Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
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Studies on Preservation of Germ Cells in Hanwoo I. Effects of Frozen Thawed Viability and Characteristics in Bovine Semen

한우의 생식세포 보존에 관한 연구 I. 한우 정액의 일반성상 및 동결후 생존성에 미치는 영향

  • 이명식 (농촌진흥청 축산기술연구소) ;
  • 박정준 (농촌진흥청 축산기술연구소) ;
  • 전기준 (농촌진흥청 축산기술연구소) ;
  • 정영훈 (농촌진흥청 축산기술연구소) ;
  • 우제석 (농촌진흥청 축산기술연구소) ;
  • 박수봉 (농촌진흥청 축산기술연구소) ;
  • 임석기 (농촌진흥청 축산기술연구소) ;
  • 연성흠 (농촌진흥청 축산기술연구소) ;
  • 손동수 (농촌진흥청 축산기술연구소)
  • Published : 2003.08.01
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Abstract

This study was carried out to cryopreserve and to investigate characteristics of semen in Hanwoo. Semen was obtained from bulls selected by Daekwanryeong Branch station. Semen was collected each morning of the experiment, placed in water jacketed tubes at 37$^{\circ}C$, and trans-ported to the research laboratory within 10 minutes. Semen was extended with Egg yolk-glycerol extender to contain 50${\times}$10$^{6}$ sperm/ml. Semen was cooled over a 6h period in water jacketed tubes from about 25 to 5$^{\circ}C$, Egg yolk-glycerol extender was added in one step at 5$^{\circ}C$. Semen was aspirated into 0.5ml straws, which were sealed with powder. Egg yolk-glycerol extender, which is used in Hanwoo sperm frozen and stored, semen from 13 Hanwoo bulls collected, the postthawed percentages of motile sperm were 65.7%. In semen characteristics of Hanwoo bulls, number of bulls volume are 5.7 ml and total cell count are 975${\times}$10$^{6}$ m1 ejaculate.

한우의 유전자원을 보존하기 위하여 정액동결 시험을 수행하였으며 고급육계통은 육질에 대한 육종가 상위 10% 이내의 자손에서 선발하였고 다 유계통은 어미의 이유시 체중에 대한 육종가 상위 10%이내의 자손에서 선발하였으며 2개 계통에서 종모우 총 13두를 선발하여 공시하였다. 정액채취는 인공질법으로 실시하였고 의빈대에 수소를 계류하고 채정대상우를 승가시켰으며, 3회 가승가후에 채정하였다. 채정후 10분 이내에 실험실로 옮겨와 검사항목을 조사하고 37$^{\circ}C$에서 1차 희석을 하고 5$^{\circ}C$까지 하강한 후 2차 희석을 하였으며, 액체 질소 5cm 위에서 5분간 평형후 침지하여 동결하였다. 이들 종모우 정액의 일반적 성상에 있어서 채정된 정액량은 1차와 2차를 합하였을 때 평균 채정량이 5.7 ml였고 정자농도는 975${\times}$$10^{6}$개였으며 정액의 색상은 유백색이었고 pH는 6.8이었다. 채취시 생존성은 90.2% 그리고 동결 융해후에는 65.7 %가 생존하였으며 총 6,870스트로우의 동결 정액을 생산하여 보존하였다.

Keywords

References

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