Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
권호 표지

Optimization for the Production of Mono- and Di-acylglycerols from Corn Oil by Enzymic Glycerolysis Using Response Surface Methodology

반응표면분석에 의한 옥수수유 유래 monoacylglycerol과 diacylglycerol 합성 조건의 최적화

  • Park, Rae-Kyun (Department of Food Science and Technology, Chungnam National University) ;
  • Choi, Sang-Won (Department of Food Science and Nutrition, Catholic University of Daegu) ;
  • Lee, Ki-Teak (Department of Food Science and Technology, Chungnam National University)
  • 박래균 (충남대학교 식품공학과) ;
  • 최상원 (대구가톨릭대학교 식품영양학과) ;
  • 이기택 (충남대학교 식품공학과)
  • Published : 2004.10.31
Download PDF
⟨ Previous Next ⟩

Abstract

Response surface methodology was used to optimize production conditions of monoacylglycerol (MAG) and diacylglycerols (DAG) from corn oil by enzymic glycerolysis. Contents of $1,3-DAG\;(Y_1),\;1,2-DAG\;(Y_2),\;total\;DAG\;(Y_3),\;MAG\;(Y_4)$, and total $DAG+MAG\;(Y_5)$ were obtained. Conditions were optimized using central composite design with incubation temperature $(35-75^{\circ}C,\;X_1)$, incubation time (1-11 hr, $X_2$), and amount of hexane added (0-2 mL, $X_3$) as three variables. Content of 1,3-DAG was maximized by 20.43 area% at incubation temperature of $44.92^{\circ}C$, incubation time of 10.24 hr, and hexane content of 1.16 mL, whereas that of 1,2-DAG (26.78 area%) was maximized at $56.32^{\circ}C$, 6.95 hr, and 1.04 mL, respectively. Predicted maximum total DAG content was 45.09 area% at $53.82^{\circ}C$, 8.03 hr, and 1.08 mL, while production conditions of MAG (9.57 arae%) were $64.14^{\circ}C$, 7.00 hr, and 0.13 mL. At variables of $54.07^{\circ}C$, 7.98 hr, and 1.02 mL, maximum content of total DAG+MAG predicted by RSM was 53.54 area%.

Lipozyme RM IM 효소적 촉매를 사용한 glycerolysis에 의한 옥수수유로부터 $1,3-DAG(Y_1),\;1,2-DAG(Y_2)$, 총 $DAG(Y_3),\;MAG (Y_4)$, 및 총 DAG + MAG 의 합성 양$(Y_5)$의 생성 최적화를 반응 표면분석을 이용하여 모니터링 하였으며 반응온도$(35-75^{\circ}C,\;X_1)$, 반응시간$(1-11시간,\;X_2)$ 및 hexane 의 혼합 양$(0-2mL,\;X_3)$을 변수로 한 중심합성계획으로 최적화 실험하였다. 1,3-DAG는 반응온도$(44.92^{\circ}C)$, 반응시간(10.24시간) 및 hexane 첨가량(1.16mL) 조건에서 20.43 area%로 최대값을 나타내었으며 1,2-DAG는 $56.32^{\circ}C$, 6.95시간 및 1.04mL 조건에서 26.78 area% 로 최대값을 예측할 수 있었다. 총 DAC는 $53.82^{\circ}C$, 8.03시간 및 1.08mL 실험 조건에서 45.09 area% 최대값을 보였다. 또한 MAG 생산 최적조건은 $64.14^{\circ}C$, 7.00시간 및 0.13mL에서 최대값 9.57 area%로 예측되었으며 총 DAG+MAG 생산 최적조건은 $54.07^{\circ}C$, 7.98시간, 1.02mL이었으며 이때 예측된 값은 53.54 area%이었다.

Keywords

References

  1. Kang ST, Yamane T. Effect of temperature on diacylglycerol production by enzymatic solid-phase glycerolysis of hydrogenated beef tallow. Korean J. Food Sci. Technol. 26: 567-572 (1994)
  2. Maki KC, Davidson MH, Tsushima R, Matsuo N, Tokimitsu I, Umporowicz DM, Dicklin M R, Foster GS, Ingram KA, Anderson BD, Forst SD, Bell M. Consumption of diacylglycerol oil as part of a reduced-energy diet enhances loss of body weight and fat in comparison with consumption of triacylglycerol control oil. Am. J. Clin. Nutr. 76: 1230-1236 (2002) https://doi.org/10.1093/ajcn/76.6.1230
  3. AOCS. DAG and Food Formulation. p. 550. American Oil Chemical Society, Champaign, IL, USA (2002)
  4. Lee JS, Jang Y, Yang TH. Low-calorie Structured Lipids Synthesis by Enzymatic Transesterification. Korea Ministry of Agriculture and Forestry, Kyonggi, Korea. pp. 9-17 (1999)
  5. Kwon SJ, Han JJ, Rhee JS. Production and in situ separation of mono- or diacylglycerol catalyzed by lipase in n-hexane. Enzyme Microb. Technol. 17: 700-704 (1995) https://doi.org/10.1016/0141-0229(94)00117-A
  6. Yang B, Harper WJ, Parkin KL, Chen J. Screening of commer-cial lipases for production of mono- and diacylglycerols from butter oil by enzymic glycerolysis. Int. Dairy J. 4: 1-13 (1994) https://doi.org/10.1016/0958-6946(94)90045-0
  7. Rosu R, Uozaki Y, Iwasaki Y, Yamane T. Repeated use of immo-bilized lipase for monoacylglycerol production by solid-phase glycerolysis of olive oil. J. Am. Oil Chem. Soc. 74: 445-450 (1997) https://doi.org/10.1007/s11746-997-0104-2
  8. Boruscheuer UT. Lipase-catalyzed syntheses of monoacylglycerols. Enzyme Microb. Technol. 17: 578-586 (1995) https://doi.org/10.1016/0141-0229(94)00096-A
  9. Park KJ, Ahn EY, Kwon GS, Kim KS, Kang ST. Diacylglycerol production by enzymatic glycerolysis of soybean oil. Korean J. Microbiol. Biotechnol. 32: 84-90 (2004)
  10. Lee GD, Lee JE, Kwon JH. Application of response surface methodology in food industry. Food Sci. Ind. 33: 33-45 (2000)
  11. Sung NK. SAS/STAT Regression Analysis. Freedom Academy, Seoul, Korea. pp. 237-286 (2000)
  12. Jeong YJ, Lee GD, Kim KS. Optimization for the fermentation condition of persimmon vinegar using response surface methodology. Korean J. Food Sci. Technol. 30: 1203-1208 (1998)
  13. Bae DK, Choi HJ, Son JH, Park MH, Bae JH, An BJ, Bae MJ, Choi C. Optimization for the process of ethanol extracts of persimmon leaf using response surface methodology. J. Korean Soc. Agric. Chem. Biotechnol. 43:218-224 (2000)
  14. Lee DS, Noh BS, Bae SY, Kim K. Characterization of fatty acids composition in vegetable oils by gas chromatography and chemometrics. Anal. Chim. Acta. 358: 163-175 (1998) https://doi.org/10.1016/S0003-2670(97)00574-6
  15. Naz S, Sheikh H, Siddiqi R, Sayeed SA. Oxidative stability of olive, corn and soybean oil under different conditions. Food Chem. 88: 253-259 (2004) https://doi.org/10.1016/j.foodchem.2004.01.042
  16. A Food Distribution Yearbook. Food Journal, Seoul, Korea. pp. 292-299 (2002)
  17. Lee GD, Kim JS, Kwon JH. Monitoring of dynamic changes in maillard reaction substrates by response surface methodology. Korean J. Food Sci. Technol. 28: 212-219 (1996)
  18. SAS Institute, Inc. SAS User's Guide. 4th ed., Vol. 2. Statistical Analysis System Institute, Cary, NC, USA (1998)
  19. Foglia TA, Jones KC. Quantitation of neutral lipid mixtures using HPLC with light scattering detection. J. Liq. Chromatogr. Rel. Technol. 20: 1829-1838 (1997) https://doi.org/10.1080/10826079708005545
  20. Bae DK, Choi HJ, Son JH, Park MH, Bae JH, An BJ, Bae MJ, Choi C. Optimization for the process of extracts of persimmon leaf using response surface methodology. J. Korean Soc. Agric. Chem. Biotechnol. 43: 218-224 (2000)
  21. Ferreira-Dias S, Correia AC, Baptista FO, Fonseca MMR. Contribution of response surface design to the development of glycerolysis systems catalyzed by commercial immobilized lipases. J. Mol. Catal. Enz. 11: 699-711 (2001) https://doi.org/10.1016/S1381-1177(00)00079-5
  22. Park RK, Lee KT. Synthesis and characterization of mono- and diacylglycerol enriched functional oil by enzymatic glycerolysis of corn oil. Korean J. Food Sci. Technol. 36: 211-216 (2004)