Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Production of Gomisin J from Cell Suspension Cultures of Schisandra chinensis Baillon

오미자의 현탁배양세포로부터 Gomisin J의 생산

  • 황성진 (동신대 식품생물공학과) ;
  • 표병식 (동신대 식품생물공학과) ;
  • 이학주 (국립산림과학원 화학미생물과) ;
  • 황백 (전남대 생물학과)
  • Published : 2004.12.31
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Abstract

Cell growth and gomisin J production by suspension cultures of Schisandra chinensis Baillon were investigated under various culture media, initial sucrose concentrations, shaking speeds, and inoculum sizes. Callus was induced from in vitro cultivated leaf segments on MS medium supplemented with $1\;mg/{\ell}$ NAA. The maximum dry cell weight of 2.23 g was obtained at inoculum size of 0.5 g fresh cell weight and in MB5 medium supplemented with $1\;mg/{\ell}$ NAA, 3% sucrose after 8 weeks. The production of gomisin J in suspension cell cultures was maximized in WPM medium containing 5% sucrose. The shaking speed for maintaining maximal cell dry weight was 100 rpm while the best shaking speed for gomisin J accumulation was 140 rpm.

Keywords

References

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