Journal of Korean Medicine Rehabilitation (한방재활의학과학회지)

The society of Korean Medicine Rehabilitation (한방재활의학과학회)
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Effects of Cheongyeolsaseup-tang(Qingrexiesh-tang) in Experimental Medel of Rheumatoid Arthritis

생체외 류마토이드 관절염 실험 모델에서 淸熱瀉濕湯의 효과

Park, Jin-Sang;Han, Myeong-Geum;Im, Yang-Ui;Lee, Su-Gyeong;Sin, Byeong-Cheol;Gwon, Yeong-Dal;Song, Yong-Seon
박진상;한명금;임양의;이수경;신병철;권영달;송용선

  • Published : 20040000

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Abstract

Objective : This study was designed to understand the mechanism of action of Cheongyeolsaseup-tang(Qingrexieshi-tang) (CYSST) leading to control of mast cells on inflamatory condition like RA. Methods : Viability of cells was evaluated by MTT colorimetric assay for 8 h incubation after stimulation of PMA plus A23187. HMC-1 cells (3$\times$105 cells/$m\ell$) were stimulated for 8 hour incubation with PMA plus A23187, we invesitigated the effect of CYSST on the production of cytokines by activsted human mast cell line HMC-1. Results : When CYSST (1 mg/ml) was added, the production of tumor necrosis factor(TNF)-$\alpha$, interleukin (IL)-6 was significantly inhibited by 37% and 33.6%, respectivity in phorbol 12-myristate 12-acetate plus calcium ionophore A23187-stimulated HMC-1 cells. RA is an aggressive inflammatory disease in which chemokines are thought to recruit leukocytes and induce angiogenesis. CYSST also inhibited by 48% the production of IL-8 in HMC-1 cells. In addition, CYSST inhibited the expression of Nuclear factor-$\textsc{k}$B(NF-$\textsc{k}$B) in HMC-1 cells. Conclusions : When CYSST was added, the production of TNF-$\alpha$, IL-6, IL-8 was significantly inhibited. These results imply that CYSST has a critical role on the treatment of the bone damage associated with rheumatoid arthritis.

Keywords

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