Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Comparision of SOD Activity and Phenolic Compound Contents in Various Korean Medicinal Plants

한국산 약용식물로부터의 SOD활성 및 Phenolic Compounds 함량 비교

  • Lim, Jung-Dae (College of Agriculture & Life Sci., Kangwon Natl. Univ.) ;
  • Yu, Chang-Yeon (College of Agriculture & Life Sci., Kangwon Natl. Univ.) ;
  • Kim, Myong-Jo (College of Agriculture & Life Sci., Kangwon Natl. Univ.) ;
  • Yun, Song-Joong (Division of Biological Resources Sci., Chonbuk Natl. Univ.) ;
  • Lee, Sun-Ju (College of Life and Environment Sci., Konkuk Univ.) ;
  • Kim, Na-Young (Department of Food & Nutrition, Kyung Hee Univ.) ;
  • Chung, Ill-Min (College of Life and Environment Sci., Konkuk Univ.)
  • 임정대 (강원대학교 농생대 식물응용과학부) ;
  • 유창연 (강원대학교 농생대 식물응용과학부) ;
  • 김명조 (강원대학교 농생대 식물응용과학부) ;
  • 윤성중 (전북대학교 생물자원과학부) ;
  • 이선주 (건국대학교 생명환경과학대학) ;
  • 김나영 (경희대학교 식품영양학과) ;
  • 정일민 (건국대학교 생명환경과학대학)
  • Published : 2004.06.30
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Abstract

82 species of Korean medicinal plants were measured to their SOD (superoxide dismutase) activity, total phenol and individual phenol compounds. The SOD activity of medicinal plants was varied from 3.67% to 48.3%, and especially, 4 species including Polygala tenuifolia Willd. showed activity over 40% higher than other species. The 16 individual phenol compounds concentrations have a severe variation in medicinal plants. Codonopsis lanceolata is the highest concentration $(12,176\;{\mu}g/g)$, and 10 species including Glycyrrhiza uralensis Fisch were showed to concentrations more than $1,000\;{\mu}g/g$. And also, salicylic acid was generally found in many plants, but trans-cinnamic acid, naringenin and kampferol was little found in plants. The total phenol by Folin-Dennis methods showed a respectively variation $(3{\sim}249.731\;{\mu}g/g)$ in 82 medicinal plants, and 7 species including Astragalus membranaceus showed low total phenol concentrations less than $10\;{\mu}g/g$. In conclusion, we expect that selecting medicinal plants with high biological activity might develop to chemicals related to antioxidants, as well as these become most popular substance at many industrial materials, and suggest to more researchs in future.

한국에 분포하고 있는 82종의 약용식물에 대하여 환경내성 및 기능성 소재의 개발을 위한 탐색을 위하여 항산화효소와 관련된 첫 번째 방어기작인 SOD 효소활성과 Phenol 화합물의 함량정도를 검정하여 본 결과 SOD 효소활성은 원지 (P. tenuifolia Willd.), 백편두 (D. lablab L.), 백개자 (S. alba L.), 대황 (R. palmatum L.), 감초 (G. uralensis Fisch), 연자육 (N. nucifera Gaertn), 지황 [R. glutinosa (Gaertner) Liboshitz], 현삼 (S. buergeriana Miq.), 인진 (A. capillaris Thb.)등에서 다른 약용작물보다 비교적 높은 SOD 효소 활성 능력을 나타냈다. Total phenol함량은 $(3{\sim}249.731\;{\mu}g/g)$의 범위를 보였으며 지유(S. officinalis L.), 대황 (R. palmatum L.), 복분자 (R. chingii Hu)에서 각각 $249.731\;{\mu}g/g,\;209.546\;{\mu}g/g,\;170.333\;{\mu}g/g$으로 가장 높은 함량을 나타냈다. 16종의 phenol 화합물의 경우에서는 82종의 약용식물이 각기 매우 상이한 농도의 분포를 나타내었다. 앞으로의 연구에서는, 항산화 효소 및 phenol 화합물이 많이 함유하여 우수한 기능성을 가지고 있는 것을 확인된 약용식물에 대해 그들의 이용성을 증대시킬 수 있는 구체적인 연구가 수행되어져야 할 것으로 사료된다. 또한 phenol성 물질을 많이 함유하는 약용식물 추출물을 이용한 기능성 식품, 화장품, 천연 보존재 및 의약품 등으로 개발 (Cook & Samman, 1996)할 수 있을 것으로 판단되어진다.

Keywords

References

  1. Alscher RG, Hess JL (1993) Antioxidants in higher plants. CRC Press, Boca Raton p. 1-174
  2. Beyer WF, Fridovich Y (1987) Assaying of superoxide dismutase activity; some large consequences of minor change in condition Analytical Biochemistry 161:559-566 https://doi.org/10.1016/0003-2697(87)90489-1
  3. Brandt K, Molgaard JP (2001) Organic agriculture: Does it enhance or reduce the nutritional value of plant foods? J. Sci. Food AgIic. 81:924-931 https://doi.org/10.1002/jsfa.903
  4. Bryan DM, Murnaghan J, Jones KS, Bowley SR (2000) Ironsuperoxide dismutase expression in transgenic alfalfa increases winter survival without a detectable increase in photosynthetic oxidative stress tolerance. Plant Physiol. 122(4):1427-1438 https://doi.org/10.1104/pp.122.4.1427
  5. Cerutti PA (1985) Prooxidant states and tumor promotion. Science 227:375-381 https://doi.org/10.1126/science.2981433
  6. Cohen G (1988) Oxygen radicals and Parkinson's disease. In 'Oxygen Radicals abd Tissue Injury' (Halliwell B, ed). Federation of American Societies for Experimental Biology, Bethesda, MD. p. 130-135
  7. Duval B, Shetty K, Thomas WH (1999) Phenolic compounds and antioxidant properties in the snow alga Chlamydomonas nivalis after exposure to IN light. J. Appl. Phycol. 11:559-566 https://doi.org/10.1023/A:1008178208949
  8. Foyer CH, lopez-Delgado H, Dat JF, Scott IM (1997) Hydrogen peroxide and glutathione associated mechanism of acclimatory stress tolerance and signalling. Physiol. Plant. 100:241-254 https://doi.org/10.1111/j.1399-3054.1997.tb04780.x
  9. Frankel EN, Waterhouse AL, Kinsella JE (1993) Inhibition of human LDL oxidation by resveratrol. Lancet. 341:1103-1104
  10. Grasbon T, Grasbon-Frodl EM, Juliusson B, Epstein C, Brundin P, Kampik A, Ehinger B (1999) CuZn superoxide dismutase transgenic retinal transplants. Graefes Arch Clin Exp Ophthalmol. 237(4):336-41 https://doi.org/10.1007/s004170050241
  11. Halliwell B, GutteIidge JMC (1984) Oxygen toxidity, radicals, transition metal and disease. Biochem. j. 319:1-14
  12. Keinlinen M, Julkunen-Tiitto R (1996) Effect of sample preparation method on brich (Betula pendula Roth) leaf phenolics. J. Agri. Food Chem. 44:724-2727
  13. Kwok D, Shetty K (1997) Pseudomonas spp. -mediated regulation of total phenolic and rosmarinic acid levels in shoot-based clonal lines of thyme (Thymus Vulgaris L'). J. Food Biochem. 20:365-377 https://doi.org/10.1111/j.1745-4514.1996.tb00562.x
  14. Last RL, Conklin PL, K1iebenstein DJ (1997) Genetic approachs to studies of free radical detoxification in Arabidopsis. In Abstracts of Keystone Symposium in Metabolic Engineering in Transgenic Plants, Copper Mountain, Colorado, USA. p. 8
  15. Levone A, Tenhaken R, Dixon R, Lamb C (1994) $H_2O_2$ form the oxdative burst orchestrates the plant hypersensitive disease resistance response. Cell 79:583-593 https://doi.org/10.1016/0092-8674(94)90544-4
  16. Macheix JJ, FleuIiet A, Billot J (1990) Changes and metabolism of phenolics compounds in Fmits. In Fruit Phenolics, 1st ed.; Macheix JJ, Fleuriet A, Billot J, Eds; CRC Press: Boca Raton, FL, p. 149-221
  17. Melzoch K, Filip V, Buckiov D, Hanzlikov I, Smidrkal J (2000) Resveratrol occurrence in wine originating from Czech vineyard regions and effect on human health. Czech J Food Sci., 18(1):35-40.
  18. Peterson GL (1979) A simplification of the protein assay method of Lowry et al. which is more applicable. Analytical Biochemistry 83:346-356 https://doi.org/10.1016/0003-2697(77)90043-4
  19. Rein D, Paglieroni TG, Wun T, Pearson DA, Schmitz HH, Gosselin R, Keen CL (2000) Cocoa inhibits platelet activation and function. Am. J. Clin. Nutr. 72:30-35
  20. Rice-Evans CA, Miller NJ, Paganga G (1996) Structureantioxidant activity relationships of flavonoids and phenolic acids. Free Radical Biol. Med. 20:933-956 https://doi.org/10.1016/0891-5849(95)02227-9
  21. Stevenson PC, Anderson JC, Blaney WM, Simmonds MSJ (1993) by a novel caffeoylquinic acid from the wild groundnut, Arachis paraguariensis. J. Chem Ecol. 19:2917-2933 https://doi.org/10.1007/BF00980592
  22. Waterhouse AL, Walzem RL (1998) Nutrition of grape phenolics. In Flavonoids in Health and Disease; Rice-Evans CA, Packer L, Eds. Dekker: New York, USA. p. 359-385
  23. Wattenberg LW, Coccia]B, Lam LKT (1980) Inhibitory effects of phenolic-compounds on bezo(a)pyrene-induced neoplasia. Cancer Res. 40:2820-2823
  24. Woese K, Lange D, Boess C, Bogl KW (1997) A comparison of organically and conventionally grown foods-results of a review of the relevant literature. J. Sci. Food Agric. 74:281-293 https://doi.org/10.1002/(SICI)1097-0010(199707)74:3<281::AID-JSFA794>3.0.CO;2-Z
  25. Zhu Y, Coury LA, Long H, Duda CT, Kissinger CB, Kissinger PT(2000) Liquid chromatography with multichanel eleCtrochemical detection for the determination of resveratrol in wine, grape juice, and grape seed capsulen with automated solid phase extraction. J. Liq. Chromo Rel. Technol. 23(10):1555-1564 https://doi.org/10.1081/JLC-100100434