Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
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Preparation of Oligonucleotide Arrays with High-Density DNA Deposition and High Hybridization Efficiency

  • Park, Jeong-Won (Department of Chemistry and Center for Molecular Design and Synthesis, Korea Advanced Institute of Science and Technology) ;
  • Jung, Yong-Won (Department of Chemistry and Center for Molecular Design and Synthesis, Korea Advanced Institute of Science and Technology) ;
  • Jung, Young-Hwan (Department of Chemistry and Center for Molecular Design and Synthesis, Korea Advanced Institute of Science and Technology) ;
  • Seo, Jeong-Sun (Macrogene, Inc., Department of Biochemistry and Molecular Biology, Seoul National University) ;
  • Lee, Young-Hoon (Department of Chemistry and Center for Molecular Design and Synthesis, Korea Advanced Institute of Science and Technology)
  • Published : 2004.11.20
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Abstract

In DNA microarray produced by DNA-deposition technology, DNA-immobilization and -hybridization yields on a solid support are most important factors for its accuracy and sensitivity. We have developed a dendrimeric support using silylated aldehyde slides and polyamidoamine (PAMAM) dendrimers. An oligonucleotide array was prepared through a crosslinking between the dendrimeric support and an oligonucleotide. Both DNAimmobilization and -hybridization yields on the solid support increased by the modification with the dendrimers. The increase of the immobilization and hybridization efficiency seems to result from a threedimensional arrangement of the attached oligonucleotide. Therefore, our dendrimeric support may provide a simple and efficient solution to the preparation of DNA microarrays with high-density DNA-deposition and high hybridization efficiency.

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