Journal of Asia-Pacific Entomology

Korean Society of Applied Entomology (한국응용곤충학회)
권호 표지

Identification of Novel Insecticidal cry1-type Genes from Bacillus thuringiensis Strains Isolated in Korea

  • Park, Jae-Young (School of Agricultural Biotechnology, College of Agriculture & Life Sciences, Seoul National University) ;
  • Li, Ming-Shun (School of Agricultural Biotechnology, College of Agriculture & Life Sciences, Seoul National University) ;
  • Roh, Jong-Yul (School of Agricultural Biotechnology, College of Agriculture & Life Sciences, Seoul National University) ;
  • Shim, Hee-Jin (School of Agricultural Biotechnology, College of Agriculture & Life Sciences, Seoul National University) ;
  • Boo, Kyung-Saeng (School of Agricultural Biotechnology, College of Agriculture & Life Sciences, Seoul National University) ;
  • Je, Yeon-Ho (School of Agricultural Biotechnology, College of Agriculture & Life Sciences, Seoul National University)
  • Published : 2003.11.01
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Abstract

To identify new cry1-type crystal protein genes, 52 Bacillus thuringiensis isolates which produced bipyramidal shaped crystal inclusions were selected. PCR-RFLP analysis of 52 Bt isolates revealed 6 distinct patterns, and 6 standard isolates were selected from each RFLP group. Through cloning and restriction enzyme analysis of the PCR- amplified fragments showing unique RFLP pattern, 6 novel cry1-type genes were identified and named cry1-201, cry1-202, cry1-203, cry1-205, cry1-206 and cry1-207, respectively. In the nucleotide sequence analysis of these novel crystal genes, cry1-201, cry1-202 and cry1-203 showed 93.7%, 94.5% and 66.7% of maximum similarities with cry1Ac gene, respectively. Also, cry1-205 and cry1-207 showed 99.5% and 99.4% maximum similarities with cry1Be gene, and cry1-206 showed 97.5% maximum similarity with cry1Ab gene.

Keywords

References

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