Determination of C3G Content in Blackish Purple Rice using HPLC and UV-Vis Spectrophotometer

  • Ryu, Su-Noh (Dept. of Agricultural Sci. Korea Nat'l Open Univ.) ;
  • Park, Sun-Zik (Dept. of Agricultural Sci. Korea Nat'l Open Univ.) ;
  • Kang, Sam-Sik (National Products Research Institute, Seoul Nat'l Univ.) ;
  • Han, Sang-Jun (National Products Research Institute, Seoul Nat'l Univ.)
  • Published : 2003.12.01

Abstract

Cyanidin 3-glucoside (C3G) content contained in the grains of blackish purple rice varieties, Heugjinjubyeo, Kilimheugmi, Heugnambyeo, Sanghaehy-anghyeolla, and the progenies derived from their crosses was evaluated by HPLC and UV-Vis spectroscopy. C3G content was higher in the range of 10-30% by using UV-Vis method compared to HPLC method. A significant linear relationship was, however, observed between two analytical methods. The correlation coefficient was 0.98. Thus, this results suggested that it would be able to use UV-Vis spectroscopy to determine C3G content which does not demanded precise value like selection.

Keywords

References

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