Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Endophytic Bacillus sp. CY22 from a Balloon Flower (Platycodon grandiflorum) Produces Surfactin Isoforms

  • Cho, Soo-Jeong (Research Institute of Life Science, Gyeongsang National University) ;
  • Hong, Su-Young (Division of Applied Life Science, Gyeongsang National University) ;
  • Kim, Jin-Young (Proteome Analysis Team, Korea Basic Science Institute) ;
  • Park, Sang-Ryeol (Division of Applied Life Science, Gyeongsang National University) ;
  • Kim, Min-Keun (Division of Applied Life Science, Gyeongsang National University) ;
  • Lim, Woo-Jin (Division of Applied Life Science, Gyeongsang National University) ;
  • Shin, Eun-Chule (Division of Applied Life Science, Gyeongsang National University) ;
  • Kim, Eun-Ju (Division of Applied Life Science, Gyeongsang National University) ;
  • Cho, Yong-Un (Department of Microbiological Engineering, Jinju National University) ;
  • Yun, Han-Dae (Division of Applied Life Science and Research Institute of Life Science, Gyeongsang National University)
  • Published : 2003.12.01
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Abstract

Surfactin is a mixture of cyclic lipopeptides built from variants of a heptapeptide and a ${\beta}-hydroxy$ fatty acid produced by several strains of Bacillus sp. Surfactin isoforms produced by endophytic Bacillus sp. CY22 from a balloon flower were isolated and characterized. It was found that the purified surfactin had three isoforms with protonated masses of m/z 1,008, 1,022, and 1,036, and different structures in combination with Na, K, Ca ions using MALDI-TOF MS, ESI-MS/MS, and ICP MS, respectively. In the MS/MS analysis, the isolated surfactin had the identical amino acid sequence (LLVDLL) and hydroxy fatty acids (with 13 to 15 carbons in length), even though isolated from different Bacillus strains. The sfp22 gene, required for producing the surfactin, consisted of an open reading frame (ORF) of 675 bp encoding 224 amino acid residues with a signal peptide of 20 amino acids. The predicted amino acid sequence of sfp22 was very similar to that of Ipa-8.

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References

  1. Biochem. Biophys. Res. Commun. v.31 Surfactin, a crystalline peptide-lipid surfactant produced by Bacillus subtilis: Isolation, characterization and its inhibition of fibrin clot formation Arima,K.;A.Kakinuma;G.Tamura https://doi.org/10.1016/0006-291X(68)90503-2
  2. Biochem. Biophys. Res. Commun. v.188 Identification of amino acid substitutions in the lipopeptide surfactin using 2D NMR spectroscopy Baumgart,F.;B.Kluge;C.Ullrich;J.Vater;D.Ziessow
  3. J. Gen. Microbiol. v.6 Nature and properties of a catalytic agent produced by Bacillus subtilis Bernhimer,A.W.;L.S.Avigad
  4. Biopolymers v.34 Solution three-dimensional structure of surfactin: A cyclic lipopeptide studied by ¹H-NMR, distance geometry, and melecular dynamics Bonmatin,J.M.;M.Genest;H.Labbe;M.Ptak https://doi.org/10.1002/bip.360340716
  5. Lett. Peptide Sci. v.2 Production, isolation and characterization of [Leu4]- and [Ile4]-surfactins from Bacillus subtilis Bonmatin,J.M.;H.Babbe;I.Grangemard;F.Peypoux;R.Maget-Dana;M.Ptak;G.Miche;l https://doi.org/10.1007/BF00122922
  6. Biosci. Biotechnol. Biochem. v.66 Endophytic Bacillus sp. isolated from the interior of balloon flower root Cho,S.J.;S.R.Park;M.G.Kim;W.J.Lim;S.K.Ryu;C.L.An;S.Y.Hong;H.Y.Lee;S.G.Jeong;Y.U.Cho;H.D>Yun https://doi.org/10.1271/bbb.66.1270
  7. J. Microbiol. Biotechnol. v.11 Identification of total extracellular fibrinase from Bacillus sp. W using one- or two-dimensional fibrin zymography for proteomic approcach Choi,N.S.;J.N.Lee;K.S.Yoon;K.Y.Han;S.H.Kim
  8. J. Peptide Sci. v.30 Lipopetides with improved properties: Structure by NMR, purification by HPLC and structure-activity relationships of new isoleucyl-rich surfactins Grangemard,I.;F.Peypoux;J.wallach;B.C.Das;H.c.Labbe;A.Caille;M.Genest;R.Maget-Dana;M.Ptak;J.M.Bonmatin
  9. J. Bacteriol. v.175 Isolation and characterization of Bacillus subtilis genes involved in siderophore biosynthesis: Relationship between B. subtilis sfp0 and Escherichia coli entD genes Grossman,T.H.;M.Tuckma;s.Ellestad;M.S.Osburne https://doi.org/10.1128/jb.175.19.6203-6211.1993
  10. J. Micriobiol. Biotechnol. v.10 Nutrient amendments influence endophytic colonization of rice by Serratia marcesces IRBG500 and Herbaspirillum seropedicae Z67 Gyaneshwar,P.;M.R.Pallavolu;K.LJagdish
  11. Thesis, Universite de Liege Hbrid,C.
  12. J. Gen. Appl. Microbiol. v.38 Characterization of Bacillu ssubtilis RB14, coproducer of peptide antibiotics iturin A and surfactin Hiraoka,H.;O.Asaka;T.Ano;M.Shoda https://doi.org/10.2323/jgam.38.635
  13. Takeda Kenkyusho Ho. v.30 Hyprocholesterolemic effect of surfactin, a novel bacterial peptide lipid Imai,Y.;H.Sugino;K.Takeshi;A.Takinuma
  14. Chem. Pharm. Bull. (Tokyo) v.42 Structural and conformational studies of [Ile7] and [Leu7] surfactins form Bacillus subtilis natto Itokawa,H.;T.Miyashita;H.Morita;K.Takeya;T.Hirano;M.Homma;K.Oka https://doi.org/10.1248/cpb.42.604
  15. Agric. Biol. Chem. v.33 determination of amino acid sequence in surfactin, a crystalline peptidolipid surfactant produced by Bacillus subtilis Kakinuma,A.;M.Hori;M.Isono;g.Tamura;K.Arima https://doi.org/10.1271/bbb1961.33.971
  16. Agric. Biol. Chem. v.33 Determination of fatty acid in surfactin and elucidation of the total structure of surfactin Kakinuma,A.;H.Sugino;M.Isono;G.Tamura;K.Arima https://doi.org/10.1271/bbb1961.33.973
  17. Chem. Pharm. Bull. (Tokyo) v.20 Antitumor activity of Bacillus natto. Ⅲ. Isolation and characterization of a cytolytic substance on Ehrlich ascites carcinoma cells in the culture medium of Bacillus natto KMD 1126 Kameda,Y.;K.Matsui;K.Hisato;T.Yamada;H.Sagai https://doi.org/10.1248/cpb.20.1551
  18. J. Bacteriol. v.183 Cloning, equencing, and characterization of the inturin A operon Kenji,T.;T.Akiyarna;M.Shoda https://doi.org/10.1128/JB.183.21.6265-6273.2001
  19. J. Microbiol. Biotechnol. v.12 Cloning and expression of the aminopeptidase gene from the Bacillus licheniformis in Bacillus subtilis Kim,J.S.
  20. J. Microbiol. Biotechnol. v.9 Control of both foam and dissolved oxygen in the pesence of a surfactant for production of β-carotene in Blakeslea trispora Kim,S.W.;I.Y.Lee;J.C.Jeong;J.H.Lee;Y.H.Park
  21. J. Microbiol. Biotechnol. v.4 Antifungal mechanism and properties of antibiotic substances produced by Bacillus subtilis YB-70 as a biological control agent Kim,Y.S.;S.D.Kim
  22. J. Colloid Interface Sci. v.204 Separation and characterization of surfactin isoforms produced by Bacillus subtilis OKB 105 Kowall,M.;J.Vater;B.Kluge;T.Stein;P.Franke;D.Ziessow https://doi.org/10.1006/jcis.1998.5558
  23. Nature v.390 The complet genome sequence of the gram-positive bacterium Bacillus subtilis Kunst,F.(et al.) https://doi.org/10.1038/36786
  24. Chem. Biol. v.3 A new enzyme superfamily - the phosphopantetheinyl transferases Lambalot,R.H.;A.M.Gehring;R.S.Flugel;P.Zuber;M.LaCelle;M.A.Marahie;R.Reid;C.Khosla;C.T.Walsh https://doi.org/10.1016/S1074-5521(96)90181-7
  25. J. Microbiol. Biotechnol. v.12 Streptomyces with antifungal activity aganist rice blast causing fungus, Magnaporthe grisea Lee,C.H.
  26. Mol. Gen. Genet. v.232 Isolation and characterization of sfp: A gene that funcations in the production of the lipopeptide biosurfactant, surfactin, in Bacillus subtilis Nakano,M.M.;N.Corbell;J.Besson;P.Zuber
  27. Biotechnol. Bioeng. v.47 Production of a lipopeptide antibiotic, surfactin, by recombinant Bacillus subtilis in solid state fermentation Ohno,A.;T.Ano;M.Shoda https://doi.org/10.1002/bit.260470212
  28. Eur. J. Biochem. v.202 Isolation and characterization of a new variant of surfactin, the [Val7] surfactin Peypoux,F.;J.M.Bonmatin;H.Labbe;B.C.Das;M.Ptak;G.Michel https://doi.org/10.1111/j.1432-1033.1991.tb16349.x
  29. Eur. J. Biochem. v.224 [Ala4] surfactin, a novel isoform Bacillus subtilis studied by mass and NMR spectroscopies Peypoux,F.;J.M.Bonmatin;H.Labbe;I.Grangemard;B.C.Das;M.Ptak;J.Wallach;G.Michel https://doi.org/10.1111/j.1432-1033.1994.tb19998.x
  30. Appl. Microbiol. Biotechnol. v.36 Controlled biosynthesis of [Val7]- and [Leu7]-surfactins Peypoux,F.;G.Michel
  31. J. Microbiol. Biotechnol. v.10 Screening and identification of an antifungal Pseudomonas sp. that suppresses balloon flower root rat rot caused by Rhizoctonia solani Ryu,J.S.;S.D.Lee;Y.H.Lee;S.T.Lee;D.K.Kim;S.J.Cho;S.R.Park;D.W.Bae;K.H.Park;H.D.Yun
  32. Molecular cloning: A Laboratory Manual(3rd ed.) Sambrook,J;D.W.Russell
  33. Arch. Microbiol. v.169 Genetic evidence for a role of thioesterase domains, integrated in a associated with peptide synthetases, in non-ribosomal peptide biosynthesis in Bacillus subtilis Schneider,A.;A.M.Marahiel https://doi.org/10.1007/s002030050590
  34. Biochim. Biophys. Acta v.1064 Ionic channels induced by surfactin in planar lipid bilayer membranes Sheppard,J.D.;C.Jumarie;D.G.Cooper;R.Laprade https://doi.org/10.1016/0005-2736(91)90406-X
  35. Colloids Surf. B. v.1 Ionophorous and sequestering properties of surfactin, a biosurfactant from Bacillus subtilis Thimon,L.;F.Peypoux;J.Wallach;G.Michel https://doi.org/10.1016/0927-7765(93)80018-T
  36. Appl. Environ. Microbiol. v.61 Purificatoion and structure determination of an inhibitor of starfish oocyte maturation from a Bacillus sp Toraya,T.;T.Maoka;H.Tsuji;M.Kobayashi
  37. Arch. Microbiol. v.165 Isolation of a gene essential for biosynthesis of the lipopeptide antibiotics plipastatin B1 and surfactin in Bacillus subtilis YB8 Tsuge,K.;T.Ano;M.Shoda https://doi.org/10.1007/s002030050322
  38. Biologicals v.25 Mechanism of inactivation of enveloped virues by the biosurfactant surfactin from Bacillus subtilis Vollenbroich,D.;M.Ozel;J.Vater;R.M.Kamp;G.Pauli https://doi.org/10.1006/biol.1997.0099