Content of Orotic Acid in Raw milk and Reduction of OA by Lactic Acid Bacteria during Fermentation

Orotic acid의 원유내 함량과 발효중 유산균에 의한 감소

  • Published : 2004.02.28

Abstract

Changes in erotic acid (OA) contents of raw milk and during cultivation and storage at refrigerated temperature were determined. OA contents of raw and 10% reconstituted milk held at $121^{\circ}C$ for up to 15 min decreased by 17.6 and 22.4%, and those fermented at 40 and $37^{\circ}C$ for 72 hr with Lactobacillus helveticus 166 and Lactobacillus casei 955 decreased 37.8-43.2 and 41.8-76%, whereas when fermented at $40^{\circ}C$ for 72 hr with Streptococcus thermophilus ST-37, did not decrease significantly compared with fermentation by other lactobacilli. OA content did not change during storage at refrigeration temperature. OA reduction by Lactobacillus sp. was dramatically increased at the initiation of stationary phase during fermentation. OA reduction varied among different lactobacilli. These results show decrease in cultivation time, rapid cooling of yogurt, and proper selection of Lactobacillus sp. prevent OA content reduction.

다양한 기능성 작용이 있는 물질로 알려진 OA에 대하여, 발효 과정에서 유산균에 의해 발생되는 손실을 줄이기 위한 방법을 찾고자 본 실험을 실시했다. 원유와 10% 환원유를 $121^{\circ}C$에서 15분간 열처리를 한 결과, OA의 감소율은 각자 17.6%, 22.4%, 정도로서 비교적 열에는 안정한 것으로 나타났다 발효 과정 중 유산균에 의한 OA의 변화를 측정한 결과 L. helveticus 166과 L. casei 955는 균수가 최고점에 도달하는 발효 24시간까지 OA의 감소는 미미하였으나 24시간 이후부터 72시간까지 각각 43.2%, 76%까지 OA가 급격히 감소하였고, S. thermophilus ST-37은 발효 72시간까지 OA의 감소가 미미하거나 오히려 생성하는 것으로 나타나 유산균의 종류에 따라 OA의 변화가 큰 것을 확인 할 수 있었다. 냉장 저장 중 OA함량은 유산균의 종류에 관계없이 변화가 없었다.

Keywords

References

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