The Journal of Internal Korean Medicine (대한한방내과학회지)

The Society of Internal Korean Medicine (대한한방내과학회)
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The effect of Injinchunggan-tang(Yinchenqinggan-tang) on Inflammatory Cytokine Gene Expression in Kupffer Cells

인진청간탕(茵蔯淸肝湯)이 kupffer cell 의 inflammatory cytokine 발현에 미치는 영향

  • Kim Ji-Kwon (Department of Internal Medicine, College of Oriental Medicine, Kyung Hee University) ;
  • Kim Young-Chul (Department of Internal Medicine, College of Oriental Medicine, Kyung Hee University) ;
  • Lee Jang-Hoon (Department of Internal Medicine, College of Oriental Medicine, Kyung Hee University) ;
  • Woo Hong-Jung (Department of Internal Medicine, College of Oriental Medicine, Kyung Hee University)
  • 김지권 (경희대학교 한의과대학 간계내과학교실) ;
  • 김영철 (경희대학교 한의과대학 간계내과학교실) ;
  • 이장훈 (경희대학교 한의과대학 간계내과학교실) ;
  • 우홍정 (경희대학교 한의과대학 간계내과학교실)
  • Published : 2004.12.01
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Abstract

Objectives : This study was designed to investigate the effects of Injinchunggan-tang(Yinchenqinggan-tang) on the expression of inflammatory cytokine genes and proteins in kupffer cells. Materials and Methods : The mRNA expression level and protein secretion level were measured using quantitative RT-PCR and ELISA assay respectively in Injinchunggan-tang-treated and untreated kupffer cells after exposed to ethanol, acetaldehyde and lipopolysaccharide. Results : Injinchunggan-tang(Yinchenqinggan-tang) reduced mRNA expression level and protein secretion level of $TNF-{\alpha},\;TGF-{\beta}1,\;IL-1{\beta},\;IL-6,\;IL-8$ that are induced by ethanol, acetaldehyde and lipopolysaccharide in kupffer cells and that mediate inflammation and fibrosis of liver. Conclusion : The result indicates that Injinchunggan-tang (Yinchenqinggan-tang) blocks alcohol-induced liver injury and protects liver by reducing production of inflammatory cytokines.

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