Journal of Korean Medical Science

Korean Acad Medical Sciences (대한의학회)
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Effects of pH on Vascular Tone in Rabbit Basilar Arteries

Kim, Young-Chul;Lee, Sang-Jin;Kim, Ki-Whan

  • Published : 20040200

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Abstract

Effects of pH on vascular tone and L-type $Ca^{2+}$ channels were investigated using Mulvany myograph and voltage-clamp technique in rabbit basilar arteries. In rabbit basilar arteries, high $K^+$ produced tonic contractions by $11{\pm}0.6$ mN ($mean{\pm}S.E$., n=19). When extracellular pH ($pH_o$ ) was changed from control 7.4 to 7.9 ($[alkalosis]_o$), $K^+$-induced contraction was increased to $128{\pm}2.1$% of the control (n=13). However, $K^+$-induced contraction was decreased to $73{\pm}1.3%$ of the control at $pH_o$ 6.8 ($[acidosis]_o$, n=4). Histamine ($10\;{\mu}M$) also produced tonic contraction by $11{\pm}0.6\;mN$ (n=17), which was blocked by post-application of nicardipine ($1\;{\mu}M$). $[alkalosis]_o\;and\;[acidosis]_o$ increased or decreased histamine-induced contraction to $134{\pm}5.7%\;and\;27{\pm}7.6%$ of the control (n=4, 6). Since high $K^+$- and histamine-induced tonic contractions were affected by nicardipine and $pH_o$, the effect of $pH_o$ on voltage-dependent L-type $Ca^{2+}$ channel ($VDCC_L$) was studied. $VDCC_L$ was modulated by $pH_o$: the peak value of $Ca^{2+}$ channel current ($/_{Ba}$) at a holding of 0 mV decreased in $[acidosis]_o\;by\;41{\pm}8.8%$, whereas that increased in $[alkalosis]_o\;by\;35{\pm}2.1%$ (n=3). These results suggested that the external pH regulates vascular tone partly via the modulation of VDCC in rabbit basilar arteries.

Keywords

References

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