Journal of Korean Medicine Rehabilitation (한방재활의학과학회지)

The society of Korean Medicine Rehabilitation (한방재활의학과학회)
권호 표지

Anti-pathogenetic and Curative Effects of Taeganghwal-tang(Daqianghuo-tang) on the Collagen-Induced Arthritis in Wistar Rats

大羌活湯이 Collagen으로 유발된 관절염의 발생 및 치료에 미치는 영향

Jeong, Su-Hyeon;Kim, Sung-Jung;Seo, Il-Bok
정수현;김순중;서일복

  • Published : 20040000

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Abstract

Objectives : This study was carred out to investigate the anti-pathogenetic and curative effects of Taeganghwal-tang(Daqianhuo-tang) on collagen-induced arthritis on Wistar rats. Methods : Experiment I :Thirty male rats were divided into non-treated normal group, porcine type II collagen immunized control group, and collagen immunized and treated group medicated with extract of Taeganghwal-tang(Daqianghuo-tang) for 15days after collagen immunization. Body weight. paw edema volumem and thickness of ankle joint were measured at 0, 10, 15days after immunization. Arthritic score was evaluated at 11, 12, 13, 14, 15days after immunization. At 15days after immunization, the incidence of arthritis was evaformed on the ankle joint. Experiment II ; Thirteen rats, that were shown the gross lesions of collagen-induced arthritis, were divided into control group and treated group medicated with extract of Taeganghwal-tang(Daqianghuo-tang) for 15 days after onset of arthritis. Non immunized seven rats were served as normal group. Body weight, paw edema volume, and thickness of ankle joint were measured at 0, 5, 10, days after treatment, serum TNF-${\alpha}$, IL-1${\beta}$ and anti-collagen lgG level were analysed, and histopathological examination was performed on the ankle joint.Results : Exprtiment I ; -. Incident of arthritis was 60% in control group and 10% oin treated group. -. Arthritic score of treated group(0.3${\pm}$0.9 at 14days, 0.3${\pm}$0.9 at 15days) was significantly decreased(P<0.01) compared with control group(3.0${\pm}$ 2.7at 3.61${\pm}$3.1 at 14days, 3.9${\pm}$3.4 at 15days)respectively. -. Paw edema volume($m{\ell}$ of treated group(1.90${\pm}$0.11) was significantly decreased(P<0.01) compared with control group(2.55${\pm}$0.53) at 15days after immunization. -. Thickness of ankle joint(mm) of treted group(6.64${\pm}$0.15) was significantly decreased(P<0.01) compared was control group(8.35${\pm}$1.75) at 15 days after immunization. -. Serum TNF-${\alpha}$ level(pg/ml) of reated group(46.00${\pm}$25.90) was significantly decreased(P<0.05) compared with control group(97.52${\pm}$49.97) at 15 days after immunization. -.Serum IL-1${\alpha}$ level(pg/ml) of reated group(39.40${\pm}$10.42) was significantly decreased(P<0.05) compared with control group(52.22${\pm}$15.32) at 15 days after immunization. -Serum anti-collagen lgG level(mg/ml) of reated group(0.27${\pm}$0.16) was significantly decreased(P<0.05) compared with control group(0.47${\pm}$0.20) at 15 days after immunization. Experiment II ; -. Paw edema volume(ml) of treated group(3.12${\pm}$0.28 at 10days, 3.12${\pm}$0.27) at 15days) was significnatly decreased(P<0.05) compared with control group(3.72${\pm}$0.49 at 10days, 3.62${\pm}$0.48 at 15days) respectively. -. Serum TNF-${\alpha}$ level(pg/ml) of treated group(84.64${\pm}$22.04) was significantly decreased(P<0.05) compared with control group(135.31${\pm}$55.56) at 15days after treatment. -. Serum IL-${\beta}$ level(pg/ml) of treated group(58.16${\pm}$7.28) was not significantly different compared with control group(66.75${\pm}$10.68) at 15days after treatment. -. Serum anti-collagen lgG level(mg/ml) of treated group(0.78${\pm}$0.09) was not significantly different compared with control group(0.87${\pm}$0.08) at 15days after treatment. -. Serum anti-collagen lgG level(mg/ml) of treated group(0.78${\pm}$0.09) was not significantly different compared with control group(66.75${\pm}$10.68) at after treatment. -. Serum anti-collagen lgG level(mg/ml) of treated group(0.78${\pm}$0.09) was not significantly different compared with control group(0.87${\pm}$0.08) at 15 days after treatment. -. Histopathologically, destructive lesions of articular cartilage and subchondral bony tissue, and degree of fibrous ankylosis in treated group were alleviated compared with those of control group at 15 days after treatment. Conclusions : These results inducated that taeganghwal-tang(Daqianghuo-tang) has the anti-pathogenetic effects on the onset of collagen-inducde arthritis in rets, by it's inhibitory effects on the production of anti-collagen lgG. And also, Taeganghwal-tang(Daqianghuo-tang) has inhibitory effects on the progression of collagen-induced arthritis in rets, by i'ts lowering effects on the secretion of TNF-${\alpha}$.

Keywords

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