Mycobiology

The Korean Society of Mycology (한국균학회)
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Screening for Antifungal Endophytic Fungi Against Six Plant Pathogenic Fungi

  • Park, Joong-Hyeop (Biological Function Research Team, Korea Research Institute of Chemical Technology) ;
  • Park, Ji-Hyun (Biological Function Research Team, Korea Research Institute of Chemical Technology) ;
  • Choi, Gyung-Ja (Biological Function Research Team, Korea Research Institute of Chemical Technology) ;
  • Lee, Seon-Woo (Biological Function Research Team, Korea Research Institute of Chemical Technology) ;
  • Jang, Kyoung-Soo (Biological Function Research Team, Korea Research Institute of Chemical Technology) ;
  • Choi, Yong-Ho (Biological Function Research Team, Korea Research Institute of Chemical Technology) ;
  • Cho, Kwang-Yun (Biological Function Research Team, Korea Research Institute of Chemical Technology) ;
  • Kim, Jin-Cheol (Biological Function Research Team, Korea Research Institute of Chemical Technology)
  • Published : 2003.09.30
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Abstract

A total of 187 endophytic fungi were isolated from 11 plant species, which were collected from 11 locations in Korea. Their antifungal activities were screened in vivo by antifungal bioassays after they were cultured in potato dextrose broth and rice solid media. Antifungal activity against plant pathogenic fungi such as Magnaporthe grisea(rice blast), Corticium sasaki(rice sheath blight), Botrytis cinerea(tomato gray mold), Phytophthora infestans(tomato late blight), Puccinia recondita(wheat leaf rust), and Blumeria graminis f. sp. hordei(barley powdery mildew) was determined in vivo by observing the inhibition of plant disease development. Twenty(11.7%) endophytic fungi fermentation broths were able to control, by more than 90%, at least one of the six plant diseases tested. Among 187 liquid broths, the F0010 strain isolated from Abies holophylla had the most potent disease control activity; it showed control values of more than 90% against five plant diseases, except for tomato late blight. On the other hand, fourteen(7.5%) solid culture extracts exhibited potent disease control values of more than 90% against one of six plant diseases. The screening results of this study strongly suggested that metabolites of plant endophytic fungi could be good potential sources for screening programs of bioactive natural products.

Keywords

References

  1. Anke, T., Oberwinkler, E, Steglich, W. and Schramm, G. 1977. The strobilurins-new antifungal antibiotics from the Basidiomycete Strobilurus tenacellus. J. Antibiot. 30: 806-810
  2. Arima, K., Imanaka, H., Kousaka, M., Fukuda, A. and Tamura, C. 1964. Pyrrolnitrin, a new antibiotic substance, produced by Pseudomonas. Agric. Biol. Chem. 28: 575-576 https://doi.org/10.1271/bbb1961.28.575
  3. Carroll, G. C. 1988. Fungal endophytes in stems and leaves: from latent pathogen to mutualistic symbiont. Ecology 69: 2-9 https://doi.org/10.2307/1943154
  4. Collado, J., Platas, G. and Pelaez, E 1996. Fungal endophytes in leaves, twings and bark of Quercus ilex from Central Spain. Nova Hedwigia 63: 347-360
  5. Fisher, P. J., Anson, A. E. and Petrini, O. 1984. Antibiotic activity of some endophytic fungi from ericaceous plants. Botanica Helvetica 94: 249-253
  6. Huang, Y, Wang, J., Li, G., Zheng, Z. and Su, W. 2001. Antitumor and antifungal activities in endophytic fungi isolated from pharmaceutical plants Taxus mairei, Cephalataxus fortunei and Torreya grandis. FEMS lmmunol. Med. Microbiol. 31: 163167
  7. Kim, J.-C., Choi, G. I., Park, J.-H., Kim, H. T. and Cho, K. Y 2001. Activity against plant pathogenic fungi of phomalactone isolated from Nigrospra sphaerica. Pest Manag. Sci. 57: 554-559 https://doi.org/10.1002/ps.318
  8. Lee, I. C., Lobkovsky, E., Pliam, N. B., Stroble, G. A. and Clardy, J. 1995. Subglutinol A and B: inununosuppressive compounds from the endophytic fungus Fusarium subglutinans. J. Org. Chem. 60: 7076-7077 https://doi.org/10.1021/jo00127a001
  9. Musilek, V., Cerna, J., Sasek, V., Semerdzieva, M. and Vondracek, M. 1969. Antifungal antibiotic of the Basidiomycete Oudemansiella mucida. Folia Microbiologica (Prague) 14: 377-387 https://doi.org/10.1007/BF02872707
  10. Pelaez, E, Collado, I., Arenal, E, Basilio, A., Cabello, A., Diez Matas, M. T., Garcia, J. B., Gonzalez Del Val, A., Gonzalez, V., Gorrochategui, J., Hernandez, P., Martin, I., Platas, G. and Vicente, E 1998. Endophytic fungi from plants living on gypsum soils as a source of secondary metabolites with antimicrobial activity. Mycol. Res. 102: 755-761 https://doi.org/10.1017/S0953756297005662
  11. Stierle, A., Strobel, G. A. and Stierle, D. 1993. Taxol and taxane production by Taxomyces andreanae, an endophytic fungi of Pacific yew. Science 260: 214-216 https://doi.org/10.1126/science.8097061
  12. Strobel, G. A. and Long, D. M. 1998. Endophytic microbes embody pharmaceutical potential. ASM News 64: 263-268
  13. Stroble, G. A. and Hess, W. M. 1997. Glucosylation of the peptide leucinostatin A, produced by an endophytic fungus of European yew, may protect the host from leucinostatin toxicity. Chem. Biol. 4: 529-536 https://doi.org/10.1016/S1074-5521(97)90325-2
  14. Wang, J., Li, G., Lu, H. Zheng, Z., Huang, Y and Su, W. 2000. Taxol from Tubercularia sp. strain TF5, an endophytic fungus of Taxus mairei. FEMS Microbiol. Lett. 193: 249-253

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