Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
권호 표지
DOI QR코드

DOI QR Code

Mass Propagation of Liriodendron tulipifera L. via Somatic Embryogenesis

체세포 배발생을 통한 백합나무 [Liriodendron tulipifera L.]의 대량증식

  • Lee, Jae-Soon (Biotechnology Division, Korea Forest Research Institute) ;
  • Moon, Heung-Kyu (Biotechnology Division, Korea Forest Research Institute) ;
  • Kim, Yong-Wook (Biotechnology Division, Korea Forest Research Institute)
  • 이재순 (임업연구원 생물공학과) ;
  • 문흥규 (임업연구원 생물공학과) ;
  • 김용욱 (임업연구원 생물공학과)
  • Published : 2003.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

Mass propagation of tulip tree (Liriodendron tulipifera L) via somatic embryogenesis was successfully achieved with immature samaras collected from adult trees. Embryogenic tissues were induced by culturing them samaras on 1/2 LM medium (Litvay's) containing 2,4-D and BA. Somatic embryos developed from the embryogenic tissues and germinated to normal plants (emblings) upon transfer onto the same medium containing either AgNO$_3$ or activated charcoal. So far, several factors appeared to influence both the induction of embryogenic tissues and germination of the embryos into plants. These include the collection time of samaras for the induction of embryogenic tissue, sucrose level in the culture medium, the level of both AgNO$_3$ and activated charcoal, and plating density of somatic embryos on germination medium for maturation and germination of somatic embryos into plantlets.

20년 이상된 백합나무의 성숙목에서 채취한 미숙구과의 배를 배양하여 체세포배를 유도하고 식물체를 생산하는 대량증식의 기초를 확립하였다. 미숙종자를 1/2LM (Litvay)배지에 식물생장조절물질인 2,4-D와 BA를 첨가한 반고체배지에 치상하여 배발생 조직을 유도하였다. 배발생 조직을 배발생 조직 유도와 같은 조건의 배지에서 생장조절물질을 제거하고 AgNO$_3$또는 활성탄을 첨가한 배지에 이식하였을 때 체세포배가 발달하고 정상적인 식물체로 발아되었다. 배발생 조직의 유도와 체세포배의 발아에서 식물체로 발달하는 데 영향을 끼치는 몇 가지 요인이 밝혀졌다. 여기에는 배발생 조직의 유도를 위한 미숙종자의 채취시기, 배지의 sucrose농도,AgNO$_3$와 활성탄의 농도 및 체세포배에서 식물체로 발달하기 위한 배의 성숙과 발아를 위하여 발아 배지에 체세포배의 치상밀도 등이 포함된다.

Keywords

References

  1. Al-Khayri JM, AI-Bahrany AM (2001) Silver nitrate and 2-isopenty-ladenine promote somatic embryogenesis in date palm (Phoenix dactylifera L.). Sci Hort 89: 291-298 https://doi.org/10.1016/S0304-4238(00)00244-2
  2. Aronen T, Nikkanen T, Hggman H (1998) Compatibility of different pollination techniques with microprojectile bombardment of Norway spruce and Scots pine pollen. Can J For Res 28: 79-86 https://doi.org/10.1139/cjfr-28-1-79
  3. Durzan DJ, Gupta PK (1987) Somatic embryogenesis and polyem-bryogenesis in Douglas fir cell suspension cultures. Plant Sci 52: 229-235 https://doi.org/10.1016/0168-9452(87)90056-2
  4. Fuentes SRL, Calheiros MBP, Manetti-Filho J, Vieira LGE (2000) The effects of silver nitrate and different carbohydrate sources on somatic embryogenesis in Coffea canephora. Plant Cell Tiss Org Cult 60: 5-13 https://doi.org/10.1023/A:1006474324652
  5. Kavanach K, Chrleton TJ (1990) Seed production and dispersal pattems in populations of Uriodendron tulipifera at the northern edge of its range in southern Ontario, Canada. Can J For Res 20: 1461-1470 https://doi.org/10.1139/x90-193
  6. Lee KS, Lee JC, Soh WY (2002) High frequency plant regeneration from Aralia cordata somatic embryos. Plant Cell Tiss Org Cult 68: 241-246 https://doi.org/10.1023/A:1013989707725
  7. Utvay JD, Verma DC, Johnson MA (1985) Influence of a loblolly pine (Pinus taeda L.) culture medium and its components on growth and somatic embryogenesis of the wild carrot (Daucus carota L.). Plant Cell Rep 4: 325-328 https://doi.org/10.1007/BF00269890
  8. Lu CY, Thorpe TA (1987) Somatic embryogenesis and plantlet regeneration in cultured immature embryos of Picea glauca. J Plant Physiol 128: 297-302 https://doi.org/10.1016/S0176-1617(87)80244-4
  9. Merkle SA, Sommer HE (1986) Somatic embryogenesis in tissue cultures of Liriodendron tulipifera. Can J For Res16: 420-422 https://doi.org/10.1139/x86-077
  10. Moon HK, Hong YP, Kim YW, Lee JS (2001) Genotype effect on somatic embryogenesis and plant regeneration of 15 Aralia elata. J Kor For Soc 28: 129-134
  11. Moon HK, Oh KE, Son SH (1999) Factors influencing somatic embryo induction and plant regeneration in Aralia elata Seem. Kor J Plant Tiss Cult 26: 275-280
  12. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue culture. Physiol Plant 15: 473-497 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  13. Nishiwaki M, Fujino K, Koda Y, Masuda K, Kikuta Y (2000) Somatic embryogenesis induced by the simple application of abscisic acid to carrot (Daucus carota L.) seedling in culture. Plante 211: 756-759 https://doi.org/10.1007/s004250000387
  14. Nhut DT, Le BV, Fukai S, Tanaka M, Van KTI (2001) Effects of acti-vated charcoal, explant size, explant position and sucrose concentration on plant and shoot regeneration of Lilium longiflorum uia young stem culture. Plant Grow Reg 33: 59-65 https://doi.org/10.1023/A:1010701024963
  15. Park HB, Choi EG, Park BM (1993) Somatic embryogenesis and plantre generation from immature ovule of Vitis flexuosa Thunberg. Kor J Plant Tiss Cult 20: 109-112
  16. Ryu KO (2003) Adaptability of Liriodendron tulipifera Linne in Korea and techniques for producing its seedlings. PhD thesis, Chungbuk National University, Cheongju, Korea
  17. Sommer HE, Brown CL (1980) Embryogenesis in tissue culture of sweetgum. Forest Sci 26: 257-260
  18. Sotak, RJ, Sommer HE, Merkle SA (1991) Relation of the develop-mentalstage of zygotic embryosof yellow-poplar to theirsomatic embryogenic potential. Plant Cell Rep 10: 175-178
  19. Walter C, Grace IJ, Wagner A, White DWR, Walden AR, Donaldson SS, Hinton H, Gardner RC, Smith DR (1998) Stable transformation and regeneration of transgenic plantsof Pinus radiata D. Don. Plant Cell Rep 17: 460-468 https://doi.org/10.1007/s002990050426
  20. Webb DT, Webster F, Flinn BS, Roberts DR, Ellis DO(1989) Factors influencing the induction of embryogenic and caulogenic callus from embryo of Picea glaucaand P. engelmanii. Can J For Res 19: 1303-1308 https://doi.org/10.1139/x89-200
  21. Wetzstein FH, Ault JR, Merkle SA (1989) Further characterization of somatic embryogenes is and plantlet regeneration in pecan (Carya illinoensis). Plant Sci 64: 193-201 https://doi.org/10.1016/0168-9452(89)90024-1
  22. Wilde HD, Meagher RB, Merkle SA (1992) Expression of foreign genes in transgenic yellow-poplar plants. Plant Physiol 98: 114-120 https://doi.org/10.1104/pp.98.1.114

Cited by

  1. Effects of ABA, reduced nitrogen source and osmoticum for somatic embryogenesis in Liriodendron tulipifera vol.38, pp.2, 2011, https://doi.org/10.5010/JPB.2011.38.2.186
  2. Comparison of Physiological Characteristics, Stomata and DNA Content between Seedling and 5-year-old Somatic Plant (Somatic Embryo Derived-plant) in Liriodendron tulipifera vol.102, pp.4, 2013, https://doi.org/10.14578/jkfs.2013.102.4.537
  3. Allometric Equations and Biomass Expansion of Yellow Poplar(Liriodendron tulipifera) in Southern Korea vol.105, pp.04, 2016, https://doi.org/10.14578/jkfs.2016.105.4.463
  4. Effects ofin vitroculture types on regeneration and acclimatization of yellow poplar (Liriodendron tulipiferaL.) from somatic embryos vol.43, pp.1, 2016, https://doi.org/10.5010/JPB.2016.43.1.110
  5. Carbon dioxide absorption for Liriodendron tulipifera using fertilization vol.59, pp.4, 2016, https://doi.org/10.1007/s13765-016-0201-8