The Journal of Internal Korean Medicine (대한한방내과학회지)

The Society of Internal Korean Medicine (대한한방내과학회)
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Study on Apoptosis Effect and Mechanism by Bojungikki-tang on Human Cancer Cell Line H460

폐암세포주(肺癌細胞株) H460에 대(對)한 보중익기탕(補中益氣湯)의 세포고사효과(細胞枯死效果) 및 기전연구(機轉硏究)

  • Lee, Seung-Eon (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Hong, Jae-Eui (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Lee, Si-Hyeong (Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Shin, Jo-Young (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Ro, Seung-Seok (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University)
  • 이승언 (원광대학교 한의과대학 폐계내과학교실) ;
  • 홍재의 (원광대학교 한의과대학 폐계내과학교실) ;
  • 이시형 (원광대학교 한의학 전문대학원) ;
  • 신조영 (원광대학교 한의과대학 폐계내과학교실) ;
  • 노승석 (원광대학교 한의과대학 폐계내과학교실)
  • Published : 2004.12.30
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Abstract

Objectives : This study was designed to evaluate the effect on cytotoxicity of Bojungikki-tang(BIT) in human lung cancer H460 cells. Methods : BIT-induced cell death was confirmed as apoptosis characterized by chromatin condensation and increase of the $sub-G_1$, DNA content. It was tested whether the water extract of BIT affects the cell cycle regulators such as, p2l/Cipl, p27/Kipl, cyclin $B_1$. Results : The data showed that treatment of BIT decreased the viability of H460 cells in a dose-dependent manner. p2l/Cip1 is gradually decreased by the addition of the cells with BIT extract. Interestingly, p27/Kip1 is not detected for 24 hr after the addition of BIT extract, however, after 24 hr, p27/Kipl markedly increased. In addition, cyclin $B_1$, decreased in a time dependent manner after the addition of the water extract. The activation of caspase -3 protease was further confirmed by degradation of procaspase-8 protease andpoly(ADP-ribose) polymerase(P ARP) by BIT in H460 cells. Moreover, BIT induced the increase of Bak expression. Conclusion : These results suggest that the extract of BIT exerts anticancer effects to induce the death of human lung cancer H460 cells via down regulation of cell cycle regulators such as p2l/Cip1, and cyclin B1 or up regulation of cell cycle regulators such as p27/Kip1. Moerover results suggest that BIT induces an apoptosis in H460 cells via activation of intrinsic caspase cascades.

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