Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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A Study on Hypoglycemic Effects of Crude Extracts of Schizandrae Fructus

오미자 추출물의 혈당 강하 효과에 관한 연구

  • Published : 2004.06.30
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Abstract

Hypoglycemic effect of Schizandrae Fructus (SF) extract containing in Okchun-san was determined on 3T3-L1 fibroblasts and adipocytes by investigating insulin-like activity, insulin sensitizing activity and ${\alpha}-glucoamylase$ suppressing activity. SF were extracted by using 70% ethanol followed by XAD-4 column chromatography with a mixture solvent of methanol and water, and the fractional extractions were utilized for assaying hypoglycemic effect. No inhibition of ${\alpha}-glucoamylase$ activity of SF was observed. Insulin-like activity 3T3-L1 adipocytes was not shown by SF. A significant insulin sensitizing activity of SF extractions was observed in 3T3-L1 adipocytes, giving SF extractions with 1 ng/ml insulin to reach glucose uptake level increased by 50 ng/ml of insulin alone. When cells were treated with SF (Fr. 4 or 5) plus 1 ng/ml insulin, glucose uptake was increased more than seven times as compared to 1 ng/ml of insulin alone, suggesting that SF extracts increased GLUT4 content by enhancing insulin signaling. These data suggest that SF extracts (especially Fr. 4 and 5) contains an effective insulin sensitizing compounds for hypoglycemic activity in 3T3-L1 adipocytes.

한의학에서 당뇨병 (소갈) 처방으로 사용되는 옥천산 처방의 성분 중의 하나인 오미자의 포도당 이용에 대한 효과를 조사하기 위해서 오미자를 70% 에탄올로 추출한 후 메탄올과 물을 섞은 용액으로 단계별로 XAD-4 column 으로 분획하였다. 오미자를 복용한 후 혈당을 낮추기 위해서는 인슐린처럼 작용하는 인슐린성 물질이거나, 인슐린 작용을 향상시키는 인슐린 민감성 물질로 작용하거나, 또는 ${\alpha}-glucoamylase$ 활성을 억제하여 탄수화물의 소화를 방해 작용이 있어야 하므로 본 연구에서는 섬유아세포와 지방세포 3T3-L1에서 각 분획층이 이러한 3가지 기전에 관여하는 지를 조사하였다. 오미자 분획물은 인슐린성 물질로 작용하거나 ${\alpha}-glucoamylase$의 활성을 저하시켜 탄수화물의 소화를 방해하지 않았다. 그러나 오미자 분획층은 인슐린의 작용을 향상시켜 포도당의 흡수를 증가시키는 효과가 매우 컸다. 특히 오미자 분획층 중 Fr. 4(메탄올 60%)와 Fr. 5(메탄올 80%)는 지방세포 3T3-L1에서 포도당의 흡수를 현저하게 증가시켜 인슐린을 50 ng/ml를 처리한 것보다도 효과적으로 포도당 흡수를 증가시켰다. Fr. 4와 Fr. 5에서 포도당 흡수가 증가한 것은 Fr. 4와 5가 인슐린 작용을 향상시켜 세포막에 GLUT4양을 증가시진 결과이었다. 결론적으로 오미자 중 특히 Fr. 4와 Fr. 5에 인슐린 민감성 제제가 함유되어 있을 것으로 추정된다.

Keywords

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