Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Cytogenetic Analyses of Angelica Plants Using Feulgen Staining and Multicolor Fluorescence in Situ Hybridization

Multicolor FISH와 Feulgen 염색법을 이용한 Angelica속 식물의 세포유전학적 분석

  • Koo, Dal-Hoe (Department of Biology, Chungnam National University) ;
  • Kim, Soo-Young (Department of Biology, Chungnam National University) ;
  • Bang, Kyong-Hwan (Industrial Crop Division, National Crop Experimental Station, RDA) ;
  • Seong, Nak-Sul (Industrial Crop Division, National Crop Experimental Station, RDA) ;
  • Bang, Jae-Wook (Department of Biology, Chungnam National University)
  • Published : 2003.06.01
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Abstract

Karyotype analysis and chromosomal localization of 5S and 45S rDNAs using multi-color fluorescence in situ hybridization (McFISH) technique were carried out in two Angelica species. The numbers of diploid chromosomes were the same in two same in two species as 2n=22, however the lengths of chromosomes were varied from 4.25 to 6.50 ${\mu}{\textrm}{m}$ in A gigas and 4.95 to 8.50 ${\mu}{\textrm}{m}$ in A acutiloba. The chromosomes of A. gigas were composed of five metacentric and six submetacentric pairs, while those of A. acutiloba were six metacentic, one submetacentric and four subtelocentric paris. In FISH experiments, the numbers and size of 45S rDNA signals were varied between two species, however dach signal of the 5S rDNA was observed in two species.

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