Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Survey of Ochratoxin A in Cereal-based Korean Traditional Foods by HPLC

HPLC를 이용한 전통식품 중 오크라톡신 A 오염도 조사

  • Published : 2004.02.28
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Abstract

To determine rapid and reliable analytical method for ochratoxin A detection in cereal-based Korean traditional foods, ochratoxin A content was measured by high-performance liquid chromatography (HPLC) with immunoaffinity column clean-up. Recoveries of ochratoxin A in tested samples ranged from 68.4 to 85.3%. Occurrences of ochratoxin A were 15, 10, and 5% for Kochujang, Deonjang, and Kanjang, respectively. None was detected in Sunsik (mixed cereals). Average levels of ochratoxin A ranged from $0.5\;to\;1.3{\mu}g/kg$, lower than maximum residue level of $5-50{\mu}g/kg$ of ochratoxin A recorded in foreign food code.

국내 전통식품 중에 함유되어 있는 ochratoxin A를 검출할 수 있는 신속, 정확한 분석방법을 확립하고자 immunoaffinity column과 $C_{18}$ Sep-Pak cartridge를 비교 후 분리도와 회수율이 우수한 immunoaffinity column으로 전처리 방법을 사용하였으며 HPLC 검출한계는 $0.5{\mu}g/kg$로 나타났다. 오염도조사를 위하여 시판되는 장류(고추장, 된장, 간장 각 20건) 60건을 수거하여 분석한 결과 고추장 3건, 된장 2건, 간장 1건에서 $0.5-1.3{\mu}g/kg$ 수준으로 검출되었으며 수거된 선식 40건에서는 ochratoxin A가 검출한계이하 수준이었다. 이는 일부 외국의 규격치인 $5-50{\mu}g/kg$와 비교해 볼 때 낮은 수준이었다.

Keywords

References

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