Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Effects of the Water Extract from Albizzia julibrissin on Apoptosis and Cell Cycle Progression in the Human Leukemic Jurkat Cells

백혈병 세포주 Jurkat의 세포고사 유도 및 세포주기 억제에 미치는 합산피 추출물의 효과

  • Park Young Jun (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Jung Woo Cheal (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Jeong Dae Young (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Lee Yong Un (Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Lee In (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Lee Key Sang (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Jeon Byung Hun (Department of Pathology, College of Oriental Medicine, Wonkwang University) ;
  • Sung Kang Keyng (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Moon Byung Soon (Professional Graduate School of Oriental Medicine, Wonkwang University)
  • 박영준 (원광대학교 한의과대학 내과학교실) ;
  • 정우철 (원광대학교 한의과대학 내과학교실) ;
  • 정대영 (원광대학교 한의과대학 내과학교실) ;
  • 이용운 (원광대학교 한의학전문대학원) ;
  • 이인 (원광대학교 한의과대학 내과학교실) ;
  • 이기상 (원광대학교 한의과대학 내과학교실) ;
  • 전병훈 (원광대학교 한의과대학 병리학교실) ;
  • 성강경 (원광대학교 한의과대학 내과학교실) ;
  • 문병순 (원광대학교 한의학전문대학원)
  • Published : 2003.12.01
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Abstract

Apoptosis is a morphologically and biochemically district form of cell death that occurs in many different cell types in a wide variety of organisms. Albizzia julibrissin belonging the family Leguminosae has been used for the treatment of contusion, sore throat, amnesia, and insomnia in oriental traditional medicine. This study investigates whether the water extract of A. julibrissin induce apoptotic cell death in Jurkat T-acute lymphoblastic leukemia (ALL) cells. Jurkat cells were increased inhibitions of cell viability in a concentration-dependent manner by A. julibrissin. This herbal medicine also caused apoptosis as measured by cell morphology and DNA fragmentation. The capability of A. julibrissin to induce apoptosis was associated with proteolytic cleavage of specific target proteins such as poly (ADP-ribose)polymerase (PARP) and beta-catenin proteins suggesting the possible involvement of caspases. Our result showed that Bcl-2 and Bax protein levels were not changed in all A. julibrissin-treated groups compared to control group. These results suggest that A. julibrissin-mediated apoptosis is independent with Bcl-2 related signaling pathway in this cells. The purpose of the present study is also to investigate the Effect of A. julibrissin on cell cycle progression. Our results showed that G1 checkpoint related gene products (cyclin D1, cyclin dependent kinase 4, retinoblastoma, E2F1) were decreased in their protein levels in a dose-dependent manners after treatment of the extract. These results indicate that the increase of apoptotic cell death by A. julibrissin may be due to the inhibition of cell cycle progression in wild type p53-lacking Jurkat cells.

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References

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