Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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In vitro Biological Activity Assay of Ethanol Extract of Radish

무 에탄올 추출물의 in vitro 생리활성 분석

  • Jung, Min-Suk (Department of Food and Biotechnology and Department of Innovative Industrial Technology, Hoseo University) ;
  • Lee, Gun-Soon (Department of Rural Living Science, Korea National Agricultural College) ;
  • Chae, Hee-Jeong (Department of Food and Biotechnology and Department of Innovative Industrial Technology, Hoseo University)
  • 정민숙 (호서대학교 벤처전문대학원 및 식품생물공학과) ;
  • 이건순 (한국농업전문대학 교양공통학과) ;
  • 채희정 (호서대학교 벤처전문대학원 및 식품생물공학과)
  • Published : 2004.03.31
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Abstract

In vitro biological activities of ethanol extract of radish including whitening, hangover removal, antimicrobial and antioxidant activities were analyzed. For whitening activity assay, tyrosinase inhibition rate was measured as $IC_{50}$ (50% inhibitory concentration). The $IC_{50}$ values of radish trunk and root extracts were estimated as 0,9 mg/ml and 2.1 mg/ml, respectively. Radish trunk extract showed 2.5-fold tyrosinase inhibition activity of radish root extract, however, there was no significant difference according to radish species. By alcohol dehydrogenase (ADH) activity assay as a hangover removal activity assay, radish trunk extract (2.5 mg/ml) and root extracts (8 mg/ml) showed ]50% activation of ADH. TBA values of radish trunk and root extracts (1% of each) were 43-61 % level of ${\alpha}-tochoperol$ (2.2%). From the analysis of in vitro biological activities of radish, it was suggested that radish could be used in functional food or cosmetics containing hangover removal, whitening and antioxidant activities.

무 에탄올 추출물의 미백기능, 숙취해소, 항균활성 및 항산화능을 분석하였다. Tyrosinase inhibition assay법을 이용하여 미백활법을 측정한 결과 무 줄기와 무 뿌리 추출물의 $IC_{50}$(50% inhibitory concentration)은 각각 0.9 mg/ml와 2.1 mg/ml를 나타냈다. 무뿌리 추출물보다 무줄기 추출물이 2.5배 정도 높은 tyrosinase의 저해율을 보였지만, 무의 품종별로는 별다른 활성 차이를 보이지 않았다. 숙취해소 활성의 분석법으로는 alcohol dehydrogenase activity assay방법을 이용하여 측정한 결과 ethanol을 분해시키는데 필요한 효소인 alcohol dehydrogenase를 150% 활성화시키는 무줄기와 무뿌리 추출물의 농도가 각각 2.5 mg/ml와 8 mg/ml로 나타났다. 항산화력의 직접적인 지표로 대표적으로 분석되는 항목인 TBARS법에 의한 TBA 값을 천연 항산화제와 비교한 결과, 무줄기 추출물과 무뿌리 추출물(1%)은 ${\alpha}-tochoperol$(2.2%)의 43-61% 수준의 항산화 활성을 나타냈다. 이와 같은 생리활성 분석을 통하여 무를 이용한 숙취해소 및 항암 기능식품 개발, 미백 기능 소재로의 개발 가능성을 확인할 수 있었다.

Keywords

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