Archives of Pharmacal Research

The Pharmaceutical Society of Korea (대한약학회)
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Herbal Extract Prevents Bone Loss in Ovariectomized Rats

  • Kim, Chung-Sook (Drug Research and Development Team, Korea Institute of Oriental Medicine) ;
  • Ha, Hye-Kyung (Drug Research and Development Team, Korea Institute of Oriental Medicine) ;
  • Lee, Je-Hyun (Drug Research and Development Team, Korea Institute of Oriental Medicine) ;
  • Kim, Jin-Sook (Drug Research and Development Team, Korea Institute of Oriental Medicine) ;
  • Song, Kye-Yong (College of Medicine, Chung-Ang University) ;
  • Park, Sie-Won (Deparlment of Chemistry, Sangmyung University)
  • Published : 2003.11.01
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Abstract

This research aims to test a new drug candidate based on a traditional medicinal herb, F1, an herbal extract obtained from Astragalus membranaceus and its main ingredient, 1-monolinolein that may have fewer side effects and less uterine hypertrophy. In vitro experiments, human osteoblast-like cell lines, MG-63 and Saos-2, were analyzed by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) and an alkaline phosphatase (ALP) assays. Mouse osteoclasts were induced through a calcium-deficient diet and inhibition effects were measured. In vivo experiments were done using ovariectomized (OVX) rats for 9 weeks. At necropsy, uterus weights were measured, trabecular bone area (TBA) of tibia and lumbar vertebra were measured bone histomorphology. In results, cell proliferation and ALP activity in Saos-2 by ether F1 or 1-monolinolein did not increased significantly compared to the control. The F1 inhibited osteoclast development ($IC_{25}=3.37{\times}10^{-5}$mg/mL) less than 17$\beta$-estradiol. The OVX rats administered F1 (2 mg/kg/day and 10 mg/kg/day) showed an increase in TBA of the tibia significantly (136.3${\pm}4.2% and 138.5{\pm}$10.3% of control). In conclusions, the herbal extract, F1 inhibited tibia and lumbar bone loss and did not cause uterine hypertrophy. However, 1-monolinolein, the main ingredient of the herbal extract, did not inhibit bone loss.

Keywords

References

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