Journal of Korean Medical Science

Korean Acad Medical Sciences (대한의학회)
권호 표지

Development of a Rat Model of Graded Contusive Spinal Cord Injury Using a Pneumatic Impact Device

Yeo, Sang-Jun;Hwang, Sung-Nam;Park, Seung-Won;Kim, Young-Baeg;Min, Byung-Kook;Kwon, Jeong-Taik;Suk, Jong-Sik

  • Published : 20040800

⟨ Previous Next ⟩

Abstract

An animal model of spinal cord trauma is essential for understanding the injury mechanisms, cord regeneration, and to aid the development of new therapeutic modalities. This study focused on the development of a graded experimental contusion model for spinal cord injury (SCI) using a pneumatic impact device made in Korea. A contusive injury was made to the dorsal aspect of the cord. Three trauma groups were defined according to the impact velocity (IV). A control group (n=6), received laminectomy only. Group 1 (n=10), 2 (n=10), and 3 (n=10) had IVs of 1.5 m/sec, 2.0 m/sec, and 3.5 m/sec respectively. Functional assessments were made up to the 14th day after injury. The cord was removed at the 14th postinjury day and prepared for histopathologic examination. Significant behavioral and histopathological abnormalities were found in control and each trauma group. All trauma groups showed severe functional impairment immediately after injury but following different rates of functional recovery (Fig. 5). As the impact velocity and impulse increased, the depth of contusive lesion revealed to be profound the results show that the rat model reproduces spinal cord lesions consistently, has a distinctive value in assessing the effects of impact energy.

Keywords

References

  1. An overview of spinal cord injury models;Neurotrauma Andrew, R.B.;Povlishock, J.T.(ed.)
  2. Neurosurgery v.50 Graded contusion model of the mouse spinal cord using a pneumatic impact device Seki, T.;Hida, K.;Tada, M.;Koyanagi, I.;Iwasaki, Y. https://doi.org/10.1097/00006123-200205000-00024
  3. J Neurosci Methods v.39 A controlled cortical impact model of traumatic brain injury in the rat Dixon, C.E.;Clifton, G.L.;Lighthall, J.W.;Yaghmai, A.A.;Hayes, R.L. https://doi.org/10.1016/0165-0270(91)90104-8
  4. J Neurotrauma v.5 Controlled cortical impact: A new experimental brain injury model Lighthall, J.W.
  5. J Neurotrauma v.6 Experimental models of brain injury Lighthall, J.W.;Dixon, C.E.;Anderson, T.E.
  6. J Neurosurg v.80 A new model of diffuse brain injury in rats. Part I: Pathophysiology and biomechanics Marmarou, A.;Foda, M.A.;van den Brink, W.;Campbell, J.;Kita, H.;Demetriadou, K.
  7. J Neurosurg v.67 A fluid percussion model of experimental brain injury in the rat Dixon, C.E.;Lyeth, B.G.;Povlishock, J.T.;Findling, R.L.;Hamm, R.J.;Marmarou, A.;Young, H.F.;Hayes, R.L.
  8. J Korean Neurosurg Soc v.32 Development of upgraded cortical impact model (Part I: Mechanics) Choi, S.M.;Suk, J.S.;Kwon, J.T.;Min, B.K.;Kim, Y.B.;Hwang, S.N.;Choi, D.Y.;Kim, J.H.;Lee, S.M.;Earmme, Y.Y.
  9. J Korean Neurosurg Soc v.32 Development of upgraded cortical impact model (Part II: Functional outcome) Choi, S.M.;Suk, J.S.;Min, B.K.;Hwang, S.N.;Kim, Y.B.;Kim, J.H.
  10. Exp Neurol v.139 Graded histological and locomotor outocmes after spinal cord contusion using the NYU weightdrop device versus transection Basso, D.M.;Beattie, M.S.;Bresnahan, J.C. https://doi.org/10.1006/exnr.1996.0098
  11. J Neurotrauma v.12 A sensitive and reliable locomotor rating scale for open field testing in rats Basso, D.M.;Beattie, M.S.;Bresnahan, J.C.
  12. J Neurotrauma v.15 A mouse model of graded contusive spinal cord injury Kuhn, P.L.;Wrathall, J.R.
  13. J Neurol Sci v.37 An electron-microscopic analysis of axonal alterations following blunt contusion of the spinal cord of the rhesus monkey (Macaca mulatta) Bresnahan, J.C. https://doi.org/10.1016/0022-510X(78)90228-9
  14. J Neurol Sci v.28 A neuroanatomical analysis of spinal cord injury in the rhesus monkey (Macaca mulatta) Bresnahan, J.C.;King, J.S.;Martin, G.F.;Yashon, D. https://doi.org/10.1016/0022-510X(76)90122-2
  15. J Neurotrauma v.17 Traumatic spinal cord injury produced by controlled contusion in mouse Jakeman, L.B.;Guan, Z.;Wei, P.;Ponnappan, R.;Dzwonczyk, R.;Popovich, P.G.;Stokes, B.T.
  16. Exp Neurol v.169 Behavioral and histological outcomes following graded spinal cord contusion injury in the C57B1/6 mouse Ma, M.;Basso, D.M.;Walters, P.;Strokes, B.T.;Jakeman, L.B. https://doi.org/10.1006/exnr.2001.7679
  17. Exp Neurol v.88 Spinal cord contusion in the rat: Morphometric analyses of alteration in the spinal cord Noble, L.J.;Wrathall, J.R. https://doi.org/10.1016/0014-4886(85)90119-0