conrads, G., J. Seffner. K. Pelz. and R. Mutters. Taxonomic update and clinical significance of species within the genus Peptostreptococcus. Clin Infect Dis 25:S94-97. 1997 https://doi.org/10.1086/516206
  12. Felsenstein, J. PHYLIP (Phylogeny Inference Package) version 3.5c. Distributed by the author. Department of Genetics. University of Washington, Seattle. 1993
  13. Page, R. D. TreeView: an application to display phylogenetic trees on personal computers. Comput Appl Biosci 12:357-358. 1996
  14. Socransky, S. S.. A. D. Haffajee, J. L. Dzink, and J. D. Hillman. Associations. between microbial species in subgingival plaque samples. Oral Microbiol Immunol 3:1-7. 1988 https://doi.org/10.1111/j.1399-302X.1988.tb00596.x
  15. Spratt DA, Weightman AJ and Wade WG. Diversity of oral asaccharolytic Eubacterium species in periodontitis--identification of novel phylotypes representing uncultivated taxa. Oral Microbiol Immunol 14:56-59. 1999 https://doi.org/10.1034/j.1399-302X.1999.140107.x
  16. Nakazawa F, Sato M. Poco SE. Hashimura T. Ikeda T. Kalfas S. Sundqvist G and Hoshino E. Description of Mogibacterium pumilum gen. nov .. sp. nov. and Mogibacterium vescum gen. nov.. sp. nov.. and reclassification of Eubacterium timidum (Holdeman et al. 1980) as Mogibacterium timidum gen. nov., comb. nov. Int J Syst Evol Microbiol 50(2) :679-688, 2000 https://doi.org/10.1099/00207713-50-2-679
  17. Haapasalo M. Kerosuo E and Lounatmaa K. Hydrophobicities of human PMN leukocytes and oral Bacteroides and Porphyromonas spp .. Wolinella recta. and E. yurii with special reference to bacterial surface structures. Scand J Dent Res 98:472-81. 1990
  18. Downes J. Munson MA. Spratt DA. Kononen E, Tarkka E, Jousimies-Somer H and Wade WG. Characterization of Eubacterium-like strains isolated from oral infections. J Med Microbiol 50:947-951. 2001 https://doi.org/10.1099/0022-1317-50-11-947
  19. Cheeseman SL. Hiom SJ. Weightman AJ and Wade WG. Phylogeny of oral asaccarolytic Eubacterium species determined by 16S rRNA sequence comparision and proposal of Eubacterium intirmum sp.nov. and Eubacterium tardum sp.nov. J Sys Bacteriol 46(4): 957-959. 1996 https://doi.org/10.1099/00207713-46-4-957
  20. Hashimura T, Sato M and Hoshino E. Detection of Slackia exigua. Mogibacterium timidum and Eubacterium saphenum from pulpal and periradicular samples using the Polymerase Chain Reaction (PCR) method. Int Endod J 34:463-470. 2001 https://doi.org/10.1046/j.1365-2591.2001.00419.x
  21. Smith AJ. Wade WG. Serum antibody response against oral Eubacterium species. J Periodont Res 34: 175-178, 1999 https://doi.org/10.1111/j.1600-0765.1999.tb02239.x
  22. A.F. Fouad, J. Burleson. The effct of diabetes mellitus on endodontic treatment outcome. J Am Dent Assoc 134:43-51. 2003 https://doi.org/10.14219/jada.archive.2003.0016
  23. Wade WG. Downes J. Dymock D. Hiom SJ. Weightman AJ. Dewhirst FE. Paster BJ. Tzellas N and Coleman B. The family Coriobectetieceee: reclassification of Eubacterium exiguum (Poco et al. 1996) and Peptostreptococcus heliotrinreducens (Lanigan 1976) as Slackia exigua gen. nov.. comb. nov. and Slackia heliotrinireducens gen. nov .. comb. nov .. and Eubacterium len tum (Prevot 1938) as Eggerthella lenta gen. riov.. comb. nov. Int J Syst Bacteriol 49: 595-600. 1999 https://doi.org/10.1099/00207713-49-2-595
  24. Wade WG. Slayne MA and Aldred MJ. Comparison of identification methods for oral asaccharolytic Eubacterium species. J Med Microbiol 33:239-242, 1990 https://doi.org/10.1099/00222615-33-4-239
  25. Wade WG. The role of Eubacterium species in periodontal disease and other oral infections. Microb Ecol Health Dis 9:367-370. 1997 https://doi.org/10.3109/08910609609166480
  26. Weisburg WG. Barns SM, Pelletier DA and Lane DJ. 16S rDNA amplification for phylogenetic study. J Bacteriol 173:697-703, 1991 https://doi.org/10.1128/jb.173.2.697-703.1991

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