Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Effects of Water-Extracted Cyperus Rotundus on the Nitric Oxide Production and Cytokine gene Expression

향부자 열수추출물의 Nitric oxide 생성 및 iNOS 유전자 발현에 미치는 영향

  • Lee Young Sun (Efficacy and Safety Research Center for Traditional, Oriental Medicines, Daegu Haany University) ;
  • Han Ok Kyung (Efficacy and Safety Research Center for Traditional, Oriental Medicines, Daegu Haany University) ;
  • Shin Sang Woo (Department of Pathology, College of Oriental Medicine, Daegu Haany University) ;
  • Park Jong Hyun (Department of Pathology, College of Oriental Medicine, Daegu Haany University) ;
  • Kwon Young Kyu (Department of Physiology, College of Oriental Medicine, Daegu Haany University)
  • 이영선 ((재)경북테크노파크, 대구한의대학교 한방생명자원특화센터 효능검증원) ;
  • 한옥경 ((재)경북테크노파크, 대구한의대학교 한방생명자원특화센터 효능검증원) ;
  • 신상우 (대구한의대학교 한의과대학 병리학교실) ;
  • 박종현 (대구한의대학교 한의과대학 병리학교실) ;
  • 권영규 (대구한의대학교 한의과대학 생리학교실)
  • Published : 2003.06.01
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Abstract

The present study was conducted to evaluate the regulation mechanism of nitric oxide(NO) by water-extracted Cyperus rotundus (WCR) in RAW 264.7 macrophages. We investigated the effects of cell proliferation in mouse spleen cell and RAW 264.7 macrophages cells. WCR enhanced mitogenic activity in the dose-response manner in mouse spleen cells and RAW 264.7 macrophages cells. In nitric oxide (NO) synthesis by WCR, WCR alone had an effect on NO synthesis. It was found that the production of NO of RAW 264.7 cells stimulated with lipopolysaccharide (LPS) could be markedly inhibited by WCR. Inhibition of NO production was achieved by reducing inducible nitric oxide syntheses(iNOS) mRNA expression. The expression of IL-I gene by WCR was investigated using reverse transcription polymerase chain reaction (RT-PCR). In RT-PCR, IL-1 family(IL-1 α, IL-1β) expressions were induced by WCR. These finding suggested that regulation of NO production by WCR may be, at least in part, associated with the regulation of iNOS mRNA expression and IL-1 family gene expression.

Keywords

References

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