Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Micropropagation via Axillary Bud Induction of Eucalyptus pellita

액아유도에 의한 Eucalyptus pellita의 기내번식

  • Moon, Heung-Kyu (Division of Biotechnology, Forestry Research Institute (KFRI)) ;
  • Kim, Ji-Ah (Division of Biotechnology, Forestry Research Institute (KFRI)) ;
  • Lee, Hyun-Shin (Kantor Pusat, JI. Korindo) ;
  • Kang, Ho-Duck (Depart. of Forest Resources, Dongguk University)
  • 문홍규 (임업연구원 생물공학과) ;
  • 김지아 (임업연구원 생물공학과) ;
  • 이현신 (인도네시아 코린도 그룹 KTH사) ;
  • 강호덕 (동국대학교 산림지원학과)
  • Published : 2003.09.01
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Abstract

In order to develop an efficient micropropagation protocal for Eucalyptus pellita, on in vitro culture system has been was established by inducing axillary buds from greenhouse stock materials. Among 6different media tested, DKW medium was the best ot induce bast induce both shoot proliferation and growth. Average number of proliferated shoots of 403per explant was obtained at the concentration of 0.1mg/LBA. Most of the stem materials excreted phenolic compounds at the proximal part of the explant and caused darking of the media. Therefore, it was necessary to transfer frequently to a fresh medium and/or to add activated charcoal at the concentration of 0.02％(w/v). Generally on vitro roots were formed easily on 1/2DKW medium with NAA treatment. All the explants rooted at the medium containing 0.2mg/L NAA and displayed vigorous root growth in vitro culture conditions. After transferred to an artificial soil mixture (peatmoss: vermiculrite: perlite, 1:1:1, v/v/v) in the greenhouse, most rooted plantlets survived well without any morphological abnormalities. The results show that the species can be micropropagated effectively by the application of axillary bud culture system.

Keywords

References

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