MBR 공정에 있어서 미생물 성장특성과 SMP의 거동

Characteristics of Cell-Growth and Behavior of SMP in the MBr Process

차기철;황명구;정형근;김동진;유익근;한상국
Cha, Gi Cheol;Hwang, Myeong Gu;Jeong, Hyeong Geun;Kim, Dong Jin;Yu, Ik Geun;Han, Sang Guk

  • 발행 : 20030000

초록

실험실 규모의 침지형 MBR 공정을 통하여 막투과성능의 변화 및 막오염과 미생물 활성 등 영향인자로서의 용해성 생물 대사산물(soluble microbial products, SMP)의 생성특성을 살펴보았으며, 반응기내 미생물 성장의 동력학적 해석을 시도하였다. 기질은 물질이동의 정확한 매카니즘의 학인을 위해 페놀을 단일 탄소원으로 하는 인공기질을 사용하였다. 초기 미생물농도는 약 3500g MLSS/L로 하였으며, HRT는 12시간으로 하였다. 실험결과, 질화미생물에 활성은 반응조내 생물대사산물의 농도변화에 상당한 영향을 받는 것을 확인할 수 있었으며, 분리막의 투과능력 또한 생물대사산물의 농도변화에 크게 반비례하는 것을 확인할 수 있었다. 이는 세포세척(cell washing)을 통해 생물대사산물의 농도를 감소시켰을 때 막의 투과능력이 급격히 상승하는 것을 통해 다시 확인할 수 있었으며, $SMP_t$$T_{max}$와의 관계를 살펴본 결과 SMP 농도가 증가할수록 $T_{max}$는 지수함수적으로 감소하는 것을 확인할 수 있었다. 한편, 수학적 모델식을 적용하여 미생물 동력학적 인자를 도출해본 결과 비정상상태에서의 증식수율은 0.447mg/MLVSS/mg TOC로 나타났으며, 사멸율은 0.0084 $day^{-1}$로 나타났다. 또한 정상상태에서의 증식수율과 사멸율은 각각 0.115, 0.118로 나타났으며, 본 실험 조건에서의 최대 비증식율$\mu^{max}$은 0.068 $day^{-1}$로 나타났다.

Lab-scale submerged membrane bio-reactor (MBR) process with intermittent aerobic condition was installed to investigate the characteristic of kinetics of cell-growth, and observe the relationship between soluble microbial products (SMP) with membrane permeability and/or microbial activity in the system. The effective volume of reactor was 12 L. The hydraulic retention time (HRT) and flux were 12 h and 10 L/㎡/hr, respectiverly. Synthetic substrate (carbon source: phenol) was used to investigate the mechanism of cell growth. As a results of experiment, the activity of nitrifier was influenced on the concentration of SMP in the mixed liquor, and SMP also affected the permeability of membrane. It was re-confirmed from the drop of the SMP concentration in the mixed liquor using a cell washing (mingled mixed liquor with 0.1 N NaCl and then separate the supernatant). After cell washing, the permeability of membrane was recovered. From mixed liquor with 0.1 N NaCl and then separate the supernatant). After cell washing, the permeability of membrane was recovered. From the comparison between $SMP_t$ and $T_{max}$, the $T_{max}$ was decreased exponentially with increase of $SMP_t$ concentraion. The values of Y and decay rate in the unsteadys-tate period were 0.0447 mg MLSS/mg TOC and 0.0084 $day^{-1}$, respectively, In the steady-state period, they were 0.115 mg MLVSS/mg TOC and 0.118 $day^{-1}$, respectively. The maximum growth rate in the stead-state period was 0.068 $day^{-1}$.

키워드

참고문헌

  1. 대한환경공학회지 v.23 no.11 활성슬러지 공정에서 호기-무산소 조건변화에 따른 생물대사산물(SMP)의 분자량 분포특성 차기철;황명구;정형근;김동진
  2. First IWA Asia-Pacific Regional Conference 2001, Proceedings Ⅱ Influence of intermittent aeration on the flux and soluble microbial products (SMP) in a membrane separation activated sludge process Hwang, M. G.;Cha, G. C.;Chung, H. K.;Kim, D. J.
  3. Water Res. v.36 no.16 Inert COD production in a membrane anaerobic reactor treating brewery wastewater Kasapgil Ince, B.;Ince, O.;Sallis, P. J.;Anderson, G. K.
  4. Water Science & Technology v.34 no.9 Influence of bacterial extracellular polymers on the membrane separation activated sludge process Nagaoka, H.;Ueda, S.;Miya, A. https://doi.org/10.1016/S0273-1223(96)00800-1
  5. 대한환경공학회지 v.23 no.2 침지형 막분리 할성 슬러지 법에 있어서 생물대사산물의 동력학적 특성 차기철;이동열;정형근;김동진
  6. 酸酵工學會誌 v.67 no.2 ヒルロ-ス系廢水の嫌氣性消化たおける動力學的解析 柏分英助;前田嘉道;菅健一
  7. Bioresource Technology v.57 no.1 Modeling of volatile fatty acids degradation kinetics and evaluation of microorganism Vavilin, V. A.;Lokshina, Y. https://doi.org/10.1016/0960-8524(96)00052-1
  8. 日本 土木學會論文集 v.461 no.Ⅱ-22 低溫域における嫌氣性酸醱酵の動力學的特性に及ぼす溫度の影響 車基喆;李玉友;野池達也
  9. Water Sci. Technol. v.39 Nitrogen removal by submerged membrane separation activated sludge process Nagaoka, H.
  10. 日本 水環境工學硏究論文集 v.20 no.7 膜分離活性汚泥法における膜透過性能に對する生物代謝成分の影響 柳根勇;木非井至;小松俊
  11. Standard Methods for the Examination of Water and Wastewater(17th ed.) APHA;AWWA;WPCF
  12. Water Research v.35 no.8 A model for membrane bioreactor process based on the concept of formation and degradation of soluble microbial products Lu, S. G.;Imai, T.;Ukita, M.;Sekine, M.;Higuchi, T.;Fukagawa, M. https://doi.org/10.1016/S0043-1354(00)00461-9
  13. Water Science & Technology v.34 no.5-6 Molecular weight distribution of soluble microbial products in biological systems Boero, V. J.;Bowers, A. R.;Eckenfelder, Jr. W. W. https://doi.org/10.1016/0273-1223(96)00651-8
  14. Water Research v.19 no.1 Quantitative estimation in COD units of refractory organic compounds produced by activated sludge microorganisms Chudoba. J. https://doi.org/10.1016/0043-1354(85)90320-3
  15. Water Research v.33 no.14 A review of soluble microbial products (SMP) in wastewater treatment systems Barker, D. J.;Stuckey, D. C. https://doi.org/10.1016/S0043-1354(99)00022-6
  16. Water Science & Technology v.38 no.4 Advanced analysis of membrane bioreactor performance with aerobic-anoxic cycling Silva, D. C. G. V.;Urbain, V.;Abeysinghe, D. H.;Rittmann, B. E. https://doi.org/10.1016/S0273-1223(98)00551-4
  17. 대한환경공학회지 v.22 no.9 침지형 막분리 활성슬러지법에 있어서 생물대사성분과 막오염의 특성 차기철;이동열;유익근;김동진;심진기
  18. 日本水質汚染硏究 v.3 好氣性消火法の有機物除法機構に及ぼす消化溫度の影響に關する硏究 李 宅渟ほか
  19. 日本下水道協會誌 v.14 no.161 活性汚泥法の淨化機構に及ぼす水溫の影響に關する動力學的硏究 (第 2報) -Monod型動力學式による回分實驗の水溫影響解析 橋本 鳥山明夫