Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
권호 표지

Mass Production of Adventitious Roots of Eleutherococcus sessiliflorus through the Bioreactor Culture

  • Seo Jin-Wook (Department of Biotchnology, Chung-Ang University) ;
  • Shin Cha-Gyun (Department of Biotchnology, Chung-Ang University) ;
  • Choi Yong-Eui (Korea Ginseng Institute, Chung-Ang University)
  • Published : 2003.09.01
Download PDF
⟨ Previous Next ⟩

Abstract

This paper reported the establishment of mass production system of adventitious roots of Eleutherococcus sessiliflorus through the shake flask and bio-reactor culture. Induction of adventitious roots was started from the explants of germinated somatic embryos on half-strength Murashing and Skoog (MS) solid medium. The frequency of adventitious root formation was better in the explants comprising the basal hypocotyl parts than root explants alone. Among the different auxins tested (NAA, IBA and IAA), frequency of adventitious root induction was highest on medium with 0.5 mg/L NAA, and produced $16.3\pm1.9$ roots per explant. In shake-flask culture, deletion of $NH_4NO_3$ of MS medium was effective for induction of adventitious root compared with both full and half-strength MS media. Fresh weight increase of induced adventitious roots was performed well in medium with 0.5 mg/L IBA. When adventitious roots produced in shake-flask culture were transferred to 10-liter bioreactor, 5.5 times of fresh weight increase was gained after one month of culture. HPLC analysis revealed that the amount of eleutheroside E and E1 was higher in in vitro cultured adventitious roots than the 3 year-old field cultivated root barks of Eleutherococcus sessiliflorus. The content of eltutheroside B was much lower in adventitious roots than that of field cultivated one.

Keywords

References

  1. Korean J Ginseng Sci v.16 Study of the propagation of Acanthopanax plants.1.Effect of growth regulators on rooting in stem cuttings Ahn,S.D.;Choi,E.O.
  2. Annu Rev Pharmacol v.9 new substances of plant origin which increase nonspecific resistance Brekhman,Ⅱ.;Dardymov,Ⅳ.
  3. Plant Growth Regulation v.10 The role of endogenous auxin in root initiation.PartⅠ.Evidence from studies on auxin application and analysis of endogenous levels Blakesley,D.;Weston,G.D.;Hall,J.F.
  4. Ann Bot v.83 High frequency of plant production via somatic embryogenesis from callus or cell suspension cultures in Eletherococcus senticosus Choi,Y.E.;Kim,J.W.;Yoon,E.S.
  5. Plant Cell Tiss Org Cult v.58 Rapid Propagation of Eleutherococcus senticosus via direct somatic embryogenesis from explants of germinating zygotic embryos Choi,Y.E.;Yang,D.C.;Yoon,E.S.
  6. Plant Cell Rep v.18 High-efficiency plant production via direct somatic single embryogenesis from preplasmolysed cotyledons of panax ginseng and possible domancy of somatic embryos Choi,Y.E.;Yang,D.C.;Yoon,E.S.;Choi,K.T.
  7. Plant Cell Tiss Org Cult v.69 Production of Plantlets of Eleutherococcus sessiliflorus via somatic embryogenesis and successful transfer to soil Choi,Y.E.;Ko,S.K.;Lee,K.S.;Yoon,E.S.
  8. Plant Cell Rep v.19 Production of transgenic plants via Agrobacterium rhizogenes-mediated transformation of Panax ginseng Choi,Y.E.;Yang,D.C.
  9. J Plant Biol v.40 Effect of Ammonium ion on Morphogenesis from Cultured Cotyledon Explants of Panax ginseng Choi,Y.E.;Woong,Y.S.
  10. Plant Cell Tiss Org Cult v.62 Pilot-scale cultures of adventitious roots of ginseng in a bioreactor system Choi,S.M.;Son,S.H.;Yun,S.R.;Paek,K.Y.
  11. Plant Growth Regulation v.27 In vitro root culture of Panax ginseng and P.Quinquefolium Kevers,C.;Jacques,Ph.;Thonart,Ph.;Gaspar,Th.
  12. Plant Cell Rep v.9 Somatic embryogenesis and plant regeneration in Acanthopanax senticosus Gui,Y.;Guo,Z.;Ke,S.;Skirvin,R.H.
  13. Nat Med v.48 Studies on the cultivation of Eleutherococus senticosus Maxim.Ⅱ On the germination and rising of seedling Osoda,S.;Shoji,J.
  14. Korean J Pharmocology v.10 Distribution of Acanthopanax plants in Korea Lee,W.T.
  15. Physiol Plant v.15 A revised medium for rapid growth and bioassays with tobacco tissue cultures Murashige,T.;Skoog,F.
  16. Adversive Effects of Herbal Drugs v.2 Eleutherococcus senticosus Sonnenborn,U.;Hansel,R.;DeSmet,P.A.G.M.(ed);Keller,K.(ed);Hansel,R.(ed),Chandler,R.F.(ed)