Screening of the Antioxidant Activity of Some Medicinal Plants

항산화활성을 나타내는 약용식물 소재 탐색

  • Published : 2004.04.30

Abstract

Antioxidant activities of water extracts of 20 medicinal plants (1 mg/mL) on peroxidation of linolic acid were evaluated by thiocyanate method, among which 11 showed strong antioxidant activity (> 70%). Higher hydroxy radical scavenging activity (> 60%) were shown in Corner officinalis, Acanthopanax sessiliflorus, and Epimedium koreanum than the other plants. Epimedium koreanum than the other plants extract showed highest superoxide radical scavenging activity (42%). Total polyphenol contents ranged from $2.6\;(Polygonatum\;odoratum){\sim}81.2(Epimedium\;koreanum)\;mg/g$. Direct correlation between the antioxidant activity and polyphenol content (r=0.8) was established through simple regression analysis. $IC_{50}$ for selected four plant extracts, showing highest polyphenol contents and antioxidant activities, were significantly higher than positive control. Total antioxidant activity of vitamin c was significantly lower than those of Acanthopanax sessiliflorus, Epimedium koreanum, and Erythrina variegata. Superoxide radical scavenging activity of Acanthopanax sessiliflorus was similar to BHA. Results suggest water extracts of some medicinal plants could be potential candidates for natural antioxidants.

20종 약용식물 물 추출물의 항산화활성 및 이의 폴리페놀화합물과 플라보노이드 함량을 측정 비교하였다. Linoleic acid emulsion을 이용한 total antioxidant 활성에서는 1 mg/mL 농도에서 음양곽, 오미자, 파고지, 당귀, 해동피, 토사자, 감초, 갈근, 오가피, 속단 및 산수유가 대조구에 비해 70%이상의 항산화활성을 나타내었다. 수소이온 라디칼 소거능은 오가피, 음양곽 및 산수유가 60%이상의 활성을 나타내었으며 황기는 활성이 나타나지 않았고 경포부자, 인삼, 삼칠근 및 황정은 5%이하의 매우 낮은 활성을 나타내었다. Superoxide 이온 라디칼 소거능은 수소이온 소거능 보다 활성이 낮아 1 mg/mL 농도에서는 거의 모든 시료에서 활성이 관찰되지 않았으며 5mg/mL 농도에서 음양곽이 약 42%로 가장 높았고 해동피, 오가피, 파고지, 토사자 및 속단이 20%이상의 비교적 높은 활성을 나타내었다. 총 폴리페놀은 옥죽이 약 2.6 mg/g으로 가장 작고 음양곽이 약 81.2 mg/g으로 가장 많았으며, 플라보노이드 함량은 황정이 약 0.4 mg/g으로 가장 적고, 감초가 약 55.4 mg/g으로 가장 많았다 실험에 사용한 식물 대부분에서 폴리페놀의 함량이 플라보노이드보다 많았으며, 시료 추출물의 항산화활성과 폴리페놀 함량을 비교한 결과 각 활성에 대하여 2-3종의 시료를 제외한 나머지에서 모두 폴리페놀 함량과 활성간에 양의 상관관계를 나타내어($r{\fallingdotseq}0.8$) 식물 중에 존재하는 폴리페놀화합물들이 그들이 나타내는 항산화활성과 밀접한 관련이 있음을 확인할 수 있었다. 측정한 세 가지 활성 모두에서 환성이 높고 폴리페놀화합물의 양도 많은 산수유, 오가피, 음양곽 및 해동피의 각 활성에 대한 $IC_{50}$을 측정한 결과 오가피가 세 가지 활성 모두에서 가장 낮은 값을 나타내어 활성이 가장 높았으며 음양곽 또한 superoxide 라디칼 소거능을 제외하고는 오가피와 거의 유사한 활성을 나타내었다. Positive control로 사용한 비타민 C, E, BHA 및 BHT의 경우 BHA의 superoxide 라디칼 소거능을 제외한 나머지는 모두 실험에 사용한 식물들에 비하여 $IC_{50}$ 이유의적으로 낮았다 그러나 total antioxidant 활성에서 오가피, 음양곽, 해동피는 비타민 C보다 유의적으로 높았고 산수유는 유사하였으며, superoxide 라디칼 소거능도 오가피가 BHA에 준하는 활성을 나타내어 이들 식물이 천연 항산화제로 사용이 가능할 것으로 기대되었다.

Keywords

References

  1. Ames BN, Shigenaga MK, Hagen TM. Oxidants, antioxidants, and the degenerative diseases of aging. Proc. Natl. Acad. Sci. USA 90: 7915-7922 (1993) https://doi.org/10.1073/pnas.90.17.7915
  2. Dean RT, Gieseg, Davies MJ. Reactive species and their accumulation on radical damaged proteins. Trends Biochem. Sci. 18: 437-441 (1993) https://doi.org/10.1016/0968-0004(93)90145-D
  3. Branen AL. Toxicology and biochemistry of butylated hydroxyanisole and butylated hydroxytoluene. J. Am. Oil. Chem. Soc. 52: 59-63 (1975) https://doi.org/10.1007/BF02901825
  4. Farag RS, Badei AZMA, Hewedi FM, El-Baroty GSA. Antioxidant activity of some spice essential oils on linoleic acid oxidation in aqueous media. J. Am. Oil. Chem. Soc. 66: 792-799 (1989) https://doi.org/10.1007/BF02653670
  5. Farag RS, Badei AZMA, El-Baroty GSA. Influence of thyme and clove essential oils on cottonseed oil oxidation. J. Am. Oil. Chem. Soc. 66: 800-804 (1989) https://doi.org/10.1007/BF02653671
  6. Yu JH, Cho CM, Oh DH, Pyun YR. Antioxidant properties of red-pepper peel extracts on margarine. Korean J. Appl. Microbiol. Bioeng. 9: 21-27 (1981)
  7. Maeng YS, Park HK. Antioxidant activity of ethanol extract from Dodok (Codonopsis lanceolata). Korean J. Food Sci. Technol. 23: 311-316 (1991)
  8. Park JH, Kang KC, Baek SB, Lee YH, Rhee KS. Separation of antioxidant compounds from edible marine algae. Korean J. Food Sci. Technol. 23: 256-261 (1991)
  9. Cheigh HS, Lee JS, Moon GS, Park KY. Antioxidative characteristics of fermented soybean sauce on the oxidation of fatty acid mixture. Korean J. Food Sci. Technol. 22: 332-336 (1990)
  10. Moon GS, Ryu BM, Lee MJ. Components and antioxidative activities of Buchu (Chinese chives) harvested at different times. Korean J. Food Sci. Technol. 35: 493-498 (2003)
  11. Lee JS, Lee SW. The studies of composition of fatty acids and antioxidnat activities in parts of Omija (Schizandra chinensis Baillon). Korean J. Diet. Cult. 6: 147-153 (1991)
  12. Hong JS, Choi JS, Jung KT, Joo IO. The antioxidative, antimicrobial and nitrite scavenging effects of Schizandra chinensis RUPRECHT (Omija) seed. Korean J. Food Sci. Technol. 32: 928-935 (2000)
  13. Kim DG, Son DH, Choi UK, Cho YS, Kim SM. The antioxidant ability and nitrite scavenging ability of Poria cocos. J. Korean Soc. Food Sci. Nutr. 31: 1097-1101 (2002) https://doi.org/10.3746/jkfn.2002.31.6.1097
  14. Oh MJ, Son HY, Kang JC, Lee KS. Antioxidative effect of Pueraria root extract on edible oils and fats. J. Korean Soc. Food Nutr. 19: 448-456 (1990)
  15. Choi J, Shin DH, Chang YS, Shin JI. Screening of natural antioxidant from plant and their antioxidative effect. Korean J. Food Sci. Technol. 24: 142-148 (1992)
  16. Kim SY, Kim JH, Ki SK, Oh MJ, Jung MY. Antioxidant activities of selected oriental herb extracts. J. Am. Oil. Chem. Soc. 71: 633-640 (1994) https://doi.org/10.1007/BF02540592
  17. Kannel WB, Hjortlane MC, Mcnamara PM, Gordon T. Menopause and risk of cardiovascular disease. The framingham study. Ann. Int. Med. 85: 447-452 (1976) https://doi.org/10.7326/0003-4819-85-4-447
  18. Grodstein F, Stampfer MJ, Manson JE, Colditz GA, Willett WC, Rosner B. Postmenopausal estrogen and progestin use and the risk of cardiovascular disease. N. Engl. J. Med. 335: 453-461 (1996) https://doi.org/10.1056/NEJM199608153350701
  19. Sack MN, Rader DJ, O'Cannon RO. Estrogen and inhibition of oxidation of low-density lipoproteins in postmenopausal women. Lancet 343: 269-270 (1994) https://doi.org/10.1016/S0140-6736(94)91117-7
  20. Mitsuda H, Yasumoto K, Iwami K. Antioxidative action of indole compounds during the autoxidation of linoleic acid. Eiyo to Shokuryo 19: 210-214 (1966) https://doi.org/10.4327/jsnfs1949.19.210
  21. Brand-Williams W, Cuvelier ME, Barnes G. Use of a free radical method to evaluate antioxidant activity. Lebensm. Wiss. Technol. 28: 25-30 (1985)
  22. Crapo JD, Mccord JM, Fridovich I. Preparation and assay of superoxide dismutases. Methods Enzymol. 53: 382-393 (1978) https://doi.org/10.1016/S0076-6879(78)53044-9
  23. Nakabayashi T. Studies on tannin of fruits and vegetables. Nippon Shokuhin Gakkaishi 15: 73-76 (1968) https://doi.org/10.3136/nskkk1962.15.73
  24. NFRI. Manuals of Quality Characteristic Analysis for Food Quality Evaluation (2). National Food Research Institute, Skuba, Japan. p. 61 (1990)
  25. SAS Institute, Inc. SAS User's Guide. Statistical Analysis System Institute, Cary, NC, USA (1992)
  26. Sato M, Ramarathnam N, Suzuki Y, Ohkubo T, Takeuchi M, Ochi H. Varietal differences in the phenolic content and superoxide radical scavenging potential of wines from different sources. J. Agric. Food Chem. 44; 37-41 (1996) https://doi.org/10.1021/jf950190a
  27. Bors W, Saran, M. Radical scavenging by flavonoid antioxidants. Free Rad. Res. Comm. 2: 289-294 (1987) https://doi.org/10.3109/10715768709065294
  28. Fitzpatrick DF, Hirschfield SL, Coffey RG. Endothelium-dependent vasorelaxing activity of wine and other grape products. Am. J. Physiol. 265: H774-H778 (1993)
  29. Hussain SR, Cillard J, Cillard P. Hydroxyl radical scavenging activity of flavonoids. Phytochemistry 26: 2489-2491 (1987) https://doi.org/10.1016/S0031-9422(00)83860-1
  30. Afanaslev IB, Dorozhko AI, Bordskii AV. Chelating and free radical scavenging mechanisms of inhibitory action of rutin and quercetin in lipid peroxidation. Biochem. Pharmacol. 38: 1763-1769 (1989) https://doi.org/10.1016/0006-2952(89)90410-3
  31. Torel J, Cillard J, Cillard P. Antioxidant activity of flavonoids and reactivity with peroxy radicals. Phytochemistry 25: 383-385 (1986) https://doi.org/10.1016/S0031-9422(00)85485-0