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The Pharmaceutical Society of Korea (대한약학회)
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Inhibitory Effects of Luteolin Isolated from Ixeris sonchifolia Hance on the Proliferation of HepG2 Human Hepatocellular Carcinoma Cells

  • Yee, Su-Bog (Department of Pharmacy, Pusan National University) ;
  • Lee, Jung-Hwa (Department of Pharmacy, Pusan National University) ;
  • Chung, Hae-Young (Department of Pharmacy, Pusan National University) ;
  • Im, Kwang-Sik (Department of Pharmacy, Pusan National University) ;
  • Bae, Song-Ja (Department of Food and Nutrition, Silla University) ;
  • Choi, Jae-Soo (Faculty of Food Science and Biotechnology, Pukyong National University) ;
  • Kim, Nam-Deuk (Department of Pharmacy, Pusan National University)
  • Published : 2003.02.01
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Abstract

We investigated the anti-proliferative effects of luteolin and apigenin, isolated from Ixeris sonchifolia Hance, on HepG2 human hepatocellular carcinoma cells. In MTT assay luteolin showed more efficient anti-proliferative effects on cells than apigenin did. According to propidium iodide staining and flow cytometry studies, we postulated that these effects might be a result of cell cycle arrest. Hence we examined the changes of protein expressions related to cell cycle arrest. Western blotting data demonstrated that the down-regulated expression of CDK4 was correlated to the increase of p53 and CDK inhibitor $p21^{WAF1/CIP1}$ protein. These data suggest that luteolin may have potential as an anti-cancer agent.

Keywords

References

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