Journal of the East Asian Society of Dietary Life (동아시아식생활학회지)

The East Asian Society of Dietary Life (동아시아식생활학회)
권호 표지

Antimicrobial Activities of Berry Extract of Domestic Plants on 4 Kind of Pathogenic Microorganism

4종의 식품 부패 미생물에 대한 국내산 자생 식물 열매 추출물의 항균성 탐색

  • 권민경 (성신여자대학교 식품영양학과) ;
  • 이해은 (성신여자대학교 식품영양학과) ;
  • 박주연 (성신여자대학교 식품영양학과) ;
  • 한영숙 (성신여자대학교 식품영양학과)
  • Published : 2003.10.01
Download PDF
⟨ Previous Next ⟩

Abstract

To select new useful domestic plants with antimicrobial activities, thirty five samples of berry plant were distributed from Plant Diversity Research Center in Korea Research Institute of Bioscience and Biotechnology. Their extracts with methanol were tested against Escherichia coli, Salmonella typhimurium, Staphylococcus aureus, Listeria monocytogenes by paper disc method. The methanol extracts from Viburnum dilatatum, Viburnum erosum, Aralia continentalis, Camellia japonica, Acer trumcatam, Arasaema takesimense and Rhysocarpus intermedius were effective against S. aureus and the results were as follows: Viburnum erosum 6.5 mm, Viburnum dilatatum and Aralia continentalis 7.0 mm, Rhysocarpus intermedius 8.0 mm, Acer trumcatum 9.0 mm, Camellia japonica and Arisaema takesimense 9.5 mm. And only the methanol extract from Camellia japonica was effective against L. monocytogenes, 7.0 mm. Antimicrobial activities of E. coli and S. typhymurium were not detected. The minimum inhibitory concentration(MIC) of berry of Camellia japonica was examined as 1,250 $\mu\textrm{g}$/mL against S. aureus and as 1,250∼2,500 $\mu\textrm{g}$/mL against L. monocytogenes.

4종류의 식중독 세균 E. coli, S. typhimurium, S. aureus, L. monocytogenes 에 대하여 35 종의 국내산 자생 식물 열매 추출물에 의한 항균작용을 조사하였다. 1 mg/disc 농도의 추출물을 6.0 mm paper disc 에 흡수시켰으며 disc 주위의 clear zone의 직경 (mm)을 생육저해환으로서 비교하였다. S. aureus에서는 덜꿩나무가 6.5 mm, 가막살나무와 독활이 각각 7.0 mm, 신나무가 8.0 mm 동백나무 와 만주고로쇠가 각각 9.5 mm의 clear zone을 보이며 우수한 항균성을 가진 것으로 검색되었고 L. monocytogenes에서는 동백나무 열매 추출물만 7.0 mm의 clear zone이 나타났으며 E. coli 와 S. typhimurium에서는 모든 추출물이 항균성을 나타내지 않았다. 또한 S. aureus 와 L. monocytogenes에 항균성을 보인 동백나무 열매 MeOH 추출물의 최소 저해 농도를 측정한 결과 S. aureus에 대하여 1,250 $\mu\textrm{g}$/mL, L. monocytogenes에 대하여 1,250∼2,500 $\mu\textrm{g}$/mL로 나타났다. 이상의 본 연구 결과는 35종의 국내산 자생 식물 열매의 항균 효과를 검토하고 동백나무 열매의 최소 저해 농도를 측정하여 향후 천연 항균 소재 개발에 필요한 기초 자료를 제시하였다.

Keywords

References

  1. 익생양술 권혁세
  2. 현대식품위생학 송형익;채기수;김영만;손규목;이웅수
  3. 자세히 쓴 식품위생학 장동석;신동화;정덕화;김창민;이인선
  4. 산업보건 v.136 우리나라의 식중독 발생현황과 대책 허영주
  5. Korean J Food Sci Technol v.32 no.6 Isolation and identification of active antimicrobial substance againse Listeria monocytogenes from Ruta graveolens Linne Ahn,Y.S.;Shin,D.H.;Baek,N.I.
  6. J Food Prot v.62 Survival of enterohemorrhagic Escherichia coli O157:H7 in bovinefeces applied to lettuce and effectiveness of chlorinated water as disinfectant Beuchat,L.R.
  7. J Food Prot v.62 Reduction of Escherichia coli O157:H7 and Salmonella typhimurium on beef carcass surfaces using acidified sodium chlorite Castillo,A.;Lucia,L.M.;Kemp,G.K.;Acuff,G.R.
  8. The editorial staff of the journal of San Yatsen Univ A taxonomy of the genus Camellia Chang,H.T.
  9. Kor J Appl Microbiol Biotechnol v.25 no.3 Antimicrobial activities of pine needle (Pinus densiflora Seib et Zucc.) extract Choi,M.Y.;Choi,E.J.;Lee,E.;Rhim,T.J.;Cha,B.C.;Park,H.J.
  10. Korean J Soc Food Sci v.14 no.5 Screeing of antimicrobial activity of the plantain(Plantago asiatica L.)extract Jeon,Y.O.;Kim,K.H.;Kim,S.I.;Han,Y.S.
  11. J Food Prot v.61 Growth and survival of Listeria monocytogenes in vacuum-packaged ground beef inoculated with Lactobacillus alimentarius Flora Cam L-2 Juven,B.J.;Barefoot,S.F.;Pierson,M.D.;Maccaskill,L.H.;Smith,B.
  12. J Food Prot v.62 Effect of diacetyl controlling Escherichia coli O157:H7 and Salmonella typhimurium in the presence of starter culture in a laboratory medium and during meat fermentation Kang,D.H.;Fung,D.Y.C.
  13. J Korean Soc Food Sci Nutr v.27 no.2 Proximate, saponin and amino acid compositions in Camellia(Camellia japonica L.) seeds and defatted camellia seeds Kang,S.K.;Kim,Y.D.;Choi,O.J.
  14. J Food Prot v.64 no.8 Antibacterial activity in extracts of Camellia japonica L. petals and its application to a model food system Kim,K.Y.;Davidson,P.M.;Chung,H.J.
  15. J Food Sci v.46 Effects of potassium sorbate alone and in combination with sodium chloride on the growth of Salmonella typhimurium 7136 Larocco,K.A.;Martin,S.E.
  16. Agricultural Chemistry and Biotechnology v.38 no.3 Antibacterial activities of extracts from Chrysanthemum boreale M. Nam,S.H.;Yang,M.S.
  17. Korean J Food Sci Technol v.30 no.4 Antimicrobial activities of natural medicinal herbs on the food spoilage or foodbone disease microorganisms Oh,D.H.;Ham,S.S.;Park,B.K.;Ahn,C.;Yu,J.Y.
  18. Korea J Fd Hyg Safety v.16 no.4 Monitoring and trands analysis of food poisoning outbreaks occurred in recent years in Korea Park,H.O.;Kim,C.M.;Woo,G.J.;Park,S.H.;Lee,D.H.;Chang,E.J.;Park,K.H.
  19. J Food Sci v.62 Effectiveness of sanitizing agents in inactivating Escherichia coli in golden delicious apples Sapers,G.M.;Miler,R.L.;Mattazzo,A.M.
  20. Food Microbiol v.16 A bacteriocin from Carnobacterium piscicola for the control of Listeria monocytogenes in vacuum packaged meat Schobitz,R.;Zaror,T.;Leon,O.;Costa,M.
  21. Korean J Nutrition v.28 no.11 Studies on the antimicrobial effect of wasabi extracts Seo,K.L.;Kim,D.Y.;Yang,S.I.
  22. J Food Sci v.56 Growth temperature and action of lysozyme on Listeria monocytogenes Smith,J.L.;Marmer,B.S.
  23. Food Microbiol v.13 Evaluation of lactic acid and monolaurin to control Listeria monocytogenes on stacchino cheese Stecchini,M.L.;Luch,R.D.;Bortolussi,G.;Deltorre,M.
  24. J Food Prot v.62 Behavior of enterohemorrhagic Escherichia coli O157:H7 on alfalfa sprouts during the sprouting process as influenced by treatments with various chemicals Taormina,P.J.;Beuchat,L.R.
  25. J Food prot v.62 Inactivation of Escherichia coli O157:H7 and and Listeria monocytogenes on plastic kitchen cutting boards by electrolyzed oxidizing water Venkitanarayanan,K.S.;Ezeike,G.O.I.;Hung,Y.C.;Doyle,M.P.
  26. Agricultural Chemistry and Biotechnology v.38 no.6 Screening of Domestic plants with antibacterial activity Yang,M.S.;Ha,Y.L.;Nam,S.H.;Choi,S.U.;Jang,D.S.
  27. J Food Sci v.54 Inactivation and injury of Listeria monocytogenes in a minimal medium as affected by benzoic acid and incubation temperature Yousef,A.E.;EL-Shenawy,M.A.;Marth,E.H.
  28. 新食産業 v.25 抗菌性天然添加物開發の現狀と使用上の問題鮎 芝崎勳