Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
권호 표지

Embryo Transfer with Sex-Determined Hanwoo Embryos Produced by In-vitro Fertilization

성감별된 한우 체외수정란의 수정란 이식

  • 김용준 (전북대학교 수의과대학) ;
  • 이창민 (전북대학교 수의과대학) ;
  • 정구남 (전라북도 축산진흥연구소 종축시험소) ;
  • 이해리 (전북대학교 수의과대학) ;
  • 조성우 (전라북도 축산진흥연구소 종축시험소) ;
  • 김용수 (전라북도 축산진흥연구소 종축시험소) ;
  • 신동수 (전라북도 축산진흥연구소 종축시험소) ;
  • 홍유미 (전북대학교 수의과대학) ;
  • 유일정 (전북대학교 수의과대학)
  • Published : 2003.08.01
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Abstract

In-vitro fertilized Hanwoo embryos were biopsied for sex determination by PCR. Biopsied embryos were incubated for 1∼2 hours for the recovery. Those sexed Hanwoo embryos were transferred to 49 Hanwoo and 16 Holstein recipients from February 2000 to February 2001. Of 65 recipients, 14 cows(12 Hanwoo and 2 Holstein) delivered the same offspring as sex-determined by PCR, therefore the conception rate was 21.5%. 1. Total 65 embryos(male 35, female 30) were transferred to recipients, and 14 calves (male 6, female 8) were delivered. In comparison between sex by PCR method and sex of calves born after embryo transfer, the accuracy of sex determination was 100.0%. 2. The conception rate after transfer with biopsied embryo between Hanwoo and Holstein was 24.5% and 12.5% 3. The conception rate after transfer with biopsied embryo between fresh and frozen-thawed embryos was 23.5% and 14.3%. 4. The conception rate according to the season when embryo was transferred was 11.8, 29.4, 23.5 and 20.0% for spring, summer, autumn and winter, respectively. 5. The conception rate according to embryo quality after biopsy was 41.7, 30.0 and 0.0% for excellent, good and fair quality. 6. The conception rate according to thickness of uterine horn was 71.4, 18.9, 11.8 and 0.0% for 0, +, ++ and +++ thickness. 7. The conception rate according to the site in the uterine hem where embryo was put was 30.0, 20.0 and 10.0% for cranial, mid, and caudal part of uterine horn. 8. The conception rate according to the quality of corpus luteum ipsilateral to the uterine horn where embryos was transferred was 41.2, 14.3 and 15.4% for excellent, good and fair quality. 9. The conception rate according to the time required for embryo transfer was 18.2, 30.0, 30.0, 0.0 and 25.0% for 10, 15, 20, 25 and 30 minutes. 10. The conception rate according to parity of recipients was 26.5, 19.1, 14.3 and 0.0% for the primiparous, the 2nd parous, the 3rd parous and the 4th parous recipients. These results indicated that fresh embryos are more demanded than frozen-thawed embryos for good conception rate in embryo transfer with biopsied-sexed embryo. Also, it was indicated that we should consider embryo-recovering condition, recipient's uterine thickness, transfer site in uterine horn, quality of corpus luteum, time required for transfer and parity of recipient to achieve good conception rate in ET with biopsied-sexed embryos.

체외수정된 한우 수정란을 PCR 기법에 의한 성감별을 위해 생검하여 회복시까지 1∼2시간 동안 배양하였다. 이 성감별된 한우 수정란을 2000년 2월부터 2001년 2월까지 한우 49두와 젖소 16두에 이식하였다. 수정란이 이식된 65두의 수란우중 14두(한두 12두, 젖소 2두)가 성감별 결과와 동일한 성의 산자를 생산하였고, 전체 수태율은 21.5%를 나타내었다. 1. 총 65두의 수란우중 웅성 수정란은 35두에 자성 수정란은 30두에 이식되었고 이중 분만된 산자 14두중 웅성은 6두, 자성은 8두였으며 PCR에 의한 성감별 정확도는 100.0%를 나타내었다. 2. 성감별된 한우 수정란은 한우와 젖소에 이식한 후 수태율은 각각 24.5, 12.5%를 나타내었다. 3. 성감별된 한우의 신선수정란과 동결-융해된 수정란의 이식후 수태율은 각각 23.5, 14.3%를 나타내었다. 4. 성감별된 한우 수정란의 이식 시기에 따른 수태율은 봄, 여름, 가을, 겨울에 각각 11.8, 29.4, 23.4, 20.0%를 나타내었다 5. 성감별된 한우 수정란의 상태에 따른 이식후 수태율은 excellent, good, fair군에서 각각 41.7, 30.0, 0.0%를 나타내었다. 6. 성감별된 한우 수정란의 이식시 수란우의 자궁각 두께에 따른 이식후 수태율은 0, +, ++, +++군에서 각각 71.4, 18.9, 11.8, 0.0%를 나타내었다. 7. 성감별된 한우 수정란이 주입된 수란우 자궁각 부위에 따른 이식후 수태율은 자궁각 선단부, 중심부, 미부에서 각각 30.0, 20.0, 10.0%를 나타내었다. 8. 성감별된 한우 수정란의 이식시 수란우의 황체의 상태에 따른 이식후 수태율은 excellent, good, poor군에서 각각 41.2, 14.3, 15.4%를 나타내었다. 9. 성감별된 한우 수정란을 straw에 장착후 이식시까지 10, 15, 20, 25, 30분 경과에 따른 이식후 수태율은 18.2, 30.0, 30.0, 0.0, 25.0%를 나타내었다. 10. 성감별된 한우 수정란의 이식시 수란우의 산차에 따른 이식후 수태율은 1산차, 2산차, 3산차, 4산차에서 각각 26.5, 19.1, 14.3, 0.0%를 나타내었다. 이상의 결과에서 성감별된 수정란의 이식시 양호한 수태율을 얻기 위해서는, 신선수정란이 동결수정란 보다 유리하다는 점과 생검후 수정란의 회복상태, 수란우의 자궁각 두께, 수정란의 수란우 자궁각 주입부위, 수란우의 황체 상태, 이식시까지 소요시간, 수란우의 산차를 고려해야 한다는 것을 알 수 있었다.

Keywords

References

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