Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Development of Biologically Active Compounds from Edible Plant Sources-VIII. - Isolation of Platelet Aggregation Inhibitory Compounds from the Arils of Euphoria longana L. -

식용 식물자원으로부터 활성물질의 탐색-VIII. - 용안육(Euphoria longana L.)으로부터 분리된 uridine의 혈소판 응집 저해 효과 -

  • Kim, Dong-Hyun (Graduate School of Biotechnology & Plant Metabolism Reserch Center, Kyung Hee University) ;
  • Song, Myoung-Chong (Graduate School of Biotechnology & Plant Metabolism Reserch Center, Kyung Hee University) ;
  • Choi, Jung-Min (Graduate School of East-West Medical Science, Kyung Hee University) ;
  • Kim, Sung-Hoon (Graduate School of East-West Medical Science, Kyung Hee University) ;
  • Kim, Dae-Keun (Department of Pharmacy, Woosuk University) ;
  • Chung, In-Sik (Graduate School of Biotechnology & Plant Metabolism Reserch Center, Kyung Hee University) ;
  • Park, Mi-Hyun (Erom Life Co. Ltd.) ;
  • Kwon, Byoung-Mog (Korea Research Institute of Bioscience and Biotechnology, KIST) ;
  • Baek, Nam-In (Graduate School of Biotechnology & Plant Metabolism Reserch Center, Kyung Hee University)
  • 김동현 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 송명종 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 최정민 (경희대학교 동서의학대학원) ;
  • 김성훈 (경희대학교 동서의학대학원) ;
  • 김대근 (우석대학교 약학대학) ;
  • 정인식 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 박미현 ((주)이롬라이프) ;
  • 권병목 (한국생명공학연구원) ;
  • 백남인 (경희대학교 생명공학원 및 식물대사연구센터)
  • Published : 2004.03.31
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Abstract

The arils of Euphoria longana L. was extracted with 80% aqueous MeOH, and the concentrated extract was partitioned with EtOAc, n-BuOH and $H_2O$. From the n-BuOH fraction, four compounds were isolated through the repeated silica gel, ODS and Sephadex LH-20 column chromatographies. From the result of physico-chemical data including NMR, MS and IR, the chemical structures of the compounds were determined as 1,1-dimethyl-2propenyl $1-O-{\beta}-D-glucopyranoside$, ethyl ,${\beta}-D-glucopyranoside$, 5-(hydroxymethyl)-2-furfur-aldehyde and uridine. Uridine exhibited inhibition effect of 79% on platelet aggregation at the concentration of$5\;{\mu}g/ml$.

용안육을 80% MeOH 용액으로 추출하고, 추출물을 EtOAc, n-BuOH 및 물로 분배, 추출하였다. 이 중 n-BuOH 분획을 silica gel, ODS 및 Sephadex LH-20 column chromatography로 정제하여 4종의 화합물을 분리하였다. 각 화합물의 화학구조는 NMR, MS및 IR 등의 스펙트럼 데이터를 해석하여, 1,1-dimethyl-2-propenyl $1-O-{\beta}-D-glucopyranoside$, ethyl ${\beta}-D-glucopyranoside$, 5-(hydroxymethyl)-2-furfuraldehyde 및 uridine으로 동정하였다. Uridine은 $5\;{\mu}g/ml$의 농도에서 collagen으로 유도한 혈소판 응집을 78% 저해하였다.

Keywords

References

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