Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
권호 표지
DOI QR코드

DOI QR Code

High-frequency Plant Regeneration from Mature Seed-derived Callus Cultures of Orchardgrass

오차드그래스 성숙종자로부터 캘러스 유도 및 고효율 식물체 재분화

  • Lee, Sang-Hoon (Division of Applied Life Science, Gyeongsang National University) ;
  • Lee, Dong-Gi (Division of Applied Life Science, Gyeongsang National University) ;
  • Kim, Jin-Soo (Division of Applied Life Science, Gyeongsang National University) ;
  • Lee, Byung-Hyun (Institute of Agriculture and Life Science)
  • 이상훈 (경상대학교 응용생명과학부) ;
  • 이동기 (경상대학교 응용생명과학부) ;
  • 김진수 (경상대학교 응용생명과학부) ;
  • 이병현 (농업생명과학연구원)
  • Published : 2003.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

In an effort to optimize tissue culture conditions for genetic transformation of orchardgrass (Dactylis glomerata L.), an efficient and high-frequency plant regeneration system from seed-derived calli was established. Embryogenic calli induced on MS medium containing 3mg/L 2,4-D and 0.1mg/L BA had significantly improved regeneration ability. Plant regeneration rate was 92％ when embryogenic calli were cultured on N6 medium supplemented with 1mg/L 2,4-D and 3mg/L BA. Among three kinds of medium, MS and N6 medium were optimal for embryogenic callus induction and plant regeneration, respectively. Ho difference in callus induction frequency was observed among four cultivars of orchardgrass, however, "Roughrider" cultivar showed higher regenerability with the frequency of 61％. Addition of maltose to the regeneration medium as a carbon source dramatically increased regeneration frequency up to 69％. A short tissue culture period and high-frequency regeneration system would be beneficial for molecular breeding of orchardgrass through genetic transformation.

Keywords

References

  1. Altpeter F, Xu J (2000) Rapid production of transgenic turfgrass (Festuca rubra L.) plants. J Plant Physiol 157: 441-448 https://doi.org/10.1016/S0176-1617(00)80029-2
  2. Bai Y, Qu R (2000) An evaluation on callus induction and plant regeneration of 25 turf-type tall fescue (Festuca arundinacea Schreb.) cultivars. Grass Forage Sci 55: 326-330 https://doi.org/10.1046/j.1365-2494.2000.00235.x
  3. Chaudhury A, Rongda Q (2000) Somatic embryogenesis and plant regeneration of turf-type Bermuda grass: effect of 6-benzy-ladenine in callus induction medium. Plant Cell Tiss Org Cult 60: 113-120 https://doi.org/10.1023/A:1006456005961
  4. Cho MJ, Jiang W, Laumaux PG (1998) Transformation of recalcitrant barley cultivars through improvement of regenerability and decreased albinism. Plant Sci 138: 229-244 https://doi.org/10.1016/S0168-9452(98)00162-9
  5. Cho MJ, Choi HW, Lemaux PG (2001) Transformed orchardgass (Dactylisglomerata L.) plants produced from regenerative tissues derived from mature seeds. Plant Cell Rep 20: 318-324 https://doi.org/10.1007/s002990100330
  6. Chu CC, Wang CS, Sun CC, Hsu C, Yin KC, Chu CY, Bi FY (1975) Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources. Scienta Sinic 18: 659-668
  7. Conger BV, Hanning GE, Gray DJ, McDaniel JK (1983) Direct embryogenesis from methophyll cells of orchardgrass. Science 221: 850-851 https://doi.org/10.1126/science.221.4613.850
  8. Conger BV, Hovanesian JC, Trigano R, Gray DJ (1989) Somatic embryo ontogeny insuspension cultures of orchardgrass. Crop Sci 29: 448-452 https://doi.org/10.2135/cropsci1989.0011183X002900020042x
  9. Denchev PD, Songstad DD, McDaniel JK, Conger BV (1997) Transgenic orchardgrass (Dactylisglomerata L.) plants by direct embryogenesis from microprojecticle bombarded leaf cells. Plant Cell Rep 16: 813-819 https://doi.org/10.1007/s002990050326
  10. Forster JW, Spangenberg G (1999) Forage and turf grass biotechnology: principles, methods and prospects. In: Setlow JK (eds), Genetic engineering: principles and methods, Vol 21, Kluwer Academic Publishers, NewYork, pp 191-237
  11. Griffin JD, Dibble MS (1995) High frequency plant regeneration from seed-derived callus cultures of Kentucky bluegrass (Paa pratensis L.). Plant Cell Rep 14: 721-724
  12. Hom ME, Shillito RD, Conger BV, Harms CT (1988) Transgenic plants of orchardgrass (Dactylis glomerata L.) from protoplasts. Plant Cell Rep 7: 469-472 https://doi.org/10.1007/BF00272733
  13. McKersie BD (1997) Improving forage production systems using biotechnolgy. In: McKersie BD and Brown DCW (eds), Biotechnology in Agriculture Series, No. 17, CAB International, Wall-ingford, pp 3-21
  14. Miller DA (1984) Forage crops. McGraw-Hili, NewYork, pp 396-409
  15. Murashige T, Skoog F (1962) A revise medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473-497 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  16. Rim YW, Kim KY, Choi KJ, Sung BR, Shin JS (2000) Callus induction from seeds of Italian ryegrass and plant regeneration. J Korean Grassland Sci 20: 25-30
  17. Schenk RU, Hildebrand AC (1972) Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures. Can J Bot 50: 199-204 https://doi.org/10.1139/b72-026
  18. Spangenberg G, Wang ZY, Potrykus (1998) Biotechnology in forage and turf grass improvement. In: Frankel et al (eds), Monographs on theoratical and applied genetics, Vol. 23, Springer Verlag, Heidelberg, pp 192-210
  19. Van der Valk P, Ruis F, Tettelaar-Schrier AM, van der Velde CM (1995) Optimizing plant regeneration from seed-derived callus cultures of Kentucky bluegrass, the effect of benzyladenine. Plant Cell Tiss Org Cult 40: 101-103 https://doi.org/10.1007/BF00041125
  20. Van Santen E, Sieper DA (1996) Orchardgrass. In: Moser LE et al (eds), Cool-season forage grasses. Vol 34, ASA, CSSA, and SSSA, Madison WI. pp 503-534
  21. Van Wijk AJP, Boonman JG, Rumball W (1993) Achievements and prospectives in the breeding of forage grasses and legumes. In: Baker MJ (eds), Grasslands for our world, SIR, Wellington, pp 116-120
  22. Vasil V, Vasil lK (1984) Induction and maintanance of embryogenic callus cultures of Gramineae. In: VasillK (eds), Cell culture and somatic cell genetics of plants, Vol 1, Academic Press, Orlando, pp36-42
  23. Wang ZY, Nagel J, Potrykus I, Spangenberg G (1993) Plants from cell suspension-derived protoplasts in Lollium species. Plant Sci 94: 179-193 https://doi.org/10.1016/0168-9452(93)90019-V
  24. Ye X, Wang ZY, Wu X, Potrykus I, Spangenberg G (1997) Transgenic Italian ryegrass (Lolium multiflorum) plants from microprojectile bombardment of embryogenic suspension cells. Plant Cell Rep 16: 379-384 https://doi.org/10.1007/BF01146777
  25. Zhong H, Sticklen MB (199l) Plant regeneration via somatic embryogenesis in creeping bentgrass (Agrostis palustris Huds). Plant CellRep 10: 453-456

Cited by

  1. Factors Affecting Callus Induction and Plant Regeneration from Mature Seed of Zoysiagrass (Zoysia japonica Steud.) vol.24, pp.1, 2004, https://doi.org/10.5333/KGFS.2004.24.1.029
  2. Development of High Quality Forage Grass by Down-regulating Lignin Biosynthetic Gene vol.26, pp.1, 2006, https://doi.org/10.5333/KGFS.2006.26.1.001
  3. Efficient Callus Culture and Plant Regeneration from Mature Seed of Tall Fescue (Festuca arundinacea Schreb.) vol.32, pp.3, 2005, https://doi.org/10.5010/JPB.2005.32.3.187
  4. In Vitro Plant Regeneration of Siberian Wildrye Grass from Mature Seed-derived Callus vol.31, pp.3, 2011, https://doi.org/10.5333/KGFS.2011.31.3.217
  5. In vitro Technique for Selection of Radiation Induced Mutants of Tall Fescue vol.19, pp.1, 2013, https://doi.org/10.11109/JAES.2013.19.1.063
  6. Plant Regeneration from Mature Seed-Derived Callus in Bermudagrass (Cynodon dactylon) vol.31, pp.3, 2011, https://doi.org/10.5333/KGFS.2011.31.3.223
  7. Effect of Plant Growth Regulators on Callus Induction and Plant Regeneration from Seed Culture of Reed vol.31, pp.3, 2011, https://doi.org/10.5333/KGFS.2011.31.3.229
  8. Plant Regeneration From Mature Seed of Domestic Italian Ryegrass Cultivar vol.31, pp.3, 2011, https://doi.org/10.5333/KGFS.2011.31.3.235